An approach to cloning of Pi-b rice blast resistance gene

• Autorzy: Rybka K , Miyamoto M. , Nakamura S. , Kawasaki S.
• Języki publikacji: EN

Abstrakty

EN

A contig of clones from BAC rice genomic library encompassing blast resistance gene Pi-b was constructed. On an average eight clones (8 ± 2.6) were picked up by each marker, which was expected basing on the BAC library size (Nakamura et al. 1997). The 2.4 cM distance between flanking RFLP markers G 1234 and RZ 213 (Miyamoto et al. 1996) was spanned with 4 steps of contig including 25 clones. The physical distance of 370 kb between flanking markers corresponds to a small ratio of physical and genetical distances (155 kb/cM) due to a probable structure of the gene locus near the telomeric end of the chromosome. Markers cosegregating with blast resistance against Magnoporthe grisea were localized in a 2 kb restriction fragment. A new border marker was found on the telomeric side of the Pi-b gene, less than 10 kb from cosegregating markers. No clear marker for the centromeric side of the gene was found but the position of Pi-b rice blast resistant gene was narrowed to within at least 50 kb, which is to our knowledge the most precised estimation of the position of this gene.

Słowa kluczowe

EN

Magnoporthe grisea; cloning; blast; resistance gene; rice

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 1997
• Tom: 19
• Numer: 4
• Opis fizyczny: p.521-528,fig.

Twórcy

autor

Rybka K

• Plant Breeding and Acclimatization Institute-Radzikow, POB 1019, 00-950 Warsaw, Poland

autor

Miyamoto M.

autor

Nakamura S.

autor

Kawasaki S.

Bibliografia

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