The induction of cytochrome P450 isoform, CYP4A1, by clofibrate coincides with activation of ethanolamine-specific phospholipid base exchange reaction in rat liver microsomes

• Autorzy: Lenart J. , Komanska I. , Jasinska R. , Pikula S.
• Języki publikacji: EN

Abstrakty

EN

Administration of a hypolipidaemic drug, clofibrate, to rats resulted, 24 h after a single intraperitoneal injection (250 mg/kg body weight), in pronounced enhancement of the rate of phosphatidylethanolamine (PE) synthesis via the PE-specific base exchange (PEBE) reaction in liver microsomes. This was accompanied by 3.4-fold activation of microsomal ω-hydroxylation of lauric acid by cytochrome P450 4A1 isoform (CYP4A1) and an increase in the protein content of this isoform in endoplasmic reticulum (ER) membranes. Since PE represents a class of phospholipids (PL) prerequisite for proper functioning of CYP4A1, and the PEBE reaction is an inducible pathway of PL synthesis in hepatocytes under metabolic stress, one may speculate that this reaction is switched on when extensive remodelling of PL molecular species or/and massive synthesis of lipid bilayer components for membrane assembly is required.

Słowa kluczowe

EN

peroxisome proliferator; clofibrate; phosphatidylethanolamine; phospholipid synthesis; enzyme; lipid metabolism; liver; rat; microsome; cytochrome P450

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1998
• Tom: 45
• Numer: 1
• Opis fizyczny: p.119-126,fig.

Twórcy

autor

Lenart J.

• M.Nencki Institute of Experimental Biology, L.Pasteura 3, 02-093 Warsaw, Poland

autor

Komanska I.

autor

Jasinska R.

autor

Pikula S.

