Changes of GABAA receptor activation kinetics in hippocampal neurons cultured for different periods of time

• Autorzy: Mozrzymas J W , Barberis A.
• Języki publikacji: EN

Abstrakty

EN

Cell culture is a convenient model for pharmacokinetic studies, but during the culture period, GABAa receptors are likely to undergo different modulatory processes. In this study, the current responses to ultrafast GABA applications were recorded from patches excised from neurons cultured for either up to two days (short-term culture) or for more than two weeks (long-term culture). The dose-dependencies of the currentrising phases revealed significant differences between the two groups. In the short-term cultures, the responses to both saturating and non-saturating GABA concentrations were slower than in the case of the long-term cultures. We conclude that the GABAa receptors in cultured neurons undergo profound kinetic changes involving the modulation of the binding reaction and transitions between bound states.

Słowa kluczowe

EN

receptor activation; non-equilibrium; hippocampal neuron; patch clamp; change; kinetics; hippocampus; cell culture; gamma-aminobutyric acid

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2004
• Tom: 09
• Numer: 1
• Opis fizyczny: p.61-67,fig.,ref.

Twórcy

autor

Mozrzymas J W

• Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, Poland

autor

Barberis A.

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