Effects of Aronia melanocarpa polyphenols on oxidative metabolism and apoptosis of neutrophils from obese and non-obese individuals

• Autorzy: Zielinska-Przyjemska M , Olejnik A. , Dobrowolska-Zachwieja A. , Grajek W.

Warianty tytułu

PL

Wplyw soku z aronii [Aronia melanocarpa Elliot] na metabolizm tlenowy i apoptoze neutrofilow osob zdrowych i otylych

• Języki publikacji: EN

Abstrakty

EN

Reactive oxygen species are postulated to be involved in systemic inflammation and oxidative stress in patients with obesity. Activated polymorphonuclear neutrophils (PMNs) generate extremely high amounts of reactive oxygen species, but these are normally targeted at pathogens inside intracellular phagosomes. The same beneficial antimicrobial functions, if not controlled, contribute to the tissue damaging effects of inflammatory reactions. The evidence from conventional epidemiology strongly implies fruits and vegetables in protection against oxidative stress. In our study, the in vitro effects of Aronia melanocarpa juice on oxidative metabolism and apoptosis of neutrophils from obese and non-obese individuals has been investigated. We tested 15 obese patients (aged 45 ±9 years, women, BMI = 34 ±4.9 kg/m2). Nine healthy subjects (BMI = 22.2 ±1.6 kg/m2) were enrolled as controls. Neutrophils were isolated and oxidant production, in response to phorbol 12-myristate 13-acetate, was characterized by using luminol dependent chemiluminescence (CL) and flow cytometric dichlorofluorescin oxidation assay. Caspase-3 activity, a marker of apoptosis execution, in human neutrophils, measured by a cleavage of the fluorogenic substrate Ac-DEVD-AMC. Additional experiments to assess the direct toxic effect of the aronia polyphenols were also carried out. Neutrophils from obese individuals had a significantly higher H2O2 production and CL response compared to controls (p < 0.05). The oxidative metabolism of PMNs was decreased by aronia juice treatment in both of groups, obese and non-obese individuals. The caspase-3 activity depended on the time of aronia juice treatment and was markedly increased in phorboltreated cells incubated with polyphenols for 24 hours. This natural product exert beneficial effects in cells and may, therefore, be useful in the treatment of obesity disorders

PL

Stres oksydacyjny i odczyn zapalny odgrywają istotną rolę w patogenezie otyłości. Jednym z głównych źródeł reaktywnych form tlenu (RFT) w organizmie człowieka są pobudzone neutrofile (PMNs). Zachwianie równowagi pomiędzy produkcją RFT a ich detoksykacją przez ustrojowe systemy antyoksydacyjne prowadzi do stanów zapalnych związanych z infiltracją granulocytów oraz rozprzestrzenienia się reakcji utleniania komórkowych makrocząsteczek. Działania prewencyjne upatruje się między innymi w optymalnym wykorzystaniu potencjału antyoksydacyjnego niektórych nieodżywczych składników diety. Celem pracy była ocena in vitro wpływu soku z aronii (Aronia melanocarpa Elliot) na metabolizm tlenowy i aktywność kaspazy-3, marker efektorowej fazy apoptozy, neutrofilów osób zdrowych i otyłych. Badaniami objęto 15 pacjentek (w wieku 45 ±9, BMI = 34 ±4,9 kg/m2) rozpoczynających leczenie z powodu otyłości prostej. Grupę kontrolną tworzyły zdrowe dawczynie krwi (BMI = 22,2 ±1,6 kg/m2). Oceniano intensywność chemiluminescencji zależnej od luminolu PMNs aktywowanych estrami forbolu (PMA), stężenie nadtlenku wodoru metodą cytometrii przepływowej z zastosowaniem dioctanu 2’,7’-dichlorofluorescyny oraz aktywność kaspazy-3 metodą fluorymetryczną rozszczepienia Ac-DEVD-AMC. U pacjentek z otyłością, w porównaniu z osobami zdrowymi, stwierdzono statystycznie znamienne zwiększenie produkcji H2O2 i intensywności chemiluminescencji stymulowanych PMNs (p < 0,05). Badany preparat w stężeniach 10-50% v/v i poddany trawieniu jelitowemu w tzw. sztucznym przewodzie pokarmowym wykazywał silne właściwości antyoksydacyjne w grupie osób zdrowych i otyłych. Sok z aronii po 24-godzinnej hodowli indukował spontaniczną i stymulowaną PMA apoptozę neutrofili (p < 0,05). Zatem wykorzystanie właściwości antyoksydacyjnych i przeciwzapalnych aronii może przeciwdziałać powikłaniom współistniejącym z otyłością.

