Alteration of O-specific polysaccharide structure of symbiotically defective Mesorhizobium loti mutant 2213.1 derived from strain NZP2213

• Autorzy: Turska-Szewczuk A. , Pietras H. , Borucki W. , Russa R.
• Języki publikacji: EN

Abstrakty

EN

Mesorhizobium loti mutant 2213.1 derived from the wild-type strain NZP2213 by Tn5 mutagenesis showed impaired effectiveness of symbiosis with the host plant Lotus corniculatus (Turska-Szewczuk et al., 2007 Microbiol Res, in press). The inability of lipopolysaccharide (LPS) isolated from the mutant 2213.1 strain or de-O-acetylated LPS of the parental cells to inactivate phage A1 particles implicated alterations in the LPS structure. The O-specific polysaccharide of the mutant was studied by chemical analyses along with  1H and  13C NMR spectroscopy, which clearly confirmed alterations in the O-chain structure. 2D NMR data showed that the mutant O-polysaccharide consists of a tetrasaccharide repeating unit containing non-substituted as well as O-acetylated or O-methylated 6-deoxytalopyranose residues. Additionally, an immunogold assay revealed a reduced number of gold particles on the mutant bacteroid cell surface, which could result from both a diminished amount of an O-antigenic determinant in mutant LPS and modifications of structural epitopes caused by alterations in O-acetylation or O-methylation of sugar residues. Western immunoblot assay of alkaline de-O-acetylated lipophilic M. loti NZP2213 LPS showed no reactivity with homologous serum indicating a role of O-acetyl groups in its O-specificity.

Słowa kluczowe

EN

6-deoxytalose; rhizobacteria; symbiosis; O-specific polysaccharide; Mesorhizobium loti; leguminous plant; mutant; host plant; symbiotic interaction; bacterial polysaccharide; Lotus corniculatus; polysaccharide structure; lipopolysaccharide

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2008
• Tom: 55
• Numer: 1
• Opis fizyczny: p.191-199,fig.,ref.

Twórcy

autor

Turska-Szewczuk A.

• Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland

autor

Pietras H.

autor

Borucki W.

autor

Russa R.

