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2000 | 50 | 2 |

Tytuł artykułu

Pratylenchus brzeskii sp.nov. [Nematoda: Pratylenchidae], a root-lesion nematode from European coastal dunes

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
A root-lesion nematode, Pratylenchus brzeskii sp. nov., is described and illustrated from the roots of Ammophila arenaria (L.) Link from the coastal dunes of the Netherlands; this new species is morphologically characterized by; relatively long females (625-735 µm) with two lip annuli, long stylet (± 19 µm ) with broad stylet knobs, anteriorly slightly indented, short DGO; pharyngeal gland lobe ranging from 65 to 100 µm; four lateral lines present, inner lines closely together; spermatheca faint, oval to rectangular shaped; vulva posterior, ranging from 75 to 78 %, lips slightly protruding, posterior uterine sac 19 to 35 pm long; tail conoid, terminus smooth, rounded to narrowly rounded, relatively long hyaline tail part present. Males occurred abundantly and are slightly smaller in most morphological characteristics. PCR was used to amplify the internal transcribed spacer regions of a ribosomal DNA-cluster of P. brzeskii sp. nov. and compared with the morphological related species P. coffeae, P. loosi and P. penetrans. The rDNA fragments were cut with restriction enzymes; interspecific RFLP’s were observed. Pratylenchus brzeskii sp. nov. was also isolated from the coastal dune grasses Elymus farctus (Viv.) Meldris and Leymus arenarias Hochst., and detected in coastal dunes of Belgium, France and Poland.

Wydawca

-

Czasopismo

Rocznik

Tom

50

Numer

2

Opis fizyczny

p.255-261,fig.

Twórcy

autor
  • Nematology Section, Plant Protection Service, P.O.Box 9102, 6700 HC Waheningen, The Netherlands
  • Agricultural Research Centre, Burg. Van Gansberghelaan 96, 9820 Merelbeke, Belgium
autor
  • Agricultural Research Centre, Burg. Van Gansberghelaan 96, 9820 Merelbeke, Belgium

Bibliografia

  • Brzeski, M. W. 1998. Nematodes of Tylenchina in Poland and temperate Europe. Muzeum i Instytut Zoologii Polska Akademia Nauk, Warszawa, 397 pp.
  • Courtney, W. D., Polley, D. and V L. Miller. 1955. TAF, an improved fixative in nematode technique. Plant Disease Reporter, 39: 570-571.
  • Duncan, L. W, Inserra, R. N., Thomas, W. K., Dunn, D., Mustika, I., Frisse, L. M., Mendes, M. L., Morris, K. and D. T. Kaplan. 1999. Molecular and morphological analysis of isolates of Pratylenchus coffeae and closely related species. Nematropica, 29: 61-80.
  • Inserra, R. N., Duncan, L. W., Vovlas, N. and P. A. A. Loof. 1996. Pratylenchus loosi from pasture grasses in central Florida. Nematologica, 42:159-172.
  • Joyce, S. A., Reid, A., Driver, F. and J. Curran. 1994. Application of polymerase chain reaction (PCR) methods to the identification of entopathogenic nematodes, pp. 178-187. In: A. M. Burnell, R. U. Ehlers and J. P. Masson (eds.). Genetics of entomopathogenic nematode-bacterium complexes. European Commission report EUR 15681 EN, Luxembourg.
  • Karssen, G. and T. van Hoenselaar. 1998. Revision of the genus Meloidogyne Göldi, 1892 (Nematoda: Heteroderidae) in Europe. Nematologica, 44: 713-788.
  • Loot, P. A. A. 1991. The family Pratylenchidae. pp. 363-421. In: W. R. Nickle (ed.). Manual of agricultural nematology. Marcel Dekker, Inc., New York.
  • Seinhorst, J. W. 1959. A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica, 4: 67-69.
  • van der Putten, W H., van Dijk, C. and B. A. M. Peters. 1993. Plant-specific soil diseasis contribute to succesion in foredunes vegetation. Nature, 362: 53-55 Waeyenberge, L., Ryss, A. Moens, M., Pinochet, J. and T. C. Vrain. 2000. Molecular characterisation of Y&Pratylenchus species using rDNA Restriction Fragment Length Polymorphism. Nematology, 2: (in print).

Typ dokumentu

Bibliografia

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