Genetic variation in natural and cultivated populations of Primula veris

• Autorzy: Morozowska M. , Krzakowa M.
• Języki publikacji: EN

Abstrakty

EN

Primula veris, a well-known pharmaceutical plant, is a long-lived perennial protected by law in Poland, so its rhizomes and roots can be collected as pharmaceutical stock only from cultivation. Genetic variation of three natural P. veris populations numbering 500-1200 individuals and of three cultivated populations derived from seeds collected from natural sites was investigated in respect of two enzyme systems: phosphogluconate dehydrogenase (6PGD) and diaphorase (DIA). Four presumptive loci were identified from these two enzyme systems. In 6PGD, only one (6PGD-2) of two detected loci was polymorphic, consisting of three alleles a, b and c. Each of two electrophoretically detected loci in DIA was polymorphic and had two alleles. Comparison of the cultivated and natural populations revealed slight differentiation in the presence and composition of genotypes for 6PGD-2, while for DIA all populations except one preserved the same set of genotypes. Mean values of the polymorphism index for three loci ranged from 0.239 to 0.345 for natural populations and from 0.303 to 0.446 for cultivated populations, indicating that cultivated populations were more polymorphic than natural ones. The level of heterozygosity in the examined populations was very low. Mean values for Ho calculated for three polymorphic loci ranged from 0.033 to 0.056. The observed low heterozygosity level was confirmed by high values of Wright’s fixation index, ranging from 0.798 to 0.910.

Słowa kluczowe

EN

cultivation; Primulaceae; raw material; cowslip; natural population; electrophoresis; Primula veris; cultivated population; genetic diversity; plant; genetic variation

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2003
• Tom: 45
• Numer: 2
• Opis fizyczny: p.177-182,fig.

Twórcy

autor

Morozowska M.

• August Cieszkowski Agricultural University, Wojska Polskiego 71C, 60-625 Poznan, Poland

autor

Krzakowa M.

Bibliografia

• Antrobus S, and Lack AJ. 1993. Genetics of colonizing and established populations of Primula veris. Heredity 71: 252-258.
• Calis I, Yürüker A, Rüegger H, Wright AD, and Sticher O. 1992. Triterpene saponins from Primula veris subsp. macrocalyx and Primula elatior subsp. meyeri. Journal of Natural Products 55: 1299-1306.
• Hamrick JL, and Godt MJ. 1990. Allozyme diversity in plant species. In: Brown AHD, Clegg MT, Kahler AL, Weir BS [eds.], Plant population genetics, breeding and genetic resources, 43-63. Sinauer Associates Inc., MA.
• Hedrick PW. 1974. Genetic similarity and distance; comments and comparisons. Evolution 29: 362-366.
• HegnauerR. 1969. Chemotaxonomie der Pflanzen. Band 5, 389-393. Birkhäuser Verlag, Basel, Stuttgart.
• HegnauerR. 1990. Chemotaxonomie der Pflanzen. Band 9, 289-292. Birkhäuser Verlag, Basel, Boston, Berlin.
• Jain SK, and Workman PW. 1967. Generalized F-statistics and the theory of inbreeding and selection. Nature 214: 674-678.
• Kahler AL, Allard RW, Krzakowa M, Wehrhan CF, and Nevo E. 1980. Associations between isozyme phenotypes and environment in the slender wild oat (Avena barbara) in Israel. Theoretical and Applied Genetics 56: 31-47.
• Kéry M, Metthies D, and Spillman HH. 2000. Reduced fecundity and offspring performance in small populations of the declining grassland plants Primula veris and Gentiana lutea. Journal of Ecology 88: 17-30.
• Kohlmünzer S. 1993. Farmakognozja. PZWL, Warszawa.
• Morozowska M. 1999. Importance of studies on population biology for species protection of medicinal plants on an example of selected populations of cowslip (Primula veris L.). Roczniki Akademii Rolniczej w Poznaniu, CCCX, Melior. Inż. Śród. 20, pt. II: 437-445.
• Morozowska M. 2000. Charakterystyka ekologiczna naturalnych populacji Primula veris L. na Nizinie Wielkopolskiej. Roczniki Akademii Rolniczej w Poznaniu, CCCXXII, Botanika 3: 165-173.
• Morozowska M, and Krzakowa M. 2003. Genetic variation of cowslip (Primula veris L.) populations (West Poland). Acta Societatis Botanicorum Poloniae 72: 331-336.
• Morozowska M, and Urbański Ł. 2000. Charakterystyka kwitnienia i owocowania oraz ocena produkcyjności nasiennej roślin pierwiosnka lekarskiego (Primula veris L.) z populacji zlokalizowanych na Nizinie Wielkopolskiej. Roczniki Akademii Rolniczej w Poznaniu, CCCXXII, Botanika 3: 175-184.
• Nei M. 1972. Genetic distance between populations. American Naturalist 106: 283-292.
• Nei M. 1978. Molecular evolutionary genetics. Columbia University Press, New York.
• Neubauer N, and März RW. 1994. Placebo-controlled, randomised double-blind clinical trial with Sinupret sugar coated tablets on the basis of a therapy with antibiotics and decongestant nasal drops in acute sinusitis. Phytomedicine 1: 177-181.
• Sirkkomaa S. 1983. Calculations on the decrease of genetic variation due to the founder effect. Hereditas 99: 11-20.
• Strzelecka H, and Kowalski J. 2000. Encyklopedia zielarstwa i ziołolecznictwa. PWN, Warszawa.
• Tamm CO. 1972. Survival and flowering of perennial herbs III. The behaviour of Primula veris on permanent plots. Oikos 23: 159-166.
• Wedderburn F, and Richards AJ. 1990. Variation in within-morph incompatibility sites in heteromorphic Primula L. New Phytologist 116: 149-162.