Zasiedlenie kostrzewy trzcinowej [Festuca arundinacea Schreb.] przez Neotyphodium coenophialum oraz jego wplyw na rozwoj wybranych mikroorganizmow in vitro

• Autorzy: Panka D

Warianty tytułu

EN

Infestation of tall fescue [Festuca arundinacea Schreb.] with Neotyphodium coenophialum and its influence on growth of chosen microorganisms in vitro

• Języki publikacji: PL

Abstrakty

PL

Badania przeprowadzono w celu określenia zasiedlenia uprawianych w Polsce odmian kostrzewy trzcinowej przez endofita - Neotyphodium coenophialum oraz określenie jego wpływu na wzrost wybranych mikroorganizmów in vitro. Przetestowano 17 prób nasion 11 odmian kostrzewy trzcinowej. Grzybnię endofita wykrywano metodą mikroskopową po uprzednim wybarwieniu różem bengalskim. Obecność N. coenophialum potwierdzano metodą PCR. Badanie wpływu endofita na wzrost wybranych 15 mikroorganizmów przeprowadzono w warunkach laboratoryjnych na płytkach Petriego z pożywką PDA w temperaturach: 10, 20 i 30°C. Endofita wykryto tylko w dwóch próbach nasion odmian Barrocco (42%) i Terros (2%). Żywą grzybnię N. coenophialum wyizolowano z roślin wyrosłych z zasiedlonych nasion odmiany Barrocco. W temperaturze 30°C zaobserwowano największy hamujący wpływ endofita na wzrost grzybni Bipolaris sorokiniana, Fusarium avenaceum, F. equiseti, Microdochium nivale oraz Gaeumannomyces graminis. W przypadku ostatniego patogena zanotowano największą strefę zahamowania wzrostu - 4 mm. Rozwój B. sorokiniana i M. nivale nie był hamowany w temp. 10°C, a F. avenaceum w temp. 10 i 20°C.

EN

Occurrence of Neotyphodium coenophialum in tall fescue cultivars cultivated in Poland and determination an endophyte inhibition effect on mycelium growth of chosen microorganisms in vitro were investigated. Seventeen seed lots of 11 cultivars of tall fescue were examined. The endophyte mycelium was dyed with bengal rose and microscopically examined to detect N. coenophialum. Occurrence of endophyte was checked with PCR method. Influence of endophyte on growth of 15 microorganisms was established in the laboratory conditions on Petri dishes with PDA medium at 10, 20 and 30°C. Neotyphodium coenophialum occurred only in two seed lots, ‘Barrocco’ - 42% and Terros - 2%. Living mycelium of endophyte was isolated only from ‘Barrocco’. The highest mycelium growth inhibition of Bipolaris sorokiniana, Fusarium avenaceum, F. equiseti, Microdochium nivale and Gaeumannomyces graminis by endophyte at 30°C was recorded. The highest width of growth inhibition zone (4mm) was detected for the last pathogen. Mycelium growth of B. sorokiniana and M. nivale was not inhibited at 10°C, and for F. avenaceum at 10 and 20°C.

Słowa kluczowe

PL

Neotyphodium coenophialum; zasiedlanie; kostrzewa trzcinowa; endofity; mikroorganizmy; wplyw nastepczy; Festuca arundinacea; badania in vitro

EN

Neotyphodium coenophialum; fungi colonizing grass; tall fescue; endophyte; microorganism; sequent impact; Festuca arundinacea; in vitro study

• Wydawca: -
• Czasopismo: Acta Agrobotanica
• Rocznik: 2005
• Tom: 58
• Numer: 2
• Opis fizyczny: s.369-379,fot.,tab.,bibliogr.