Bibliografia

• D’Antuono AL, Casabuono A, Couto A, Ugalde RA, Lepek VC (2005) Nodule development induced by Mesorhizobium loti mutant strains affected in polysaccharide synthesis. Mol Plant-Microbe Interact 18: 446–457.
• Apicella MA, Griffiss JM, Schneider H (1994) Isolation and characterization of lipopolysaccharides, lipooligosaccharides, and lipid A. Methods Enzymol 235: 242–252.
• Biosca EG, Oliver JD, Amaro C (1996) Phenotypic characterization of Vibrio vulnificus biotype 2 a lipopolysaccharidebased homogenous serogroup within Vibrio vulnificus. Appl Environ Microbiol 62: 918–927.
• Brett PJ, Burtnick MN, Woods DE (2003) The wbiA locus is required for 2-O-acetylation of lipopolysaccharides expressed by Burkholderia pseudomallei and Burkholderia thailandensis. FEMS Microbiol Lett 218: 323–328.
• Campbell GRO, Rheus BL, Walker GC (2002) Chronic intracellular infection of alfalfa nodules by Sinorhizobium meliloti requires correct lipopolysaccharide core. Proc Natl Acad Sci USA 99: 3938–3943.
• Choma A (2002) Lipopolysaccharides from Mesorhizobium huakuii and Mesorhizobium ciceri: chemical and immunochemical comparative data. Acta Biochim Polon 49:1043–1052.
• Choma A, Sowiński P, Mayer H (2000) Structure of the O-specific polysaccharide of Mesorhizobium huakuii IFO15243T. Carbohydr Res 329: 459–464.
• Forsberg LS, Noel KD, Box J, Russel WC (2003) Genetic locus and structural characterization of the biochemical defect in the O-antigenic polysaccharide of the symbiotically deficient Rhizobium etli mutant, CE166: replacement of N-acetylquinovosamine with its hexosyl-4- ulose precursor. J Biol Chem 278: 51347–51359.
• Gerwig GJ, Kamerling JP, Vliegenthart JF (1978) Determination of the D and L configuration of neutral monosaccharides by high-resolution capillary G.L.C. Carbohydr Res 62: 349–357.
• Hakomori S (1964) A rapid permethylation of glycolipid and polysaccharide catalyzed by methylsulfinyl carbanion in dimethyl sulfoxide. J Biochem (Tokyo) 55: 205–208.
• Janczarek M, Król J, Kutkowska J, Mazur A, Wielbo J, Borucki W, Kopcińska J, Łotocka B, Urbanik-Sypniewska T, Skorupska A (2001) Mutation in the pssB-pssA intergenic region of Rhizobium leguminosarum bv. Trifolii affects the surface polysaccharide synthesis and nitrogen fixation ability. J Plant Physiol 158: 1565–1574.
• Jarvis BDW, Pankhurst CE, Patel JJ (1982) Rhizobium loti, a new species of legume root nodule bacteria. Int J Syst Bacteriol 32: 378–380.
• Jarvis BDW, van Berkum P, Chen WX, Nour SM, Fernandez MP, Cleyet-Marel JC, Gillis M (1997) Transfer of Rhizobium loti, Rhizobium huakuii, Rhizobium ciceri, Rhizobium mediterraneum, and Rhizobium tianshanense to Mesorhizobium gen. nov. Int J Syst Bacteriol 47: 895–898.
• Kannenberg EL, Carlson RW (2001) Lipid A and O-chain modifications cause Rhizobium lipopolysaccharides to become hydrophobic during bacteroid development. Mol Microbiol 39: 379–391.
• Knirel YA, Paramonov NA, Shashkov AS, Kochetkov NK, Yarullin RG, Farber SM, Efremenko VI (1992) Structure of the polysaccharide chains of Pseudomonas pseudomallei lipopolysaccharides. Carbohydr Res 233: 185–193.
• Lam JS, Handelsman MYC, Chivers TR, MacDonald LA (1992) Monoclonal antibodies as probes to examine serotype- specific and cross-reactive epitopes of lipopolysaccharides from serotypes O2, O5, and O16 of Pseudomonas aeruginosa. J Bacteriol 174: 2178–2184.
• Maniatis T, Fritsch EF, Sambrook J (1982) In: Molecular cloning. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory.
• McNeil M, Darvill AG, Aman P, Franze´n LE, Albersheim P (1982) Structural analysis of complex carbohydrates using high-performance liquid chromatography, gas chromatography, and mass spectrometry. Methods Enzymol 83: 3–45.
• Noel KD, Forsberg LS, Carlson RW (2000) Varying the abundance of O antigen in Rhizobium etli and its effect on symbiosis with Phaseolus vulgaris. J Bacteriol 182: 5317–5324.
• Noel KD, Box JM, Bonne VJ (2004) 2-O-methylation of fucosyl residues of a rhizobial lipopolysaccharide is increased in response to host exudate and is eliminated in symbiotically defective mutant. Appl Environ Microbiol 70: 1537–1544.
• Rietschel ET, Gottert H, Lüderitz O, Westphal O (1972) Nature of linkages of the fatty acids present in the lipid- A component of Salmonella lipopolysaccharides. Eur J Biochem 28: 166–173.
• Russa R, Urbanik-Sypniewska T, Lindström K, Mayer H (1995a) Chemical characterization of two lipopolysaccharide species isolated from Rhizobium loti NZP2213. Arch Microbiol 163: 345–351.
• Russa R, Urbanik-Sypniewska T, Shashkov AS, Kochanowski H, Mayer H (1995b) The structure of the homopolymeric O-specific chain from the phenol soluble LPS of the Rhizobium loti type strain NZP2213. Carbohydr Polymers 27: 299–303.
• Russa R, Urbanik-Sypniewska T, Shashkov AS, Banaszek A, Zamojski A, Mayer H (1996) Partial structure of lipopolysaccharide isolated from Rhizobium leguminosarum bv. trifolii 24 and its GalA-negative Exo– mutant AR20. System Appl Microbiol 19: 1–8.
• Scott DB, Young CA, Collins-Emerson JM, Terzaghi EA, Rockman ES, Lewis PE, Pankhurst CE (1996) Novel and complex chromosomal arrangement of Rhizobium loti nodulation genes. Mol Plant-Microbe Interact 9: 187– 197.
• Soto MJ, Sanjuan J, Olivares J (2006) Rhizobia and plantpathogenic bacteria: common infection weapons. Microbiology 152: 3167–3174.
• Turska-Szewczuk A, Russa R (2000) A new Mesorhizobium loti HAMBI 1129 phage isolated from Polish soil. Curr Microbiol 40: 341–343.
• Turska-Szewczuk A, Łotocka B, Kutkowska J, Król J, Urbanik- Sypniewska T, Russa R (2007) The incomplete substitution of lipopolysaccharide with O-chain prevents the establishment of effective symbiosis between Mesorhizobium loti NZP2213.1 and Lotus corniculatus.
• Microbiol Res (in press doi:10.1016/j.micres.2006.11.011). Westphal O, Jann K (1965) Bacterial lipopolysaccharides. Extraction with phenol-water and further applications of the procedure. Meth Carbohydr Chem 5: 83–91.
• York WS, Darvill AG, McNeil M, Stevenson TT, Albersheim P (1986) Isolation and characterization of plant cell walls and cell wall components. Methods Enzymol 118: 3–40.
• Yang F-L, Lin L-P (1998) Cytostructure, lipopolysaccharides, and cell protein analysis from Rhizobium fredii. Bot Bull Acad Sin 39: 261–267.