Value of the IgG avidity in the diagnosis of recent toxoplasmosis: A comparative study of four commercially available anti-Toxoplasma gondii IgG avidity assays

• Autorzy: Horvath K N , Szenasi Z. , Danka J. , Kucsera I.
• Języki publikacji: EN

Abstrakty

EN

The determination of the anti-Toxoplasma immunoglobulin G (IgG) avidity is a diagnostic method which has been intensively used for differentiating of recent and more distant Toxoplasma gondii infection in a single serum sample. Finding of low avidity anti-Toxoplasma IgG antibodies usually indicates recently acquired infection, while the presence of high avidity IgG antibodies contradicts to recently acquired infection. Recently, a number of commercial kits have been introduced for determining the avidity of anti-Toxoplasma IgG antibodies. Therefore, it seemed advisable to make comparative studies in order to investigate the correspondences and discrepancies in the results obtained by the different kits. We compared and analysed the results obtained with four commercially available anti-Toxoplasma IgG avidity assays. They were expected to give well correlated results, but some differences between the kits were observed in the ranking of anti-Toxoplasma IgG avidity ("high" or "low" or "borderline"). Among others, differences in the dilution method or differences in the type, quality or quantity of the Toxoplasma antigen used in the different assays might explain the observed divergences.

Słowa kluczowe

EN

toxoplasmosis; serology; congenital toxoplasmosis; immunoglobulin G; Toxoplasma gondii

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2005
• Tom: 50
• Numer: 3
• Opis fizyczny: p.255-260,ref.

Twórcy

autor

Horvath K N

• Johan Bela National Center for Epidemiology, Gyali ut 2-6 H-1097 Budapest, Hungary

autor

Szenasi Z.

autor

Danka J.

autor

Kucsera I.

Bibliografia

• Alvarado-Esquivel C., Sethi S., Janitschke K., Hahn H., Liesenfeld O. 2002. Comparison of two commercially available avidity tests for Toxoplasma-specific IgG antibodies. Archives of Medical Research, 33, 520-523.
• Barberi A., Gistri A., Cappelletti F., Giordano I. 2001. Diagnostic value of IgG avidity in Toxoplasma infection: comparison of 3 commercial kits. Journal of Infectious Diseases, 184, 944.
• Bobic B., Sibalic D., Djurkovic-Djakovic O. 1991. High levels of IgM antibodies specific for Toxoplasma gondii in pregnancy 12 years after primary Toxoplasma infection. Gynecologic and Obstetric Investigation, 31, 182-184.
• Cozon G.J., Ferrandiz J., Nebhi H., Wallon M., Peyron F. 1998. Estimation of the avidity of immunoglobulin G for routine diagnosis of chronic Toxoplasma gondii infection in pregnant women. European Journal of Clinical Microbiology and Infectious Diseases, 17, 32-36.
• Del Beno V., Canessa A., Bruzzi P., Fiorelli M.A., Terragna A. 1989. Significance of specific immunoglobulin M in the chronological diagnosis of 38 cases of toxoplasmic lymphadenopathy. Journal of Clinical Microbiology, 27, 2133-2135.
• Gergely J. 1998. Immunbiológia. Medicina, Budapest.
• Gutiérrez J., Rodríguez M., Piédrola G., del Carmen Maroto M. 1997. Detection of IgAand low-avidity IgG antibodies for the diagnosis of recent active toxoplasmosis. Clinical Microbiology and Infection, 3, 658-662.
• Hedman K., Lappalainen M., Seppala I., Makela O. 1989. Recent primary Toxoplasma infection indicated by a low avidity of specific IgG. Journal of Infectious Diseases, 159, 736-739.
• Hedman K., Lappalainen M., Söderlund M., Hedman M. 1993. Avidity of IgG in serodiagnosis of infectious diseases. Reviews in Medical Microbiology, 4, 123-129.
• Holliman R., Raymond R., Renton N., Johnson J. 1994. The diagnosis of toxoplasmosis using IgG avidity. Epidemiology and Infection, 112, 399-408.
• Jenum P.A., Stray-Pedersen B., Gundersen A.-G. 1997. Improved diagnosis of primary Toxoplasma gondii infection in early pregnancy by determination of antitoxoplasma immunoglobulin G activity. Journal of Clinical Microbiology, 35, 1972-1977.
• Lappalainen M., Koskela P., Koskiniemi M., Ämmälä P., Hiilesmaa V., Teramo K., Raivio K. O., Remington J.S., Hedman K. 1993. Toxoplasmosis acquired during pregnancy: improved serodiagnosis based on avidity of IgG. Journal of Infectious Diseases, 167, 691-697.
• Liesenfeld O., Montoya J.G., Kinney S., Press C., Remington J.S. 2001. Effect of testing for IgG avidity in the diagnosis of Toxoplasma gondii infection in pregnant women: experience in a US reference laboratory. Journal of Infectious Diseases, 183, 1248-1253.
• Montoya J.G., Liesenfeld O., Kinney S., Press C., Remington J.S. 2002. VIDAS test for avidity of Toxoplasma-specific immunoglobulin G for confirmatory testing of pregnant women. Journal of Clinical Microbiology, 40, 2504-2508.
• Pelloux H., Bran E., Vernet G., Marcillat S., Jolivet M., Guergour D., Fricker-Hidalgo H., Goullier-Fleuret A., Ambrosie Thomas P. 1998. Determination of anti-Toxoplasma gondii immunoglobulin G avidity: adaptation to the VIDAS system (Bio- Mérieux). Diagnostic Microbiology and Infectious Disease, 32, 69-73.
• Petersen E., Pollak A., Reiter-Owona I. 2001. Recent trends in research on congenital toxoplasmosis. International Journal for Parasitology, 31, 115-144.
• Remington J.S., Klein J.O. 1995. Infectious diseases of the fetus and newborn infant. WB Saunders, Philadelphia.
• Sensini A., Pascoli S., Marchetti D., Castronari D., Marangi M., Sbaraglia G., Cimmino C., Favero A., Castelletto M., Mottola A. 1996. IgG avidity in the serodiagnosis of acute Toxoplasma gondii infection: a multicenter study. Clinical Microbiology and Infection, 2, 25-29.
• Szénási Z., Ozsvár Z., Nagy E., Jeszenszky M., Szabó J., Gellén J., Végh M., Verhofstede C. 1997. Prevention of congenital toxoplasmosis in Szeged, Hungary. International Journal of Epidemiology, 26, 428-435.
• Szénási Z., Mecseki R., Lukács K, Urban E., Rákos K., Ozsvár Z., Nagy E. 1996/1997. Analysis of the serological results of Toxoplasma screening of pregnant women in Szeged with special regard to the year 1995. Parasitologica Hungarica, 29/30, 17-26.