Słowa kluczowe

EN

non-obese person; oxidative metabolism; neutrophil; reactive oxygen species; polyphenol; apoptosis; obese person; obesity; Aronia melanocarpa

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Technologia Alimentaria
• Rocznik: 2007
• Tom: 06
• Numer: 3
• Opis fizyczny: p.75-87,fig.,ref.

Twórcy

autor

Zielinska-Przyjemska M

• Poznan University of Medical Sciences, Swiecickiego 4, 60-781 Poznan, Poland

autor

Olejnik A.

autor

Dobrowolska-Zachwieja A.

autor

Grajek W.

Bibliografia

• Allen R.C., 1986. Phagocytic leukocyte oxygenation activities and chemiluminescence: a kinetic approach to analysis. Meth. Enzymol. 133, 449-493.
• Arroyo A., Modriansky M., Serinkan F.B., Bello R.I., Matsura T., Jiang J., Tyurin V.A., Tyurina Y.Y., Fadeel B., Kagan V.E., 2002. NADPH-oxidase-dependent oxidation and externalization of phosphatidylserine during apoptosis in Me2SO-differentiated HL-60 cells. J. Biol. Chem. 277, 49965-49975.
• Babior B.M., 2000. Phagocytes and oxidative stress. Am. J. Med. 109, 33-44.
• Bass D.A., Parce J.W., Dechatelet L.R., Szejda P., Seeds M.C., Thomas M., 1983. Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation. J. Immunol. 130, 1910-1917.
• Bruno A., Conus S., Ine's Schmid, Simon H.U., 2005. Apoptotic pathways are inhibited by leptin receptor activation in neutrophils. J. Immunol. 174, 8090-8096.
• Chan J.C., Cheung J.C., Stchouwer C.D., Emeis J.J., Tong P.C., Ko G.T., Yudkin J.S., 2002. The central role of obesity-associated dyslipidaemia, endothelial activation and cytokines in the metabolic syndrome-An analysis by structural equation modeling. Int. J. Obes. Relat. Metab. Disord. 26, 994-1008.
• Chen P.N., Chu S.C., Chiou H.L., Chiang C.L., Yang S.F., Hsieh Y.S., 2005. Cyanidin 3- glucoside and peonidin 3-glucoside inhibit tumor cell growth and induce apoptosis in vitro and suppress tumor growth in vivo. Nutr. Cancer. 53, 232-243.
• Cope M.B., Allison D.B., 2006. Obesity: person and population. Obesity 14, 156S-159S.
• Cotelle N., 2001. Role of flavonoids in oxidative stress. Curr. Topics Med. Chem. 1, 560-590.
• Dandona P., Mohanty P., Ghanim H., Aljada A., Browne R., Hamouda W., Prabhala A., Afzal A., Garg R., 2001. The suppressive effect of dietary restriction and weight loss in the obese on the generation of reactive oxygen species by leukocytes, lipid peroxidation, and protein carbonylation. J. Clin. Endocrinol. Metab. 86, 355-362.
• Edwards S.W., 1996. The O2- Generating NADPH oxidase of phagocytes: structure and methods of detection. Methods 9, 563-577.
• Fadeel B., Ahlin A., Henter J.I., Orrenius S., Hampton M.B., 1998. Involvement of caspasesin neutrophil apoptosis: regulation by reactive oxygen species. Blood 92, 4808-4818.
• Hampton M.B., Fadeel B., Orrenius S., 1998. Redox regulation of the caspases during apoptosis. Ann. New York Acad. Sci. 854, 323-335.
