Crystallization and preliminary crystallographic studies of a new crystal form of papain from Carica papaya

• Autorzy: Kozak M. , Kozian E. , Grzonka Z. , Jaskolski M.
• Języki publikacji: EN

Abstrakty

EN

A new crystal form of papain from the latex of Carica papaya, complexed with an inhibitor (Z-Arg-Leu-Val-Gly-CHN2) was obtained by the vapor-diffusion method using a methanol/ethanol mixture as a precipitant. The slat-like crys­tals are monoclinic, space group P2 1, with unit cell parameters a = 42.6 A, b = 49.8 A, c = 50.5 A, β= 111.9°, and contain one molecule in the asymmetric unit. The crystals are stable in the X-ray beam and diffract beyond 1.8 A. A molecular model has been placed in the unit cell by molecular replacement.

Słowa kluczowe

EN

thiol protease; Carica papaya; crystallization; X-ray; protein crystallography; papain; cysteine protease; crystal form; latex; inhibitor

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1997
• Tom: 44
• Numer: 3
• Opis fizyczny: p.601-605,fig.

Twórcy

autor

Kozak M.

• A.Mickiewicz University, H.Wieniawskiego 1, 61-712 Poznan, Poland

autor

Kozian E.

autor

Grzonka Z.

autor

Jaskolski M.

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