Subtle differences in glycosylation of blood group M and N type glycophorin A detected with anti-Tn lectins and confirmed by chemical analysis

• Autorzy: Krotkiewski H. , Duk M. , Lisowska E.
• Języki publikacji: EN

Abstrakty

EN

A higher content of Tn and sialyl-Tn receptors in glycophorin A of blood group N than in that of blood group M was suggested by reactions with anti-Tn lectins. Analysis of [^-elimination products of two blood group M and two blood group N preparations by gas liquid chromatography-mass spectrometry showed that GalNAc- -ol was detectable in minor amounts in all analyzed samples and its content was higher in the products obtained from desialylated antigens. Moreover, the content of Gal N Ac- -ol detected in blood group N samples was almost twice as high as in respective blood group M samples. Since blood group M and N antigens differ in two amino-acid residues, our results support the existence of sequence-dependent differences in efficiency of substitution of glycophorin GalNAc-Ser/Thr residues with galactose.

Słowa kluczowe

EN

lectin; beta-elimination; glycosylation; Tn receptor; glycophorin A; blood group

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1995
• Tom: 42
• Numer: 1
• Opis fizyczny: p.41-44,fig.

Twórcy

autor

Krotkiewski H.

• Polish Academy of Sciences, Czerska 12, 53-114 Wroclaw, Poland

autor

Duk M.

autor

Lisowska E.

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