Grapevine leaves as a source of natural antioxidants

• Autorzy: Amarowicz R. , Narolewska O. , Karamac M. , Kosinska A. , Weidner S.

Warianty tytułu

PL

Liscie winorosli jako zrodlo naturalnych przeciwutleniaczy

• Języki publikacji: EN

Abstrakty

EN

The crude extracts of phenolic compounds were obtained from grapevine leaves using 80% acetone and 80% methanol (v/v). The content of total phenolic and condensed tannins was determined using Folin & Ciocalteau’s phenol reagent, vanillin/HCl reagent, and protein precipitation method. The antioxidant properties of the extracts were investigated using the total antioxidant activity (TAA), DPPH radical scavenging activity and reducing power. The content of individual phenolic acids was determined using the HPLC method. The content of total phenolics in grapevine leaves and their extracts determined in this study was high (257 mg/g acetone extract and 232 mg/g methanolic extract). The content of condensed tannins in acetone extract was higher than in the methanolic one. Antiradical activity of both extracts against DPPH radical and reducing power were similar and strong. The acetone and methanolic extracts exhibited TAA of 1.37 and 1.44 mmol Trolox/g, respectively. Vitis vinifera leaves extracts were observed to contain gallic, caffeic, and p-coumaric acid. Gallic acid was a dominant phenolic acid. The majority of phenolic acids were found in the form of esters.

PL

Ekstrakt surowy związków fenolowych otrzymano z liści winorośli stosując 80% aceton i 80% metanol (v/v). Całkowitą zawartość fenoli oznaczono odczynnikiem fenolowym Folina i Ciocalteau, skondensowane taniny z odczynnikiem wanilina/HCl oraz poprzez precypitację białka. Właściwości przeciwutleniające ekstraktu badano stosując metodę całkowitej aktywności przeciwutleniającej (TAA), wymiatanie wolnego rodnika DPPH oraz zdolność redukcyjną. Zawartość fenoli ogółem w ekstraktach była wysoka i wynosiła 257 mg/g (ekstrakt acetonowy) oraz 232 mg/g (ekstrakt metanolowy). Zawartość skondensowanych tanin w ekstrakcie acetonowym była wyższa niż w metanolowym. Aktywność przeciwrodnikowa wobec rodnika DPPH, i zdolność redukcyjna były wysokie i nie zależały od składu ekstrakcyjnego. Ekstrakt acetonowy i metanolowy wykazywały TAA równe 1,37 i 1,44 mmol Trolox/ g. W ekstraktach z liści Vitis vinifera stwierdzono obecność kwasu galusowego, kawowego oraz p-kumarowego – dominował kwas galusowy. Większość fenolokwasów występowała w postaci estrów.

Słowa kluczowe

EN

antioxidant activity; phenolic compound; grape-vine leaf; phenolic acid; natural antioxidant

• Wydawca: -
• Czasopismo: Polish Journal of Food and Nutrition Sciences
• Rocznik: 2008
• Tom: 58
• Numer: 1
• Opis fizyczny: p.73-78,fig.,ref.

Twórcy

autor

Amarowicz R.

• Polish Academy of Sciences, Tuwima 10, P.O.Box 55, 10-747 Olsztyn, Poland

autor

Narolewska O.

autor

Karamac M.

autor

Kosinska A.

autor

Weidner S.

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