Genotyping of recombinant Pichia pastoris strains

• Autorzy: Viader-Salvado J M , Cab-Barrera E.L. , Galan-Wong L.J. , Guerrero-Olazaran M.
• Języki publikacji: EN

Abstrakty

EN

A simplified amplified-fragment length polymorphism (AFLP) method was used to genotype Pichia pastoris strains obtained by transformation of P. pastoris strain GS115 with a single integration vector. A total of 14 transformants and 3 control strains were analyzed, which generated 16 different band patterns. A clonal variation was obtained after the transformation process due to genetic differences generated during the transformation event of the host strain. Furthermore, the cluster analysis showed that the transformants with lesser genetic differences with respect to the P. pastoris host strain are the recombinant strains with the highest level of recombinant protein production.

Słowa kluczowe

EN

genotype; Pichia pastoris; clonal variation; transformation process; amplified fragment length polymorphism

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2006
• Tom: 11
• Numer: 3
• Opis fizyczny: p.348-359,fig.,ref.

Twórcy

autor

Viader-Salvado J M

• Universidad Autonoma de Nuevo Leon, Ave. Pedro de Alba s/n, Col.Ciudad Universitaria, 66450 San Nicolas de los Garza, N.L., Mexico

autor

Cab-Barrera E.L.

autor

Galan-Wong L.J.

autor

Guerrero-Olazaran M.

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