Influence of 5-aminoisoquinolin-1-one (5-AIQ) on neutrophil chemiluminescence in rats with transient and prolonged focal cerebral ischaemia and after reperfusion

• Autorzy: Hendryk S. , Czuba Z.P. , Jedrzejowska-Szypulka H. , Szliszka E. , Phillips V.A. , Threadgill M.D. , Krol W.
• Języki publikacji: EN

Abstrakty

EN

Stimulation of neutrophils by different factors increases their oxidative activity and the free radicals produced can report on the degree of activation. Poly(adenosine 5’-diphosphate ribose)polymerase-1 (PARP-1), a nuclear enzyme activated by strand breaks in DNA, plays an important role in the tissue injury associated with ischaemia-reperfusion injury and inflammation. 5-Aminoisoquinolin-1-one (5-AIQ) is a potent inhibitor of PARP-1 activity in vitro and in vivo in rats. Acute (80 min) and prolonged (24h) focal cerebral ischaemia was induced in rats by obstruction of the median cerebral artery, with or without reperfusion, with or without administration of 5-AIQ. The oxidative activity of neutrophils was measured by chemiluminescence. Administration of 5-AIQ.HCl (3.0 mg kg-1 b.w. - i.v.) caused a significant decrease in the oxidative activity of neutrophils in the group which had experienced chronic ischaemia for 24h but had no significant effect in the group which had received 80 min ischaemia, when compared to the control group. Increase of the oxidative activity of neutrophils was confirmed in rats with prolonged cerebral ischaemia, followed by reperfusion. 5-AIQ probably may decrease this activity through inhibition of PARP-1 in focus of local ischaemia as well as hence lowering the expression of inflammatory mediators by activated neutrophils.

Słowa kluczowe

EN

5-aminoisoquinolin-1-one; cerebral ischaemia-reperfusion; chemiluminescence; free radical; oxidative activity; rat; neutrophil

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2008
• Tom: 59
• Numer: 4
• Opis fizyczny: p.811-822,fig.,ref.

Twórcy

autor

Hendryk S.

• Medical University of Silesia, Jordana 19, 41-808 Zabrze-Rokitnica, Poland

autor

Czuba Z.P.

autor

Jedrzejowska-Szypulka H.

autor

Szliszka E.

autor

Phillips V.A.

autor

Threadgill M.D.

autor

Krol W.