Czestosc koniugacyjnego transferu genow lekoopornosci z ESBL-dodatnich paleczek Enterobacteriaceae izolowanych od chorych z oddzialow pediatrycznych

• Autorzy: Franiczek R. , Dolna I. , Krzyzanowska B. , Szufnarowski K. , Kowalska-Krochmal B. , Zielinska M.

Warianty tytułu

EN

Conjugative transfer frequency of resistance genes from ESBL-producing Enterobacteriaceae strains isolated from patients hospitalized in pediatric wards

• Języki publikacji: PL

Abstrakty

PL

Badania przeprowadzono na 54 szczepach pałeczek z rodziny Enterobacte- riaceae wytwarzających ß-laktamazy typu ESBL, izolowanych od dzieci hospitalizowanych w Akademickim Szpitalu Klinicznym we Wrocławiu. Spośród badanych szczepów 47 (87%) przekazywało plazmidy kodujące ESBL do szczepu biorcy Escherichia coli K12 C600 z częstością 10-5-10-1. Wszystkie kliniczne izolaty oraz uzyskane transkoniuganty były wrażliwe in vitro na karbapenemy oraz norfloksacynę. Stwierdzono wysoki odsetek szczepów opornych na kotrimoksazol (94,4%), gentamicynę (94,4%) i amikacynę (85,2%). Oporność na aminoglikozydy oraz kotrimoksazol była często przekazywana w procesie koniugacji ze szczepów dawców do szczepu biorcy.

EN

The aim of this study was to evaluate the transfer frequency of plasmids encoding extended-spectrum ß-lactamases (ESBLs) from clinical isolates of Enterobacteriaceae to E. coli K12 C600 recipient strain. Additionally, resistance patterns to antimicrobial drugs of the isolates as well as transconjugants were analyzed. Fifty-four clinical strains belonging to the Enterobacteriaceae family were isolated from children hospitalized in Medical University Hospital in Wroclaw. All the strains studied were identified in automatic ATB system using ID32E tests. Besides, they were ESBL-positive as was confirmed by the double-disc synergy test (DDST). The minimal inhibitory concentration (MIC) was determined for twelve selected antibiotics and chemotherapeutics. The majority of the strains (87%) were able to transfer plasmid-mediated ESBL to E. coli K12 C600 recipient strain with a frequencies ranged from 10-5 to 10-1 per donor cell. All the isolates studied as well as their transconjugants were susceptible to imipenem, meropenem and norfloxacin (MIC <1mg/L). On the other hand, these strains displayed high level of resistance (MIC 512 - >1024 mg/L) to cefotaxime, ceftriaxone, gentamycin, amikacin and cotrimoxazole. Genetic markers conferring resistance to aminoglycosides and cotrimoxazole were often co-transferred to recipient strain in conjugation process.

Słowa kluczowe

PL

transfer genow; lekoopornosc; Enterobacteriaceae; ludzie chorzy; dzieci; opornosc na antybiotyki; izolaty; kotrimoksazol; gentamycyna; amikacyna; beta-laktamaza

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2006
• Tom: 58
• Numer: 1
• Opis fizyczny: s.41-51,tab.,bibliogr.

Twórcy

autor

Franiczek R.

• Akademia Medyczna we Wroclawiu, ul.Chalubinskiego 4, 50-368 Wroclaw

autor

Dolna I.

autor

Krzyzanowska B.

autor

Szufnarowski K.

autor

Kowalska-Krochmal B.

autor

Zielinska M.

Bibliografia

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