Cloning and expression of two DNA fregments of the I-18 C region of Chironomus tentans. I. The scheme of the performed experiments and the applied technology

• Autorzy: Borowicz B P
• Języki publikacji: EN

Abstrakty

EN

The I-18 C region of the polytenic chromosome of Chironomus tentans contains the I-18 C gene. Two DNA fragments, reflecting two different open reading frames (i.e.ORF I and II) of two different transcripts (i.e. 1.8 and 4.6 kb RNA) of the I-18 C gene were isolated, then were cloned into the intermediate vector and next to the final vector in order to translate them in T7 RNA polymerase / promoter system. The translation of the ORF I and II was performed to obtain the polypeptides of these ORFs for further study. Here, the scheme of the performed experiments and the applied technology that were necessary to realize the goal of this research, are presented.

PL

Region i-18 C chromosomu politenicznego Chironomus tentans zawiera gen I-18 C. Dwa fragmenty DNA, odzwierciedlające dwie różne otwarte ramy odczytu (tj. ORF I i ORF II) dwóch różnych transkryptów (tj. 1.8 i 4.6 kpz RNA) genu I-18 C, zostały wyizolowane i następnie wklonowane do wektora pośredniego, a potem do wektora końcowego, w celu ich translacji w systemie promotora T7 RNA polimerazy. Translacja ORF I i ORF II była wykonana w celu uzyskania polipeptydów określonych przez te ORF do dalszych badań. W niniejszej pracy przedstawiono schemat wykonanych eksperymentów i zastosowaną technologię, która była potrzebna do zrealizowania celu badań.

Słowa kluczowe

EN

promoter system; cloning; I-18 C gene; T7 RNA polymerase; Chironomus tentans; polymerase chain reaction; expression; pET vector; DNA fragment

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 1998
• Tom: 38
• Numer: 1
• Opis fizyczny: p.30-41

Twórcy

autor

Borowicz B P

• Institute of Plant Protection, Miczurina 20, 60-318 Poznan, Poland

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