Twórcy

autor

Panka D

• Katedra Fitopatologii, Akademia Techniczno-Rolnicza, ul.Ks.A.Kordeckiego 20, 85-225 Bydgoszcz

Bibliografia

• Bacon C.W., Richardson M.D., White J.F., 1997. Modifications and uses of endophyte-enhanced turfgrasses - A role for molecular technology. Crop Sci. 37: 1415-1425.
• Cappelli C., Buonaurio R., 2001. Occurrence of endophytic fungi in grass seeds and plants in Italy. In: Paul V.H. and Dapprich P.D. (eds) Proceedings of the 4th International Neotyphodium/Grass Interactions Symposium, Soest, Gennany, 27-29 September 2000: 131-137.
• Clement S.L.,Youssef N.N., Bruehl G.W., Kaiser W.J., Elberson L.R., Bradley V., 2004. Effects of different storage temperatures on grass seed germination and Neotyphodium survival. In: Kallenbach, R., Rosenkrans, C. Jr., Lock, T.R. (eds), Proceedings of the 5th International Symposium on Neotyphodium/Grass Interactions, Fayetteville, AR, USA, May 23-26, 2004, #511: 163-165.
• Cross D. L., 2001. Toxic effects of Neotyphodium coenophialum in cattle and horses. In: Paul V.H. and Dapprich P.D. (eds) Proceedings of the 4th International Neotyphodium/Grass Interactions Symposium, Soest, Germany, 27-29 September 2000: 219-235.
• Dapprich P., Klose A., Paul V.H. 1994. A survey on the occurrence of endophytic fungi in European seed lots of Lolium perenne. IOBC/WPRS Bulletin 17(1): 131-137.
• Doss R.P., Welty R.E., 1995. A polymerase chain reaction-based procedure for detection of Acremonium coenophialum in tall fescue. Phytopathology, 85, 8: 913-917.
• Eggestein St., Pfannmöller M., Schöberlein W., 1996. Acremonium spp. - occurrence in cultivars and collected ecotypes of genus Festuca. IOBC/WPRS Bulletin, 19(7): 161-167.
• Faeth S.H., Sullivan T.J., Hamilton C.E., 2001. What maintains high levels of Neotyphodium endophytes in native grasses? A dissenting view and alternative hypotheses. In: Paul V.H. and Dapprich P.D. (eds) 4th International Neotyphodium/Grass Interactions Symposium, Soest, Germany, 27-29 September 2000: 65-69.
• Johnson M.C., Dahlmann D.L., Siegel M.R., Bush L.P., Latch G.C.M., Potter D.A., Varney D.R., 1985. Insect feeding deterrents in endophyte infected tall fescue. Plant Dis. 70: 380-382.
• Leuchtmann A., Clay K., 1990. Isozyme variation in Acremonium/Epichloë fungal endophyte complex. Phytopathology 80: 1133-1139.
• Lewis G. C., 1996a. A review of research on endophytic fungi worldwide, and its relevance to European grassland, pastures and turf. IOBC/WPRS Bulletin 19(7): 17-24.
• Lewis G.C., 1996b. Effect of cutting height on perennial ryegrass with and without infection with endophyte and ryegrass mosaic virus. IOBC/WPRS Bulletin 19(7): 55-58.
• Malinowski D.P., Belesky D.P., 2000. Adaptations of endophyte-infected cool-season grasses to environmental stresses: Mechanisms of drought and mineral stress tolerance. Crop Sci. 40: 923-940.
• Malinowski D.P., Belesky D.P., Lewis G.C., 2005. Abiotic stresses in endophytic grasses. In: Roberts C.A., West C.P., Spiers D.E. (eds), Neotyphodium in cool-season grasses. Blackwell Publ.: 187-199.
• Moyer J.L., 2004. Humidity reduces tall fescue seed-borne endophyte viability. In: Kallenbach, R., Rosenkrans, C. Jr., Lock, T.R. (eds), Proceedings of the 5th International Symposium on Neotyphodium/Grass Interactions, Fayetteville, AR, USA, May 23-26, 2004, #515: 172-174.
