Release of Pr3plus ions from liposomes affected by single and double-chain ammonium chlorides

• Autorzy: Gabrielska J. , Pawlowicz P. , Sarapuk J.
• Języki publikacji: EN

Abstrakty

EN

By using the lH—NMR method and paramagnetic ions Pr3+, the incorporation into the liposome membrane of a series of biologically active single-chain (TGE-n) and double-chain (DGE-n) quaternary ammonium salts was studied. Corresponding compounds of both groups differed in their alkyl chain-length (n = 8,10,12,14 and 16). Minimal concentration CA of compounds TGE-n and DGE-n which caused total release of Pr3+ ions from the external layer of liposome membrane and the ratio of CA to the 4.5 mM extravesicular concentration of Pr3+ (CPr) used in experiments was determined. Based on the results obtained, one can conclude that the effectiveness of Pr3+ release from liposome membrane is (with n = 12,14 and 16) twice as high for double-chain compounds as for analogous single-chain compounds, and about 2.5 and 5 times higher for DGE-10 and DGE-8 than for TGE-10 and TGE-8, respectively.

Słowa kluczowe

EN

liposome; double-chain ammonium chloride; quaternary ammonium salt; biological membrane

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 1992
• Tom: 01
• Numer: 2
• Opis fizyczny: p.9-12,fig.

Twórcy

autor

Gabrielska J.

• Agricultural University, Wroclaw, Poland

autor

Pawlowicz P.

autor

Sarapuk J.

Bibliografia

• RUCKA M, OŚWIĘCIMSKA M., WITEK S. New bio- cides for cooling water treatment. Part III. Quaternary ammonium salts derivative of glycine esters. Environment Protection Eng. 9, 25—30,1983.
• KLESZCZYŃSKA H., SARAPUK J., PRZESTALSKI S., WITEK S. The role of the alkyl chain in the interaction of glycine esters with erythrocyte and model erythrocyte lipid membranes. Studia Biophysica 116, 115—122,1986.
• SARAPUK J., PRZESTALSKI S., WITEK S, VUCELIC D. Effect of some quaternary ammonium salts on planar lecithin membranes. Studia Biophysica 100, 113—117, 1984.
• GABRIELSKA J., PRZESTALSKI S, OŚWIĘCIMSKA M. and WITEK S. The role of alkoxymethylene trimet- hylammonium chlorides in desorption of praseodymiumions from liposome membranes. Studia Biophysica 102, 5—13,1984.
• KUCZERA J., JANAS T„ PRZESTALSKI S., WITEK S., OŚWIĘCIMSKA M. Influence of some amphiphilic deri¬vatives of glycine esters on sulphate ion transport across lecithin liposome membrane. Studia Biophysica 105, 167—176,1985.
• PRZESTALSKI S., HENDRICH A., WITEK S. Influence of some amphiphilic glycine esters on phase transition of dipalmitoylphosphatidylcholine. Studia Biophysica 116, 19—25,1986.
• KLESZCZYŃSKA H„ SARAPUK J, PRZESTALSKI S., KILIAN M. Mechanical properties of red cell and BLM in the presence of some mono and bis-quatemary ammonium salts. Studia Biophysica 135, 191—199,1990.
• CERBON J. NMR evidence for the hydrophobic interac¬tion of local anesthetics. Biochim. Biophys. Acta 290, 51—57,1972.
• FERENDEZ M.S., CERBON J. The importance of the hydrophobic interactions of local anesthetic in the displacement of polyvalent cations from artificial lipid membranes. Biochim. Biophys. Acta 298, 8—14,1973. KASZUBA H, HUNT G. R. A. A 'H—NMR study of the influence of h-alcohols on the stoichiometry of melit- tin-induced permeability of phospholipid vesicles. Biochim. Biophys. Acta 985, 106—110,1989.
• HUNT G.R.A., JONES I.C. A ‘H—NMR investigation of the effects of ethanol and general anaestetics on ion channels and membrane fusion using unilamellar phosp¬holipid membranes. Biochim. Biophys. Acta 736, 1—10,1983.
• SINGLETON W. S., GRAY H. S, BROWN H. L., WHI¬TE J. L. Chromatographically homogeneous lecithin from egg phosphilipid. J. Am. Oil Chem. Soc. 42, 53—56,1965. BYSTROV V. F, DUBROVINA N. I., BARSUKOV L. I., BERGELSON L.D. NMR differentiation of the internal and external phospholipid membrane surfaces using paramagnetic Mn1+ and Eu3+ ions. Chem. Phys. Lipids 6, 343—350, 1971.
• HUNT G.R.A., JAWAHARLAL K. A lH—NMR investigation of the mechanism for the ionophore activity of the bile salts in phospholipid vesicular membranes and the effect of cholesterol. Biochim. Biophys. Acta 601, 678—684, 1980.
• HUNT G. R. A., TIPPING L.R.H., BELMONT M.R. Rate-determining processes in the transport of Pr3+ ions by the ionophore A 23187 across phospholipid vesicular membranes. A *H—NMR and theoretical study. Biophys. Chem. 8, 341—355,1978.
• TRELA Z., JANAS T., WITEK S., PRZESTALSKI S. Effects of quaternary ammonium salts on membrane potential and electric conductance in intemodal cells of Nitellopisi obtusa. Physiologia Plantarum 78, 57—61, 1990.
• HAUSER H„ DAWSON R.M.C. The displacement of calcium ions from phospholipid monolayers by pharmacologically active and other organic bases. Biochem. J. 109, 909—916,1968.