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2008 | 57 | 2 |

Tytuł artykułu

Influence of microencapsulation and spray drying on the viability of Lactobacillus and Bifidobacterium strains

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Improved production methods of starter cultures, which constitute the most important element of probiotic preparations, were investigated. The aim of the presented research was to analyse changes in the viability of Lactobacillus, acidophilus and Bifidobacterium bifidum after stabilization (spray drying, liophilization, fluidization drying) and storage in refrigerated conditions for 4 months. The highest numbers of live cells, up to the fourth month of storage in refrigerated conditions, of the order of 10⁷ cfu/g preparation were recorded for the B. bifidum DSM 20239 bacteria in which the N-Tack starch for spray drying was applied. Fluidization drying of encapsulated bacteria allowed obtaining a preparation of the comparable number of live bacterial cells up to the fourth month of storage with those encapsulated bacteria, which were subjected to freeze-drying but the former process was much shorter. The highest survivability of the encapsulated L. acidophilus DSM 20079 and B. bifidum DSM 20239 cells subjected to freeze-drying was obtained using skimmed milk as the cryoprotective substance. Stabilization of bacteria by microencapsulation can give a product easy to store and apply to produce dried food composition.

Wydawca

-

Rocznik

Tom

57

Numer

2

Opis fizyczny

p.135-140,fig.,ref.

Twórcy

autor
  • University of Life Sciences in Poznan, Wojska Polskiego 31, 60-624 Poznan, Poland
autor

Bibliografia

  • Burbianka M., A. Pliszka and E. Burzynska. 1983. Food Microbiology (in Polish). State Enterprise of Medical Publications, Warszawa.
  • Champagne CP. and N.J. Gardner. 2001. The effect of protective ingredients on the survival of immobilized cells of Streptococcus thermophilus to air and freeze-drying, Electron. J. Biotechnol. 4: 1-8. Champagne CP., F. Mondou, Y. Raymond and D. Roy. 1996. Effect of polymers and storage temperature on the stability of freeze-dried lactic acid bacteria. Food Res. Int. 29: 555-562.
  • Champagne CP., N. Morin, R. Couture, C. Gagnon, P. Jelen and C. Lacroix. 1992. The potential of immobilized cell technology to produce freeze-dried, phage-protected cultures Lactococcus lactis. Food Res. Int. 25: 419-427.
  • Chandramouli V., K. Kailasapathy, P. Peiris and M. Jones. 2004. An improved method of microencapsulation and its evaluation to protect Lactobacillus spp. in simulated gastric conditions. J. Microbiol. Meth. 56: 27-35.
  • Crittenden R., A. Laitila, P. Forssell, J. Mättö, M. Saarela, T. Mattila-Sandholm and P. Myllärinen. 2001. Adhesion of Bifidobacteria to granular starch and its implications in probiotic technologies. Appl. Environ. Microbiol. 67: 3469-3475.
  • Dembczynski R and T. Jankowski. 2002. Growth characteristics and acidifying activity of Lactobacillus rhamnosus in alginate/starch liquid-core capsules. Enzyme Microb. Technol. 31: 111-115.
  • Dembczynski R. and T. Jankowski. 2000. Characterization of small molecules diffusion in hydrogel-membrane liquid-core capsules. Biochem. Eng. J. 6: 41-44.
  • Desmond C, C. Stanton, G.F. Fitzgerald, K. Collins and R.P. Ross. 2001. Environmental adaptation of probiotic lacto-bacilli towards improvement of performance during spray drying, Int. Dairy J. 11 : 801-808.
  • Gardiner G.E., P. Bouchier, E. O'Sullivan, J. Kelly, J.K. Collins, G. Fitzgerald, R.P. Ross and C. Stanton. 2002. A spray-dried culture for probiotic Cheddar cheese manufacture. Int. Dairy J. 12: 749-756.
  • Lee K.Y. and T.R. Heo. 1999. Survival of Bifidobacterium longum immobilized in calcium alginate beads in simulated gastric juices and bile salt solution. Appl. Environ. Microbiol. 66: 869-873.
  • Schlegel H.G. 2000. General Microbiology (in Polish), (ed) PWN. Warszawa.
  • Witrowa-Rajchert D. and K. Samborska. 2002. Drying methods of microorganisms and products of microbiological synthesis (in Polish). Food Sci. Technol. Quality: 5-15.
  • Yoo I.K., G.H. Seong, H.N. Chang and J.K. Park. 1996. Encapsulation of Lactobacillus casei cells in liquid-core alginate capsules for lactic acid production. Enzyme Microbiol. Technol. 19: 428-433.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-b58e5ac4-2dfb-4078-bd5d-e376235b929c
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