Molecular cloning and expression analysis of a new gene for short-chain dehydrogenase-reductase 9

• Autorzy: Liu S. , Huang C. , Li D. , Ren W. , Zhang H. , Qi M. , Li X. , Yu L.
• Języki publikacji: EN

Abstrakty

EN

We report here the cloning and characterization of a novel human short-chain dehydrogenases/ reductase gene SCDR9, isolated from a human liver cDNA library, and mapped to 4q22.1 by browsing the UCSC genomic database. SCDR9 containing an ORF with a length of 900 bp, encoding a protein with a signal peptide sequence and an adh_short domain. GFP localization shows SCDR9 protein concentrated in some site of the cytoplasm, but not in the ER. Expression pattern in eighteen tissues revealed that SCDR9 is expressed highly in liver. Soluble recombinant protein was successfully purified from Escherichia coli using pET28A(+) expression vector. Our data provides important information for further study of the function of the SCDR9 gene and its products.

Słowa kluczowe

EN

new gene; UCSC genomic database; gene; man; SCDR9 gene; cloning; isolation; human liver; liver; short-chain dehydrogenase-reductase superfamily; expression analysis

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2007
• Tom: 54
• Numer: 1
• Opis fizyczny: p.213-218,fig.,ref.

Twórcy

autor

Liu S.

• Fudan University, 220 Handan Road, Shanghai, 200433, P.R.China

autor

Huang C.

autor

Li D.

autor

Ren W.

autor

Zhang H.

autor

Qi M.

autor

Li X.

autor

Yu L.

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