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Tytuł artykułu

Wplyw warunkow hodowli na hydrofobowe wlasciwosci paleczek z rodzaju Serratia

Warianty tytułu

Języki publikacji

PL

Abstrakty

PL
Właściwości hydrofobowe drobnoustrojów są jednym z ważnych niespecyficznych czynników adhezji, która ma znaczenie w namnażaniu mikroorganizmów na stałych powierzchniach. W niniejszej pracy wykazano wpływ warunków hodowli na zmianę właściwości hydrofobowych powierzchni komórek pałeczek Serratia sp.
EN
The cell surface hydrophobicity (CSH) is a non-specific adhesion factor that is important in the proliferation of microorganisms on solid surfaces. Serratia spp. is a bacterium that has been increasingly implicated as a primary pathogen in numerous human infections, particularly in urinary tract infections. CSH of 60 Serratia spp. strains isolated from clinical materials was evaluated using the ammonium sulfate salt aggregation test. Bacteria were grown for 24h and 48 h at room temperature (22°C) and 37°C on enrichment broth and agar (Biomed), enrichment agar with 5% human blood and medium composed of agar granulated (Becton Dickinson), neopeptone (Difco) and 1% (v/v) glycerol. CSH was estimated most frequently when the analyzed strains in enrichment brolh were cultured. When grown in enrichment broth cells of Serratia spp. at room temperature were more hydrophobic (43% after 24 h and 47% after 48 h) than those at 37°C (30% after 24 h and 33% after 48 h). CSH of the examined Serratia spp. strains were depended on the temperature, time of the culture of bacteria and the kind of media. The influence of the culture conditions on the changes in CSH of the analyzed bacteria may suggest significance of these properties in the pathogenesis of Serratia spp.

Wydawca

-

Rocznik

Tom

52

Numer

1

Opis fizyczny

s.9-15,rys.,bibliogr.

Twórcy

autor
  • Akademia Medyczna, 85-094 Bydgoszcz, ul.M.Sklodowskiej-Curie 9
autor

Bibliografia

  • 1. Alwan A, Deignan T, O'Sullivan M i inni. Quantitative assay of Salmonella adherence to intestinal epithelial cells; a new method for assessing novel intervention products. J Microbiol Meth 1998; 33; 163-70.
  • 2. Bar-Ness R, Avrahamy N, Matsuyama T, Rosenberg M. Increased cell surface hydrophobicity of a Serratia marcescens NS 38 mutant wetting activity. J Bacteriol 1988; 170: 4361.-64.
  • 3. Bar-Mess R, Rosenberg M. Putative role of a 70 kDa outer-surface protein in promoting cell-surface hydrophobicity of Serratia marcescens RZ. J Gen Microbiol 1989; 135; 2277-81.
  • 4. Bruno DW. The relationship between autoaggregation, cell surface hydrophobicity and virulence of the fish pathogen Renibacterium salmoninarum. FEMS Microbiol Lett 1988; 51: 135.
  • 5. Ferreirós CM. Criado MT, Sainz V i inni. Changes in surface hydrophobicity and charge in Neisseria meningitidis and their correlation with the association to phagocytic cells. Rev esp Fisiol 1989; 45: 105-10.
  • 6. Gałuszko P, Nowacki M. Extracellular hemolytic activity of Serratia marcescens. FEMS Microbiol Lett 1989; 61: 207-12.
  • 7. Gospodarek E, Czajkowski H, Ulatowska B. Bacteria of Serratia genus as etiological factor of hospital infections. Med Sei Monit 1998; 4: 1024-29.
  • 8. Haddy RJ, Mann BL, Nadkanii DD i inni. Nosocomial infections in the community hospital: severe infection due to Serratia species. J Fam Pract 1996; 42: 273-77.
  • 9. Hazen KZ„ Plotkin BJ, Klimas DM. Influence of growth conditions on cell surface hydrophobic of Candida albicans and Candida glabrata. Infect Immun 1986; 54: 269-71.
  • 10. Klotz SA, Drutz DJ. Zajic JE. Factors governing adherence of Candida albicans to plastic surfaces. Infect Immun 1985; 50: 97-101.
  • 11. Lindahl M, Faris A, Wadström T, Hjérten S. A new tes based on „salting out" to measure relative surface hydrophobicity of bacterial cells. Biochim Riophys Acta 1981; 677: 471-76.
  • 12. Lu D, Boyd B, Lingwood CA. The expression and characterization of putative adhesin B from H. influenzae. FEMS Microbiol Lett 1998; 165: 129-37.
  • 13. McKenney D, Hübner J, Muller E i inni. The ica locus Staphylococcus epidermidis encodes production of the capsular polysaccharide/adhesin. Infect Immun 1998; 66: 4711-20.
  • 14. Pelletier CH, Boutey CH, Cayucía Ch i inni. Cell surface characteristics of Lactobacillus casei subsp. casei, Lactobacillus paracasei subsp. paracasci, and Lactobacillus rhamnosus strains. Appl Environ Microbiol 1997; 63: 1725-31.
  • 15. Rosenberg M, Kjelleberg S. Hydrophobic interactions: role in bacterial adhesion. Adv Microb Ecol 1986; 9: 353-93.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-becfed04-9f85-42cb-b97f-6cc83069d282
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