Restriction analysis of capsid protein gene fragment of two Polish strains of European brown hare syndrome virus

• Autorzy: Chrobocinska M
• Języki publikacji: EN

Abstrakty

EN

Polish strains of EBHS virus isolated in 1992 (NP11/92) and in 1988 (L/98) were compared with standard Italian and French strains by restriction analysis of fragment coding the first part of capsid protein (VP60). The 726 bp fragment of capsid protein gene of strains tested was obtained by RT-PCR. The digestion of 726 bp fragments by BamH I endonuclease, revealed the same restriction profile for strain L/98 as Italian standard strain. These fragments of strain NP11/92 and French standard strain were not digested by BamH I. Endonuclease Sma I did not digested the amplified fragment of strains tested. The 726 bp fragment of strain L/98 demonstrated different restriction profiles for BsuR I and Hpa II enzymes in comparison with strain NP11/92 and standard strains. These results revealed differences in restriction profiles of fragment coding the first part of VP60 of Polish strains in comparison with standard strains and also between two Polish strains isolated in 1992 and 1998. These differences suggest the possibility of genotype changes of Polish EBHS strains isolated in different years.

Słowa kluczowe

EN

restriction analysis; infection; virus; Poland; European brown hare syndrome; capsid protein gene; population; hare

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2000
• Tom: 44
• Numer: 2
• Opis fizyczny: p.125-133,fig.

Twórcy

autor

Chrobocinska M

• National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

• 1. Asgari S., Hardy J.R.E., Cooke B.D.: Sequence analysis of rabbit haemorrhagic disease virus (RHDV) in Australia: alterations after its release. Arch. Virol. 1999, 144, 135-145.
• 2. Capucci L., Fallacara F., Grazioli S., Lavazza A., Pacciarini M.L., Brocchi E.: A further step in the evolution of rabbit hemorrhagic disease virus: the appearance of the first consistent antigenic variant. Virus Res. 1998, 58, 115-126.
• 3. Chrobocińska M.: ELISA and western blot tests used for the diagnosis of infection caused by EBHS virus in hares. Medycyna Wet. 1997, 53, 343-345.
• 4. Chrobocińska M.: Amplification of capsid gene of European brown hare syndrome virus by reverse transcriptase - polymerase chain reaction. Bull. Vet. Inst. Pulawy, 1998, 42, 125-133.
• 5. Chrobocińska M., Kozaczyński W., Mizak Z., Mizak B.: The case of infection of hare with European brown hare syndrome virus. Medycyna Wet. 1999, 55, 750-752.
• 6. Le Gall G., Huguet S., Vende P., Vautherot J.F., Rasschaert D.: European brown hare syndrome virus: molecular cloning and sequencing of the genome. J. Gen. Virol. 1996, 77, 1693-1697.
• 7. Le Gall G., Arnauld C., Boilletot E., Morisse J.P., Rasschaert D.: Molecular epidemiology of rabbit haemorrhagic disease virus outbreaks in France during 1988 to 1995. J. Gen. Virol. 1998, 79, 11-16.
• 8. Novotny N., Ros Bascunana C., Ballagi-Pordany A., Gavier-Widen D., Uhlen M., Belak S.: Phylogenetic analysis of rabbit haemorrhagic disease and European brown hare syndrome viruses by comparison of sequences from the capsid protein gene. Arch. Virol. 1997, 142, 657-673.
• 9. Schirrmeier H., Reimann I., Kollner B., Granzow H.: Pathogenic, antigenic and molecular properties of rabbit haemorrhagic disease virus (RHDV) isolated from vaccinated rabbits: detection and characterization of antigenic variants. Arch. Virol. 1999, 144, 719-735.
• 10. Wirblich C., Meyers G., Ohlinger V.F., Capucci L., Eskens U., Haas B., Thiel H.J.: European brown hare syndrome virus: relationship to rabbit hemorrhagic disease virus and other caliciviruses. J. Virol. 1994, 68, 5164-5173.