Badanie obecnosci bialka MPB 70 w podszczepie BCG uzywanym do produkcji polskiej szczepionki przeciwgruzliczej

• Autorzy: Janaszek W. , Wysokinska T. , Aleksandrowicz J.
• Języki publikacji: PL

Abstrakty

PL

Celem pracy było sprawdzenie czy podszczep BCG Moreau używany w Polsce do produkcji szczepionki przeciwgruźliczej produkuje i uwalnia do podłoża hodowlanego białko MPB 70 oraz czy białko to można zastosować jako reagent w teście skórnym do wykrywania uczulenia po szczepieniu BCG. Możliwość wykorzystania białka MBP 70 w celach diagnostycznych jest dyskutowana.

EN

The aim of the study was examination of MPB70 protein production by different BCG substrains and testing this protein as reagent in alergenic skin test on BCG vaccinated guinea pigs. Three BCG substrains: Danish 1331 (D), Japanese 172 (J) and Polish BCG Moreau (P) were used for the study. The protein MPB70 received from dr Nagai from Osaka University was used as reference preparation. It has been shown that MPB70 protein of molecular weight about 21 500 Da was present only in BCG Moreau (P) and Japanese (J) substrains. In immunoblotting test monoclonal antibodies reacted additionally with 43 000 Da protein in all tested substrains. We suspect that the detected additional molecule was a dimer of MPB70 protein. The development of skin reactions to MPB70 protein was seen in guinea pigs vaccinated with J and P substrains. The peak of alergy to MPB70 protein was observed 9 weeks after vaccination. In sera of vaccinated animals antibodies against MPB70 protein were detected by ELISA method. The possibility of using MPB70 protein for diagnostic purpose is discussed.

Słowa kluczowe

PL

podszczep BCG Moreau; szczepionki przeciwgruzlicze; Mycobacterium; podloza hodowlane; Polska; produkcja; bialko MPB 70; antygeny bialkowe

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 1998
• Tom: 50
• Numer: 1-2
• Opis fizyczny: s.47-54,fot.,tab.,bibliogr.

Twórcy

autor

Janaszek W.

• Panstwowy Zaklad Higieny, 00-791 Warszawa, ul.Chocimska 24

autor

Wysokinska T.

autor

Aleksandrowicz J.

Bibliografia

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