Activity of enzymes in zinc contaminated soil

• Autorzy: Wyszkowska J. , Zaborowska M. , Kucharski J.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study has been to determine the effect of soil contamination with zinc on the activity of soil enzymes. The study consisted of two laboratory experiments. Same, light loamy soil of pH 7.1, was used in both experiments. The variables in the first experiment were: a degree of soil contamination with zinc in mg Zn kg-1 d.m. of soil: 0, 5, 500, 1000, 1500 and 2000; dose of cellulose in g kg-1 d.m. of soil: 0, 15, and time of soil incubation (15 – 120 days). In the second experiment the following variables were tested: the degree of soil contamination with zinc in mg Zn kg-1 d.m. of soil: 0, 1000 and 2000; soil pH: 7.1, 6.4 and 5.5, and the time of soil incubation (15 – 120 days). The results of the experiments demonstrated that contamination of soil with zinc led to depressed activity of dehydrogenases, urease, acid phosphatase and alkaline phosphatase. Dehydrogenases and urease appeared to be more vulnerable to zinc contamination than phosphatases. The soil enzymes were adversely affected not only by zinc contamination but also by increasing soil acidity. According to their vulnerability to soil acidity the soil enzymes can be ordered as follows: dehydrogenases > urease > alkaline phosphatase > acid phosphatase. Cellulose added to soil (15 g kg-1) proved to be a good factor in the improvement of soil biochemical properties, although it did not limit the effects produced by zinc.

Słowa kluczowe

EN

acid phosphatase; alkaline phosphatase; enzyme; contamination; urease; dehydrogenase; zinc

• Wydawca: -
• Czasopismo: Electronic Journal of Polish Agricultural Universities. Series Environmental Development
• Rocznik: 2006
• Tom: 09
• Numer: 1
• Opis fizyczny: http://www.ejpau.media.pl/volume9/issue1/art-06.html

Twórcy

autor

Wyszkowska J.

• University of Warmia and Mazury in Olsztyn, Pl.Lodzki 3, 10-727 Olsztyn, Poland

autor

Zaborowska M.

autor

Kucharski J.

Bibliografia

• Alef K., Nannipieri P.; 1998. Urease activity. In: Methods in Applied Soil Microbiology and Biochemistry. Alef K., Nannipieri P. (eds), Academic press. Harcourt Brace & Company, Publishers, London; 316-320.
• Alef K., Nannipieri P., Trazar-Cepeda C.; 1998. Phosphatase activity. In: Methods in Applied Soil Microbiology and Biochemistry. Alef K., Nannipieri P. (eds), Academic press. Harcourt Brace & Company, Publishers, London; 335-344.
• Cordova A., Alvarez-Mon M.; 1995. Behavior of zinc in Physical Exercise: A special Reference to Immunity and Fatigue. Neuroscience and Biobehavioral Reviews.; 19(3): 439-445.
• Katayama A.,Wada A., Nishino T., Ishihama A.; 2002. Systematic search for zinc – binding proteins in Escherichia coli. Eur. J. Biochem.; 269: 2403-2413.
• Kieliszewska-Rokicka B.; 2001. Enzymy glebowe i ich znaczenie w badaniach aktywnosci mikrobiologicznej gleby. W: Drobnoustroje srodowiska glebowego – aspekty fizjologiczne, biochemiczne, genetyczne. [Soil enzymes and their importance in studies on the microbial activity of soil. In: Soil environment microorganisms – physiological, biochemical and genetic aspects]. (Ed. H. Dahm, A. Pokojska-Burdziej), UMK Toruń; 37-49; [in Polish].
• Kucharski J.; 1997. Relacje między aktywnosciš enzymów a żyznosciš gleby. W: Drobnoustroje w srodowisku, występowanie, aktywnosc i znaczenie. [Relationship between activity of enzymes and soil fertility. In: Microorganisms in environment, occurrence, activity and importance]. (Ed. W. Barabasz), AR Kraków; 327-342; [in Polish].
• Kucharski J., Wyszkowska J.; 2004. Inter-realtionship between number of microorganisms or spring barley yield and degree of soil contamination with copper. Plant Soil and Environment; 50(6): 243-249.
• McCall K.A., Huang Ch., Fierke C.A.; 2000 Function and mechanism of zinc metalloenzymes. J. Nutr.; 130: 1437-1446.
• Nannipieri P., Ascher J., Ceccherini M.T., Landi L., Pietramellara G., Renella G.; 2003. Microbial diversity and soil function. Eur. J. Soil Sci.; 54(4): 655-670.
• Nowak J., Szymczak J., Słobodzian T.; 2003. Próba okreslenia 50% progu toksycznosci dawek różnych metali ciężkich dla fosfataz glebowych. [The test of qualification 50% threshold of toxicity of doses different heavy metals for soil phosphatases]. Zesz. Probl. Post. Nauk Rol.; 492: 241–248; [in Polish].
• Smejkalova M., Mikanova O., Boruvka L.; 2003. Effects of heavy metal concentrations on biological activity of soil microorganisms. Plant Soil and Environment; 49(7): 321-326.
• StatSoft, Inc. 2003: STATISTICA (data analysis software system), version 6. www.statsoft.com.
• Öhlinger R.; 1996. Dehydrogenase activity with the substrate TTC. In: Methods in Soil Biology. Schinner F., Öhlinger R., Kandeler E., Margesin R. (eds), Springer Verlag Berlin Heidelberg; 241-243.
• Swištkiewicz S., Korelski J.; 2001. Cynk jako mikroelement niezbędny w nowoczesnej produkcji drobiarskiej. [Zinc as an element essential in modern poultry production]. Biul. Infor.; 39(2): 25-36; [in Polish].
• Trasar-Cepeda C., Leiros C., Gil – Sotres F., Seoane S.; 1998. Towards a biochemical quality Index for soils: An expression relating several biological and biochemical properties. Biol. Fertil. Soils; 26: 100-106.
• Welp G.; 1999. Inhibitory effect of the total water-soluble concentrations of nine different metals on the dehydrogenase activity of a loess soil. Biol. Fert. Soils; 30 (1-2): 132–139.
• Wong K.w., Toh B.A., Ting Y.P., Obbard J.P.; 2005. Biodegradation of phenanthrene by the indigenous microbial biomass in a zinc amended soil. Lett. Appl. Microbiol.; 40(1): 50-55.
• Wyszkowska J., Kucharski J.; 2003. Własciwosci biochemiczne i fizykochemiczne gleby zanieczyszczonej metalami ciężkimi. [Biochemical and physicochemical properties of the soil contaminated with heavy metals]. Zesz. Probl. Post. Nauk Rol.; 492: 435-442; [in Polish].
• Wyszkowska J., Kucharski J.; 2003. Effect of soil contamination with copper on its enzymatic activity and physicochemical properties. Electron. J. Pol. Agric. Univ.; 6(2).