• Pańka D., Łukanowski A., 2001. Occurrence of Acremonium lolii in perennial ryegrass (Lolium perenne L.) cultivated in the Kujawy and Pomerania region of Poland. In: Paul V.H. and Dapprich P.D. (eds), Proceedings of the 4th International Neotyphodium/Grass Interactions Symposium, Soest, Germany, 27-29 September 2000: 419-421.
• Pańka D., Sadowski Cz., 2002. Occurrence of fungal endophytes in perennial ryegrass (Lolium perenne L.) cultivars in Poland. In: Durand, J.L., Emile, J.C., Huyghe, C., Lemaire, G. (eds), Multi-function Grasslands, Quality Forages, Animal Products and Landscapes. Grassl. Sci. Eur., 7: 540-541.
• Pańka, D., Podkówka, L., Lamparski, R., 2004. Preliminary observations on the resistance of meadow fescue (Festuca pratensis Huds.) infected by Neotyphodium uncinatum to diseases and pests and nutritive value. In: Kallenbach, R., Rosenkrans, C. Jr., Lock, T.R. (eds), Proceedings of the 5th International Symposium on Neotyphodium/Grass Interactions, Fayetteville, AR, USA, May 23-26, 2004, #401: 88-90.
• Pfannmöler M., Eggestein S., Schöberlein W., 1994. Endophytes in European varieties of Festuca species. IOBC/WPRS Bulletin 17(1): 105-109.
• Popay A.J., Bonos S.A., 2005. Biotic responses in endophytic grasses. In: Roberts C. A., West C.P., Spiers D.E. (eds), Neotyphodium in cool-season grasses. Blackwell Publis.: 163-185.
• Porter J.K., Thompson F.N. Jr, 1992. Effects of fescue toxicosis on reproduction in livestock. J. Anim. Sci. 70: 1594-1603.
• Riccioni L., Monopoli V., Odoardi M., 1994. Endophytic fungi in tall fescue in Italy. IOBC/WPRS Bulletin 17(1): 127-130.
• Rolston M.P., Hare M.D., Moore K.K., Christensen M.J., 1986. Viability of Lolium endophyte fungus in seed stored at different moisture contents and temperatures. New Zealand J. Exp. Agric., 14: 297-300.
• Saha D.C., Jackson M.A., Johnson-Cicalese J.M., 1988. A rapid staining method for detection of endophytic fungi in turf and forage grasses. Phytopathology 78 (2):237-239.
• Schmidt D., 1994. Du nouveau sur les endophytes de la fétuque des prés. Rev. Suisse Agric. 26: 57-63.
• Siegel M.R., Bush L.P., 1996. Defensive chemicals in grass-fungal endophyte associations. Recent Adv. Phytochem. 30: 81-118.
• Siegel M.R., Latch G.C.M., Johnson M.C., 1985. Acremonium fungal endophytes of tall fescue and perennial ryegrass: Significance and control. Plant Dis. 69(2): 179-183.
• Thompson F.N., Stuedeman J.A., 1993 Phytophysiology of fescue toxicosis. Agric. Ecosyst. Envir. 44: 263-281.
• Wäli P., 2001. Seed transmitted endophytic fungi in wild grass populations in Finland. In: Paul V.H. and Dapprich P.D. (eds), Proceedings of the 4th International Neotyphodium/Grass Interactions Symposium, Soest, Gennany, 27-29 September 2000: 93-96.
• West C. P., 1994. Physiology and drought tolerance of endophyte-infected grasses. In: Bacon C.W. & White Jr. J.F. (eds), Biotechnology of endophytic fungi of grasses. CRC Press: 87-99.
• Wheatley W.M., 2005. Endophytes, quality assurance, and the seed trade in eastern Australia. In: Roberts C.A., West C.P., Spiers D.E. (eds), Neotyphodium in cool-season grasses. Blackwell Publ.: 351-360.
• White Jr, J.F., Cole, G.T., 1985. fendophyte host associations in forage grasses III. In vitro inhibition of fungi by Acremonium coenophialum. Mycologia 77: 487—489.