Bibliografia

• 1. Hardwick, J.P., Song, B.-J., Huberman, E. & Gonzales, F.J. (1987) Isolation, complemen­tary DNA sequence, and regulation of rat he­patic lauric acid ^-hydroxylase (cytochrome P- 450^). J. BioL Chem. 262, 801-810.
• 2. Bains, S.K., Gardiner, S.M., Mannweiler, K., Gillett, D. & Gibson, G.G. (1985) Immuno­chemical study on the contribution of hypo- lipidaemic-induced cytochrome P-452 to the metabolism of lauric acid and arachidonic acid. Biochem. Pharmacol. 34, 3221-3229.
• 3. Simpson, A.E.C.M., Brammar, W.J., Pratten, M.K. & Elcombe, C.R. (1995) Translactational induction of CYP4A expression in 10.5-day neonatal rats by the hypolipidemic drug clofi­brate. Biochem. Pharmacol. 50, 2021- 2032.
• 4. Gearing, K.L., Gottlicher, M., Widmark, E., Banner, C.D., Toilet, P., Stromstedt, M., Raf­ter, J.J., Berge, R.K. & Gustafsson, J.A. (1994) Fatty acid activation of the peroxisome prolif­erator activated receptor, a member of the nu­clear receptor gene superfamily. J. Nutr. 124 (suppl), 1284S-1288S.
• 5. Bayerl, T., Klose, G., Ruckpaul, K. & Schwarze, W. (1985) Interaction of hexane phosphonic acid diethyl ester with phospholip­ids in hepatic microsomes and reconstituted Olliposomes as studied by P-NMR. Biochim. Biophys. Acta 812, 437-446.
• 6. Milton, M.N., Elcombe, C.R. & Gibson, G.G. (1990) On the mechanism of induction of cyto­chrome P450IVA1 and peroxisome prolifera­tion in rat liver by Clofibrate. Biochem. Phar­macol. 40, 2727-2732.
• 7. Hogeboom, G.H. (1955) Fractionation of cell components of animal tissue. Methods Enzy- mol 1, 16-19.
• 8. Rakowska, M., Jasiriska, R., Lenart, J., Ko- manska, I., Makowski, P., Dygas, A. & Pikula, S. (1997) Membrane integrity and phos­pholipid movement influence the base ex­change reaction in rat liver microsomes. MoL Cell: Biochem. 168, 163-176.
• 9. Weinhold, P.A. & Rethy, V.B. (1974) The sepa­ration, purification, and characterization of ethanolamine kinase and choline kinase from rat liver. Biochemistry 13, 5135-5141.
• 10. McMaster, Ch.R. & Choy, P.C. (1992) Serine regulates phosphatidylethanolamine biosyn­thesis in the hamster heart. J. Biol. Chem. 267, 14586-14591.
• 11. Dygas, A. & Zborowski, J. (1989) Effect of Tri­ton X-100 on the activity and solubilization of rat liver mitochondrial phosphatidylserine de­carboxylase. Acta Biochim. Polon. 36, 131-141.
• 12. Bligh, E.G. & Dyer, WJ. (1959) A rapid me­thod of total lipid extraction and purification. Can. J. Biochem. 37, 911-917.
• 13. Parker, G.L. &Orton, T.C. (1980) Induction by oxyisobutyrates of hepatic and kidney micro­somal cytochrome P-450 with specificity to­wards hydroxylation of fatty acids; in Biochem­istry, Biophysics and Regulation of Cytochrome P-450 (Gustafsson, J.A., Carlstedt-Dulce, J., Mode, A. & Rafter, J., eds.) pp. 373-377, El- sevier/North-Holland Biomedical Press.
• 14. Lowry, O.H., Rosebrough, N.J., Farr, A.L. & Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275.
• 15. Touchstone, J.C., Chen, J.C. & Beaver, K.M. (1980) Improved separation of phospholipids in thin layer chromatography. Lipids 15, 61-62.
• 16. Rouser, G., Fleisher, S. & Yamamoto, A. (1970) Two dimensional thin layer chroma­tographic separation of polar lipids and deter­mination of phospholipids by phosphorus analysis of spots. Lipids 5, 494-496.
• 17. Kawashima, Y., Mizuguchi, H., Musoh, K. & Kozuka, H. (1994) The mechanism for the in­creased supply of phosphatidylcholine for the proliferation of biological membranes by clofi- bric acid, a peroxisome proliferator. Biochim. Biophys. Acta 1212, 311-318.
• 18. Lalwani, N.D., Alvares, K., Reddy, M.K., Reddy, M.N., Parikh, I. & Reddy, J.K. (1987) Peroxisome proliferator-binding protein: Iden­tification and partial characterization of nafe- nopin-, clofibric acid-, and ciprofibrate-binding proteins from rat liver. Proc. Natl Acad. Sci. U.S.A. 84, 5242-5246.
• 19. Bronfman, M., Amigo, L. & Morales, M.N. (1986) Activation of hypolipidaemic drugs to acyl-coenzyme A thioesters. Biochem. J. 239, 781-784.
• 20. Kozuka, H., Yamada, J., Horie, S., Watanabe, T., Suga, T. & Ikeda, T. (1991) Characteristics 125_of induction of peroxisomal fatty acid oxi­dation-related enzymes in rat liver by drugs. Biochem. Pharmacol. 41, 617-623.
• 21. Watanabe, T. & Suga, T. (1988) Suppression of clofibrate-induced peroxisome proliferation in rat liver by nicardipine, a calcium antago­nist. FEBS Lett. 232, 293-297.
• 22. Watanabe, T., Okawa, S., Itoga, H., Imanaka, T. & Suga, T. (1992) Involvement of calmodu­lin- and protein kinase C-related mechanism in an induction process of peroxisomal fatty acid oxidation-related enzymes by hypolipidemic peroxisome proliferators. Biochim. Biophys. Acta 1135, 84-90.
• 23. Shackleton, G.L., Gibson, G.G., Sharma, R.K., Howes, D., Orrenius, S. & Kass, G.E.N. (1995) Diverse mechanisms of calcium mobilisation by peroxisome proliferators in rat hepato- cytes. Toxicol Appl. Pharmacol. 130, 294- 303.
• 24. Alvarez, J., Montero, M. & Garcia-Sancho, J. (1991) Cytochrome P^50 may link intracellu- lar Ca" stores with plasma membrane Ca~ influx. Biochem. J. 274, 193-197.
• 25. Jasinska, R., Rakowska, M., Lenart, J., Ko­mariska, I. & Pikula, S. (1996) Nonenzyma- tically-evoked and cytochrome P450-depen- dent lipid peroxidation inhibits synthesis of phosphatidylethanolamine via the ethanola- mine base exchange reaction in rat liver micro­somes. FEBS Lett. 386, 33-38.
• 26. Makowski, P., Szewczyk, A., Jasiriska, R. & Pikula, S. (1997) An antagonist of ATP- regulated potassium channels, the guanidine derivative U-37883A, stimulates the synthesis of phosphatidylserine in rat liver endoplasmic reticulum membranes. FEBS Lett. 409, 292-296.
• 27. Lamberger, T., Desvergne, B. & Wahli, W. (1996) Peroxisome proliferator-activated re­ceptors: A nuclear receptor signaling pathway in lipid physiology. Annu. Rev. Cell Biol 12, 335-363.
• 28. Nebert, D.W. (1990) Growth signal pathways. Nature (London) 347, 709-710.
• 29. Kliewer, S.A., Umesono, K., Noonan, D.J., Heyman, R.A. & Evans, R.M. (1992) Conver­gence of 9-cis retinoic acid and peroxisome proliferator signalling pathways through het- erodimer fcrmation of their receptors. Nature (London) 358, 771-774.
• 30. Umesono, K., Murakami. K.K., Thompson, C.C. & Evans, R.M. (1991) Direct repeats as se­lective response elements for the thyroid hor­mone, retinoic acid, and vitamin-D3 receptors. Cell 65, 1255-1266.
• 31. Kliewer, S.A., Forman, B.M., Blumberg, B., Ong, E.S., Borgmeyer, U., Mangelsdorf, D.J., Umesono, K. & Evans, R.M. (1994) Differen­tial expression and activation of a family of murine peroxisome proliferator-activated re­ceptors. Proc. Natl. Acad. Sci. U.S.A. 91, 7355-7359.