The interactions of phthalocyanines with stimulated and resting human peripheral blood mononuclear cells

• Autorzy: Frackowiak D. , Waszkowiak A. , Manikowski H. , Ion R.M. , Cofta J. , Wiktorowicz K.
• Języki publikacji: EN

Abstrakty

EN

The interactions of two metal-free phthalocyanines [(H2Pc) and Solar Pc (with four peripherical groups: SO2N(CH2CH2OH)2)] and of one metal substituted dye (CoPc) with resting and stimulated human peripheral blood mononuclear cells (PBMC) were com­pared. The absorption, fluorescence, photoacoustic and EPR spectra of both resting cells and cells stimulated by phytohaemagglutinin, incubated in dimethyl sulfoxide (DMSO) with very low or 95% water content and with or without dye addition, were measured. The fate of the light absorbed by the samples was investigated. It is known that singlet oxygen pro­duction is crucial for photodynamic action of dyes. Thermal deactivation and lumines­cence emission compete with this process, so investigation of these alternative paths of sensitizer deactivation provides information about photodynamic action. The incorpora­tion of the investigated dyes into cells and the perturbation of the cell structure caused by the dyes, the incubation solvent and the activator were investigated by comparing the spectral properties of PBMC before and after stimulation and incubation. Incubation of the cells for 1 h in a solution of Solar Pc in 99.5% aqueous DMSO, resulted in an efficient dye incorporation which was highly selective. Solar Pc being introduced much more effi­ciently into stimulated cells than into resting cells.

Słowa kluczowe

EN

peripheral blood; phthalocyanine; man; interaction; mononuclear cell; photodynamic therapy

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2001
• Tom: 48
• Numer: 1
• Opis fizyczny: p.257-269,fig.

Twórcy

autor

Frackowiak D.

• Poznan University of Technology, Nieszawska 13 A, 60-965 Poznan, Poland

autor

Waszkowiak A.

autor

Manikowski H.

autor

Ion R.M.

autor

Cofta J.

autor

Wiktorowicz K.

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