Enhancing the biosynthesis of salidroside by biotransformation of p-tyrosol in callus culture of Rhodiola rosea L.

• Autorzy: Krajewska-Patan A , Furmanowa M. , Dreger M. , Gorska-Paukszta M. , Lowicka A. , Mscisz A. , Mielcarek S. , Baraniak M. , Buchwalld W. , Mrozikiewicz P.M.

Warianty tytułu

PL

Wzmozona biosynteza salidrozydu w wyniku biotransformacji p-tyrozolu w kulturach kalusowych rozenca gorskiego Rhodiola rosea L.

• Języki publikacji: EN

Abstrakty

EN

The aim of this research was to enhance the content of salidroside by exogenous addition of p-tyrosol in R. rosea tissue cultures. The callus tissue cultured on solid medium (MS with addition of NAA, BAP and adenine chloride) and compact callus agregate (CCA) were used in the experiments. The p-tyrosol was added to medium at a concentration of 5 mM/L (both into liquid and solid medium) and at concentration of 2.5 mM/L (only into liquid medium) in the day of the inoculation. The content of salidroside approximated: 23.15 mg/g (on solid medium) and 43.22 mg/g (CCA) after 7 days of 5 mM/L p-tyrosol application. The yield of salidroside was 3.1% (solid medium) and 4.3% (CCA). The addition of 2.5 mM tyrosol to CCA culture induced the increase of the content of salidroside to 34.73 mg/g and 3.5% yield of salidroside was obtained. The adverse effect was observed in biomass. Salidroside was not released into the medium.

PL

Celem badań było zwiększenie zawartości salidrozydu w kulturach kalusowych różeńca górskiego poprzez dodanie p-tyrozolu bezpośrednio do pożywki. Eksperyment obejmował zarówno kultury hodowane na pożywce stałej (wg Murashiga i Skooga z dodatkiem NAA, BA i chlorku adeniny), jak i na pożywce płynnej (CCA). Tyrozol dodawano w stężeniu 5 mM/ L i 2,5 mM/L (tylko w przypadku CCA) w dniu inokulacji. Po 7 dniach od dodania 5 mM tyrozolu zawartość salidrozydu wynosiła: 23,15 mg/g (kalus na stałej pożywce) i 43,22 mg/g (CCA). Zastosowanie 2,5 mM/L pozwoliło zwiększyć biosyntezę salidrozydu do zawartości 34.73 mg/g w CCA. Zaobserwowano również zmniejszony przyrost biomasy w stosunku do kontroli. Nie stwierdzono wydzielania salidrozydu do pożywki.

Słowa kluczowe

EN

biotransformation; salidroside; p-tyrosol; roseroot; biosynthesis; callus tissue; Crassulaceae; Arctic root zob.roseroot; Rhodiola rosea; Golden root zob.roseroot

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2007
• Tom: 53
• Numer: 1
• Opis fizyczny: p.55-64,fig.,ref.

Twórcy

autor

Krajewska-Patan A

• Research Institute of Medicinal Plant, Libelta 27, 61-707 Poznan, Poland

autor

Furmanowa M.

autor

Dreger M.

autor

Gorska-Paukszta M.

autor

Lowicka A.

autor

Mscisz A.

autor

Mielcarek S.

autor

Baraniak M.

autor

Buchwalld W.

autor

Mrozikiewicz P.M.

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