• Hertog M.G., Kromhout D., Aravanis C., Blackburn H., Buzina R., Fidanza F., Giampaoli S., Jansen A., Menotti A., Nedeljkovic S., Pekkarinen M., Simic B.S., Toshima H., Feskens E.J., Hollman P.C., Katan M.B., 1995. Flavonoid intake and long-term risk of coronary heart disease and cancer in the seven countries study. Arch. Intern. Med. 155, 381-386.
• Higdon J.V., Frei B., 2003. Obesity and oxidative stress. A direct link to CVD? Arterioscler. Thromb. Vasc. Biol. 23, 365-367.
• Hou D.X., Ose T., Lin S., Harazoro K., Imamura I., Kubo M., Uto T., Terahara N., Yoshimoto M., Fujii M., 2003. Anthocyanidins induce apoptosis in human promyelocytic leukemia cells:structure-activity relationship and mechanisms involved. Int. J. Oncol. 23, 705-712.
• Hou D.X., Yanagita T., Uto T., Masuzaki S., Fujii M., 2005. Anthocyanidins inhibit cyclooxy- genase-2 expression in LPS-evoked macrophages:structure-activity relationship and molecular mechanisms involved. Biochem. Pharmacol. 70, 417-425.
• Howell A.B., 2002. Cranberry proanthocyanins and the maintenance of urinary tract health. Crit. Rev. Food Sci. Nutr. 42, 273-278.
• Hung H.C., Joshipura K.J., Jiang R., Hu F.B., Hunter D., Smith-Warner S.A., Colditz G.A., Rosner B., Spiegelman D., Willett W.C., 2004. Fruit and vegetable intake and risk of major chronic disease. J. Natl. Cancer Inst. 96, 1577-1584.
• Kahkonen M.P., Hopia A.I., Heinonen M., 2001. Berry phenolics and their antioxidant activity. J. Agric. Food Chem. 49, 4076-4082.
• Keaney J.F., Jr., Larson M.G., Vasan R.S., Wilson P.W.F., Lipinska I.L., Corey D., Massaro J.M., Sutherland P., Vita J.A., Benjamin E.J., 2003. Obesity and systemic oxidative stress. Clinical correlates of oxidative stress in the Farmingham study. Arterioscler. Thromb. Vasc. Biol. 23, 434-439.
• Kowalczyk E., Krzesiński P., Kura M., Szmigiel B., Błaszczyk J., 2003. Anthycyanins in medicine. Pol. J. Pharmacol. 55, 699-702.
• Lazze M.C., Savio M., Pizzala R., Cazzalini O., Perucca P., Scovassi A.I., Stivala L.A., Bianchi L., 2004. Anthocyanins induce cell cycle perturbations and apoptosis in different human cell lines. Carcinogenesis 25, 1427-1433.
• Lin L.C., Kuo Y.C., Chou C.J., 2002. Immunomodulatory proanthocyanins from Ecdysanthera utilis. J. Nat. Prod. 65, 505-508.
• Lowry O.H., Rosenbrough N.J., Farr A.L., Randall R.J., 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275.
• Maianski N.A., Maianski A.N., Kuijpers T.W., Roos D., 2004. Apoptosis of neutrophils. Acta Haematol. 111, 56-66.
• Matsui T., Ebuchi S., Kobayashi M., Fukui K., Sugita K., Terahara N., Matsumoto K., 2002. Antihyperglycemic effect od diacylated anthocyanin derived Ipomoea batatas cultivar Ayamurasaki can be achieved through the alpha-glucosidase inhibitory action. J. Agric. Food Chem. 50, 7244-7248.
• Matsumoto M., Hara H., Chiji H., Kasai T., 2004. Gastroprotective effect of red pigments in black chokeberry fruit (Aronia melanocarpa Elliot) on acute gastric hemorrhagic lesions in rats. J. Agric. Food Chem. 52, 2226-2229.
• Middleton E. Jr., 1998. Effect of plant flavonoids on immune and inflammatory cell function. Adv. Exp. Med. Biol. 439, 175-182.
• Middleton E. Jr., Kandaswami C., Theoharides T.C., 2000. The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacol. Rev. 52, 673-751.
