Concanavalin A-agarose removes mannan impurities from an extracellularly expressed Pichia pastoris recombinant protein

• Autorzy: Palczewska M , Batta G. , Groves P.
• Języki publikacji: EN

Abstrakty

EN

Pichia pastoris secretes few native proteins. However, the more than 1 g 1-1 of extracellularly expressed mannan interfered with the purification of our extracellularly expressed, non-glycosylated recombinant protein. Concanavalin A-agarose removed more than 95% of the unwanted mannan as monitored by phenol reaction. A 13C-based NMR assay confirmed this improvement. Concanavalin A-agarose can assist the purification of extracellular expressed, non-glycosylated proteins from yeasts.

Słowa kluczowe

EN

yeast; extracellular protein; native protein; Pichia pastoris; purification; concanavalin A; recombinant protein

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2003
• Tom: 08
• Numer: 3
• Opis fizyczny: p.783-792,fig.

Twórcy

autor

Palczewska M

• Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, Poland

autor

Batta G.

autor

Groves P.

Bibliografia

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