Isolation and preliminary sequence characterization of beta-amylase gene promoters in rye [Secale cereale L.]

• Autorzy: Sadowski J , Rorat T , Cooke R , Delseny M
• Języki publikacji: EN

Abstrakty

EN

The isolation of rye ß-Amy1 and ß-Amy2 gene promoters from nuclear DNA using the inverse polymerase chain reaction (IPCR) technique and characterization of their sequences are presented. The conservation of ß-amylasc coding sequences allowed for simultaneous IPCR amplification of two different promoters with primers designed on the basis of the single known cDNA sequence. Two ß-amylasc gene promoters display a low sequence similarity (47%). Beside consensus TATA and CCAAT boxes, other sequence motives common to both promoters were found. In addition, the homology of amino acid sequences of plant ß-amylases available in the database is discussed.

Słowa kluczowe

EN

beta-amylase; rye; gene promoter; homology; amino acid; DNA cloning; prolamin; Secale cereale; mRNA; embryogenesis; endosperm; cDNA; inverse polymerase chain reaction

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 1997
• Tom: 38
• Numer: 3
• Opis fizyczny: p.241-251,fig.

Twórcy

autor

Sadowski J

• Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland

autor

Rorat T

• Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland

autor

Cooke R

• Laboratoire de Physiologie et Biologie Moleculaire des Plantes, Unite de Recherche Associee au Centre National de la Recherche Scientifique No. 565, Universite de Perpignan, France

autor

Delseny M

• Laboratoire de Physiologie et Biologie Moleculaire des Plantes, Unite de Recherche Associee au Centre National de la Recherche Scientifique No. 565, Universite de Perpignan, France

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