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2006 | 58 | 1 |

Tytuł artykułu

Proteolityczne i hemolityczne wlasciwosci paleczek z rodzaju Serratia

Warianty tytułu

EN
Haemolytic and proteolytic properties of Serratia spp. rods

Języki publikacji

PL

Abstrakty

PL
Zdolność wytwarzania hemolizyn i proteaz może mieć znaczenie w patogenezie zakażeń o etiologii Serratia sp. W pracy wykazano, że bez względu na warunki hodowli wysoki odsetek szczepów rodzaju Serratia wytwarzał enzymy proteolityczne.
EN
For over fifty years Serratia spp. rods have increasingly been isolated from clinical specimens. The isolation has arisen mainly from hospitalised patients. The development of Serratia spp. infections is associated with predisposing factors of patients, occurrence in both natural settings and within the hospital environment and the virulence factors of bacteria. CDM medium with casein sodium salt, gelatine and Skim Milk were used to detect proteolytic properties. Over 95% of analysed strains produced gelatinase. Casein sodium salt was hydrolysed by 89,6% to 93,1% of strains depending on cultivation temperature. Skim Milk was hydrolysed by 78,5% to 84,7% of analysed Serratia spp. Strains. Haemolytic properties of Serratia spp. rods were checked on a solid medium with human and sheep red blood cells at 22,30, and 37°C. The haemolysis of human erythrocytes at 37°C was detected most frequently at 38,2%. There were no significant differences identified when cultivation temperature was observed.

Wydawca

-

Rocznik

Tom

58

Numer

1

Opis fizyczny

s.53-58,rys.,bibliogr.

Twórcy

autor
  • Collegium Medicum im.L.Rydygiera w Bydgoszczy Uniwersytetu Mikolaja Kopernika w Toruniu, ul.M.Sklodowskiej-Curie, 85-094 Bydgoszcz

Bibliografia

  • 1. Braun V, Günther H, Neus B, Tautz C. Hemolytic activity of Serratia marcescens. Arch Microbiol 1985; 141: 371-6.
  • 2. Gavalieri SJ, Bohach GA, Snyder IS. Escherichia coli a-hemolysin: characteristics and probable role in pathogenicity. Microbiol Rev 1984; 48: 326-43.
  • 3. Gołuszko P. Ekspresja czynników wirulencji oraz lekoopornoœci u wariantów morfologicznych dysocjujących szczepów Serratia marcescens. Praca habilitacyjna, Akademia Medyczna w Bydgoszczy, 1996.
  • 4. Grimont PAD, Grimont F, Dulong de Rosnay HLC. Characterization of Serratia marcescens, S. liquefaciens and S. marinorubra by electrophoresis of their proteinases. J Gen Microbiol 1977; 99: 301-10.
  • 5. Grimont PAD, Grimont F, Le Minor S i inni. Comparatible results obtained from biotyping and serotyping in Serratia marcescens. J Clin Microbiol 1979; 10: 425-32.
  • 6. Hejazi A, Falkiner F. Serratia marcescens. J Med Microbiol 1997; 46: 903-12.
  • 7. König W, Faltin Y, Scheffer J i inni. Role of cell-bound hemolysin as a pathogenicity factor for Serratia infections. Infect Immunol 1987; 55: 2554-61.
  • 8. Maeda H, Molla A. Pathogenic potentials of bacterial proteases. Clin Chim Acta 1989; 185: 357-67.
  • 9. Marre R, Hacker J, Braun V. The cell-bound hemolysin of Serratia marcescens contributes to uropathogenicity. Microb Pathogen 1989; 7: 153-6.
  • 10. Matsumoto K, Maeda H, Takata K i inni. Purification and characterization of four proteases from a clinical isolate of Serratia marcescens kums 3958. J Bacteriol 1984; 157: 225-32.
  • 11. McKellar RC, Cholette H. Effect of temperature shifts on extracellular proteinase-specific mRNA pools in Pseudomonasfluorescens B52. Appl Environ Microbiol 1987; 53: 1973-6.
  • 12. Molla A, Yamamoto T, Maeda H. Characterization of 73 kDa thiol protease from Serratia marcescens and its effect on plasma proteins. J Bacteriol 1988; 104: 616-21.
  • 13. Montanaro D, Grasso GM, Annino I i inni. Epidemiological and bacteriological investigation of Serratia marcescens epidemic in nursery and in neonatal intensive care unit. J Hyg Camb 1984; 93: 67-78.
  • 14. Poole K, Braun V. Influence of growth temperature and lipopolisaccharide on hemolytic activity of Serratia marcescens. J Bacteriol 1988; 170: 5146-52.
  • 15. Ruan Y, Braun V. Hemolysis as a marker for Serratia. Arch Microbiol 1990; 154: 221-5.
  • 16. Zaidi M, Sifuentez J, Bobdilla M i inni. Epidemic of Serratia marcescens bacteriemia and meningitis in a neonatal unit of Mexico City. Infect Control Hosp Epidemiol 1989; 10; 14-20.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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