Activity of superoxide dismutase in Galleria mellonella larvae infected with entomopathogenic nematodes Steinernema affinis and S. feltiae

• Autorzy: Zoltowska K , Grochla P. , Lopienska-Biernat E.
• Języki publikacji: EN

Abstrakty

EN

Background. The influence of infection with two species of entomopathogenic nematodes of Steinernematidae family on the activity of superoxide dismutase (SOD) of the host was studied. Material and methods. Last instar larvae of Galleria mellonella were experimentally infected with Steinernema affinis and S. feltiae at 20 invasive juveniles per insect. At 6, 12, 18, 24 and 36 h after infection activity of SOD was determined in extracts from infected and control insects. Results. The activity of SOD decreased gradually in the controls during the experiment. The activity of enzyme was 2-4-times higher in insects from both infected groups than in the control. During the first 12 h of infection the activity of SOD in insects infected with S. feltiae was higher than in those infected with S. affinis, then the activity of enzyme in the insects of both infected groups stayed at a similar level. A significant decrease of SOD activity in infected was recorded in second day of the infection.

Słowa kluczowe

EN

parasitic disease; superoxide dismutase; Galleria mellonella; Steinernema feltiae; oxidative stress; animal disease; invasive disease; parasite; activity; parasitology; nematode; Steinernema affinis; larva; entomopathogenic nematode

• Wydawca: -
• Czasopismo: Wiadomości Parazytologiczne
• Rocznik: 2006
• Tom: 52
• Numer: 4
• Opis fizyczny: p.283-286,ref.

Twórcy

autor

Zoltowska K

• University of Warmia and Mazury, Oczapowskiego 1A St., 10-719 Olsztyn, Poland

autor

Grochla P.

autor

Lopienska-Biernat E.

Bibliografia

• [1] Bartosz G. 2003. Druga twarz tlenu. Wolne rodniki w przyrodzie. PWN, Warszawa.
• [2] Smith N.C., Ovigton K.S., Boray J. 1992. Fasciola hepatica: free radical generation by peritoneal leukocytes in challenged rodents. International Journal for Parasitology 22: 281-286.
• [3] Kazura J.W., Meshnik S.R. 1984. Scavenger enzymes and resistance to oxygen-mediated damage to Trichinella spiralis. Molecular and Biochemical Parasitology 10: 1-10.
• [4] Hadaś E., Gustowska L., Boczoń K., Janczewska D. 1999. Histochemical study of the nitric oxide synthase activity in experimental trichinellosis. Wiadomości Parazytologiczne 45: 63-68.
• [5] Becker K., Tilley L., Vennerstrom J.L., Roberts D., Rogerson S., Ginsburg H. 2004. Oxidative stress in malaria parasite-infected erythrocytes: host-parasite interactions. International Journal for Parasitology 34: 163-189.
• [6] Aucoin R.R., Philogene B.J.R., Arnason J.T. 1991. Antioxidant enzymes as biochemical defenses against phototoxin-induced oxidative stress in three species of herbivorous Lepidoptera. Archives of Insect Biochemistry and Physiology 16: 139-152.
• [7] Felton G.W., Summers C.B. 1995. Antioxidant systems in insects. Archives of Insect Biochemistry and Physiology 29: 187-197.
• [8] Weirich G.F., Collins A.M., Williams V.P. 2003. Antioxidant enzymes in the honey bee, Apis mellifera. Apidologie 33: 3-14.
• [9] Gaugler R., Kaya H.K. (Eds.). 1990. Entomopathogenic nematodes in biological control. CRC Press, Boca Raton, FL.
• [10] Żółtowska K., Łopieńska-Biernat E. 2006. Content of glycogen and trehalose and activity of α-amylase and trehalase in Galleria mellonella larvae infected with entomopathogenic nematodes Steinernema affinis and S. feltiae. Wiadomości Parazytologiczne 52: 103-107.
• [11] Bradford J. 1976. Arapid sensitive method for quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Analytical Biochemistry 72: 248-254.
• [12] Beauchamp C., Fridovich I. 1971. Superoxide dismutase improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry 44: 276-287.
• [13] Cox-Foster D.L., Fenimore J.A., Lazzaro B. 1998. Biochemistry of oxidative free radical production during innate immunity by insect hemocytes. Free Radical Biology and Medicine 25 (Suppl.1): S44.
• [14] Nappi A.J., Wass E. 1998. Hydrogen peroxide production in immune-reactive Drosophila melanogaster. Journal of Parasitology 84: 1150-1157.
• [15] Slepneva I.A., Komarov D.A., Glupov V.V., Srebrov V.V., Khramtsov V.V. 2003. Influence of fungal infection on DOPA-semiquinone and DOPA-quinone production in haemolymph of Galleria mellonella larvae. Biochemical and Biophysical Research Communications 300: 188-191.
• [16] Nappi A.J., Vass E., Frey F., Carton Y. 1995. Superoxide anion generation in Drosophila during the melanotoic encapsulation of parasites. European Journal of Cell Biology 68: 450-456.
• [17] Lipiński Z., Żółtowska K. 2005. Preliminary evidence associating oxidative stress in honey bee drone brood with Varroa destructor. Journal of Apicultural Research 44: 126-128.
• [18] Whitten M.M.A., Mello C.B., Gomes S.A.O., Nigam Y., Azambuja P., Garcia E.S. 2001. Role of superoxide and reactive nitrogen intermediates in Rhodnius prolixus (Reduviidae)/Trypanosoma rangeli interactions. Experimental Parasitology 98: 45-57.
• [19] Slepneva I.A., Glupov V.V., Sergeeva S.V., Khramtsov V.V. 1999. EPR detection of reactive oxygen species in hemolymph of Galleria mellonella and Dendrolimus superans sibiricus (Lepidoptera) larvae. Biochemical and Biophysical Research Communications 264: 212-215.