Isolation and expression pattern of RGS21 gene, a novel RGS member

• Autorzy: Li X. , Chen L. , Ji C. , Liu B. , Gu J. , Xu J. , Zou X. , Gu S. , Mao Y.
• Języki publikacji: EN

Abstrakty

EN

Regulators of G-protein signaling (RGS) proteins are known for the RGS domain that is composed of a conserved stretch of 120 amino acids, which binds directly to activated G-protein α subunits and acts as a GTPase-activating protein (GAP), leading to their deactivation and termination of downstream signals. In this study, a novel human RGS cDNA (RGS21), 1795 bp long and encoding a 152-amino acid polypeptide, was isolated by large-scale sequencing analysis of a human fetal brain cDNA library. Unlike other RGS family members, RGS21 gene has no additional domain/motif and may represent the smallest known member of RGS family. It may belong to the B/R4 subfamily, which suggests that it may serve exclusively as a negative regulator of αi/o family members and/or αq/11. PCR analysis showed that RGS21 mRNA was expressed ubiquitously in the 16 tissues examined, implying general physiological roles.

Słowa kluczowe

EN

gene expression; regulator; GTPase-activating protein; RGS21 gene; G-protein signalling

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2005
• Tom: 52
• Numer: 4
• Opis fizyczny: p.943-946,fig.,ref.

Twórcy

autor

Li X.

• Fudan University, Shanghai, People's Republic of China

autor

Chen L.

autor

Ji C.

autor

Liu B.

autor

Gu J.

autor

Xu J.

autor

Zou X.

autor

Gu S.

autor

Mao Y.

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