Ocena przydatnosci wybranych chromosomalnych markerow do identyfikacji Bacillus anthracis. II. Ocena markerow SSH oraz RPOB

• Autorzy: Zasada A. A. , Jagielski M.

Warianty tytułu

EN

Valuation for usefulness of selected chromosomal markers for Bacillus anthracis identification. II. Valuation for markers SSH and RPOB

• Języki publikacji: PL

Abstrakty

PL

Zbadano 53 szczepy Gram-ujemnych pałeczek z rodziny Enterobacteriaceae oraz z grupy pałeczek niefermentujących glukozy. Oznaczono fenotypy oporności na antybiotyki aminoglikozydowe metodą dyfuzyjno-krążkową, na podstawie których określono prawdopodobne klasy enzymów (AAC, ANT, APH) modyfikujących aminoglikozydy. Wartości MIC dla czterech podstawowych aminoglikozydów oznaczono za pomocą E-testów. Ponadto określono wrażliwość badanych szczepów na inne antybiotyki.

EN

The aim of the study was to evaluate the aminoglycoside resistance of Gram-negative bacilli isolated from patients. To the examination 35 strains of Enterobacteriaceae and 18 of non-fermentative bacteria were included. Resistance to aminoglycosides (gentamicin (G), netilmicin (Nt), tobramycin (T), amikacin (A), kanamycin (K), neomycin (N)) was established by disk diffusion method. Interpretation of enzymatic mechanisms was performed by Livermore. The most common enzymes AAC(6')I were found in Enterobacteriaceae group (mostly in E. cloaceae and P. mirabilis) and ААС(З') and in non-fermentative bacteria: AAC(6')I in P. aeruginosa and APH(3')VI and AAC(3')I in A. baumanii. The most frequent phenotype was resistance to six antibiotics (G, Nt, T, A, K, N) Resistance rates were high for gentamicin (>70 %) in both groups and amikacin (88,89 %) in non-fermentatives.

Słowa kluczowe

PL

markery chromosomowe; ocena przydatnosci; markery SSH; marker RPOB; Bacillus anthracis; marker SSH241; marker SSH196; marker SSH163; marker SSH133; gen rpoB; biologia molekularna

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2006
• Tom: 58
• Numer: 4
• Opis fizyczny: s.355-362,rys.,tab.,bibliogr.

Twórcy

autor

Zasada A. A.

• Panstwowy Zaklad Higieny w Warszawie, ul.Chocimska 24, 00-791 Warszawa

autor

Jagielski M.

Bibliografia

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