Interaction of the Pisum sativum nuclear matrix proteins with SAR DNA

• Autorzy: Rzepecki R. , Markiewicz E. , Adamiec R. , Szopa J.
• Języki publikacji: EN

Abstrakty

EN

We have isolated the nuclear matrices from Pisum sativum cell nuclei using three methods: i. standard procedure involving extraction of cell nuclei with 2 M NaCl and 1% Triton X-100; ii. the same with pretreatment of cell nuclei with 0.5 mM CuS04 (stabilisation step); and iii. method including lithium diiodosalicylate extraction. We compared the polypeptide pattern and residual DNA content of the nuclear matrices isolated. The nuclear matrices displayed a specific endonuclease activity which was due to the presence of a 32 kDa protein. The isolated nuclear matrices bound spe­cifically the scaffold-attached (SAR) DNA derived from human p interferon gene, in the exogenous SAR binding assay. Using the DNA-protein binding blot assay we demonstrated the presence of two nuclear matrix proteins of 66 kDa and 62 kDa which bound specifically SAR DNA.

Słowa kluczowe

EN

scaffold attached region; Triton X-100; DNA binding; protein; nuclear matrix; beta-interferon gene; lithium diiodosalicylate; binding protein; endonuclease; Pisum sativum

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1995
• Tom: 42
• Numer: 1
• Opis fizyczny: p.75-81,fig.

Twórcy

autor

Rzepecki R.

• Wroclaw University, S.Przybyszewskiego 63-77, 51-148 Wroclaw, Poland

autor

Markiewicz E.

autor

Adamiec R.

autor

Szopa J.

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