• Morrow J.D., 2003. Is oxidant stress a connection between obesity and atherosclerosis? Arterioscler. Thromb. Vasc. Biol. 23, 368-370.
• Nathan C., 2002. Points of control in inflammation. Nature 420, 846-852.
• Ohgami K., Ilieva I., Shiratori K., Koyama Y., Jin X.-H., Yoshida K., Kase S., Kitaichi N., Suzuki Y., Tanaka T., Ohno S., 2005. Anti-inflammatory effects of Aronia extract on rat endotoxininduced uveitis. Invest. Oph. Vis. Sci. 46, 275-281.
• Omenn G.S., 1995. What accounts for the association of vegetables and fruits with lower incidence of cancers and coronary heart disease? Ann. Epidemiol. 5, 333-335.
• Oszmiański J., Wojdyło A., 2005. Aronia melanocarpa phenolics and their antioxidant activity. Eur. Food Res. Technol. 1, 1-5.
• Robak J., Gryglewski R.J., 1996. Bioactivity of flavonoids. Pol. J. Pharmacol. 48, 555-564.
• Seeram N.P., Adams L.S., Zhang Y., Lee R., Sand D., Scheuller H.S., Heber D., 2006. Blackberry, black raspberry, blueberry, cranberry, red raspberry, and strawberry extracts inhibit growth and stimulate apoptosis of human cancer cells in vitro. J. Agric. Food Chem. 54, 9329-9339.
• Serhan C.N., Savill J., 2005. Resolution of inflammation: the beginning programs the end. Nature Immunol. 6, 1191-1197.
• Shih P.H., Yeh C.T., Yen G.C., 2005. Effects of anthocyanidin on the inhibition of proliferation and induction of apoptosis in human gastric adenocarcinoma cells. Food Chem. Toxicol. 43, 1557-1566.
• Tauber A.I., Fay J.R., Marletta M.A., 1984. Flavonoid inhibition of the human neutrophil NADPH-oxidase. Biochem. Pharmacol. 33, 1367-1369.
• Tordera M., Ferrandiz M.L., Alcaraz M.J., 1994. Influence of anti-inflammatory flavonoids on degranulation and arachidonic acid release in rat neutrophils. Z. Naturforsch. 49, 235-240.
• Vaughan A.T., Betti C.J., Villalobos M.J., 2002. Surviving apoptosis. Apoptosis 7, 173-177.
• Wawer I., 2005. Aronia - polski paradoks [Aronia - Polish paradox]. Agropharm, Tuszyn [in Polish].
• Witko-Sarsat V., Rieu P., Descamps-Latscha B., Lesavre P., Halbwachs-Mecarelli L., 2000. Neutrophils: molecules, functions and pathophysiological aspects. Lab. Invest. 80, 617-653.
• Yasui K., Kobayashi N., Yamazaki T., Agematsu K., Matsuzaki S., Ito S., Nakata S., Baba A., Koike K., 2005. Superoxide dismutase (SOD) as a potential inhibitory mediator of inflammation via neutrophil apoptosis. Free Radic. Res. 39, 755-762.
• Yeh C.T., Yen G.C., 2005. Induction of apoptosis by the Anthocyanidins through regulation of Bcl-2 gene and activation of c-Jun N-terminal kinase cascade in hepatoma cells. J. Agric. Food Chem. 53, 1740-1749.
• Zarkesh-Esfahani H., Pockley A.G., Wu Z., Hellewell P.G., Weetman A.P., Ross R.J., 2004. Leptin indirectly activates human neutrophils via induction of TNF-a. J. Immunol. 172, 1809-1814.
• Zielińska-Przyjemska M., Wiktorowicz K., 2006. An in vitro study of the protective effect of the flavonoid silydianin against reactive oxygen species. Phytother. Res. 20, 115-119.