Use of HIV as a gene transfer vector

• Autorzy: Pluta K. , Kacprzak M.M.
• Języki publikacji: EN

Abstrakty

EN

 Despite the extensive research efforts over the past 25 years that have focused on HIV, there is still no cure for AIDS. However, tremendous progress in the understanding of the structure and biology of the HIV virus led to the development of safe and potent HIV-based transgene delivery vectors. These genetic vehicles are referred to as lentiviral vectors. They appear to be better suited for particular applications, such as transgene delivery into stem cells, compared to other viral- and non-viral vectors. This is because Lentivirus-based vectors can efficiently infect nondividing and slowly dividing cells. In the present review article, the current state of understanding of HIV-1 is discussed and the main characteristics that had an impact on vector design are outlined. A historical view on the vector concept is presented to facilitate discussion of recent results in vector engineering in a broader context. Subsequently, a state of the art overview concerning vector construction and vector production is given. This review also touches upon the subject of lentiviral vector safety and related topics that can be helpful in addressing this issue are discussed. Finally, examples of Lentivirus-based gene delivery systems and their applications are presented, with emphasis on animal transgenesis and human gene therapy.

Słowa kluczowe

EN

virus-host interaction; nuclear import; vector improvement; transgenesis; life cycle; human disease; non-viral vector; HIV; transgene expression; AIDS; HIV virus; stem cell; reverse transcription; gene transfer vector; gene therapy; host defense; Lentivirus-based vector; genetic information; cell engineering; viral vector; HIV-1 based lentiviral vector; virology

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2009
• Tom: 56
• Numer: 4
• Opis fizyczny: p.531-595,fig.,ref.

Twórcy

autor

Pluta K.

• Polish Academy of Sciences, Trojdena 4, 02-109 Warsaw, Poland

autor

Kacprzak M.M.

Bibliografia

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