Flow cytometric analysis of nuclear replication stages during germination of sugar-beet seeds different in vigour

• Autorzy: Sliwinska E. , Pedersen H.C.
• Języki publikacji: EN

Abstrakty

EN

The nuclear replication stages in dry and germinating seeds of two sugar-beet cultivars were determined by flow cytometry. For each cultivar two seed lots of similar, high germination capacity, but different in seedling length vigour index (SLVI; applied as quality marker) were studied. Analyses were performed every 12 hours during 72 hours of germination and early seedling growth at 20oC and 15oC. Before start of germination, in all seed lots, about 90% of cells were arrested in the G1 phase of the cell cycle. The proportion of G1 cells decreased with time of germination, faster in higher quality seed lots than in those of lower quality. The G2/G1 ratio in dry seeds was slightly higher for the seeds of higher SLVI, and it increased during germination in all seed lots. However, the difference between higher and lower quality seeds of the same cultivar did not become more distinct during germination.

Słowa kluczowe

EN

cell cycle; Beta vulgaris; DNA replication; flow cytometry; vigor; germination; seed; sugar-beet

• Wydawca: -
• Czasopismo: Electronic Journal of Polish Agricultural Universities. Series Agronomy
• Rocznik: 1999
• Tom: 02
• Numer: 1
• Opis fizyczny: http://www.ejpau.media.pl/series/volume2/issue1/agronomy/art-01.html

Twórcy

autor

Sliwinska E.

• University of Technology and Agriculture, Kaliskiego 7, 85-796 Bydgoszcz, Poland

autor

Pedersen H.C.

Bibliografia

• Bewley J.D., Black M., 1994. Seeds: physiology of development and germination. Plenum Press, New York and London.
• Bino R.J., De Vries J.N., Kraak H.L., Van Pijlen J.G., 1992. Flow cytometric determination of nuclear replication stages in tomato seeds during priming and germination. Ann. Bot. 69, 231-236.
• Bino R.J., Lanteri S., Verhoeven H.A., Kraak H.L., 1993. Flow cytometric determination of nuclear replication stages in seed tissues. Ann. Bot. 72, 181-187.
• Butterfass T. and Schlayer G., 1965. Widersprüchliche Ergebnisse bei der Bestimmung des Endopolyploidiegrads von Zuckerrüben durch Auslösung von Mitosen. Chromosoma 17, 303-308.
• Deltour R., 1985. Nuclear activation during early germination of the higher plant embryo. J. Cell Sci. 75, 43-83.
• Galbraith D.W., Harkins K.R., Maddox J.M., Ayres N.M., Sharma D.P., Firoozabady E., 1983. Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220, 1049-1051.
• Georgieva E.I., Lopez-Rodas G., Hittmair A., Feichtinger H., Brosch G., Loidl P., 1994. Maize embryo germination. I. Cell cycle analysis. Planta 192, 118-124.
• Grimwade J.A., Grierson D., Whittington W.J., 1987. The effect of differences in time to maturity on the quality of seed produced by different varieties of sugar beet. Seed Sci. Technol. 15, 135-145.
• ISTA (International Seed Testing Association), 1985. International Rules for Seed Testing. Seed Sci. Technol. 13, 322-447.
• Jassem M. and Sadowski H., 1990. Seed improvement as a factor increasing sugar beet growing (in Polish). Biul. IHAR 173-174, 155-165.
• Jassem M., Sliwinska E., Zornow A., 1993. The influence of substrate moisture on germination capacity of sugar-beet seeds. Seed Sci. Technol. 21, 203-211.
• Lanteri S., Kraak H.L., De Vos C.H.R., Bino R.J., 1993. Effects of osmotic preconditioning on nuclear replication activity in seeds of pepper (Capsicum annuum). Physiol. Plant. 89, 433-440.
• Lanteri S., Saracco F., Kraak H.L., Bino R.J., 1994. The effects of priming on nuclear replication activity and germination of pepper (Capsicum annuum) and tomato (Lycopersicon esculentum) seeds. Seed Sci. Res. 4: 81-87.
• Liu Y., Bergervoet J.H.W., De Vos C.H.R., Hilhorst W.M., Kraak H.L., Karssen C.M., Bino R.J., 1994. Nuclear replication activities during imbibition of abscisic acid- and gibberellin-deficient tomato (Lycopersicon esculentum) seeds. Planta 194, 368-373.
• Sliwinska E., 1996. Flow cytometric analysis of the cell cycle of sugar-beet seed during germination. Gen. Pol. 37A, 254-257.
• Sliwinska E., 1997. Flow cytometric analysis of sugar-beet seeds different in vigour. In: Basic and Applied Aspects of Seed Biology, Ellis R.H., Black M., Hong T.D. (eds). Kluwer Academic Publishers, Dodrecht, 577-584.
• Sliwinska E., 1998. Cell cycle activity during development of sugar-beet seed. In: Plant Cytogenetics, Maluszynska J. (ed.). Prace Naukowe Uniwersytetu Slaskiego 1696, 51-59.
• Sliwinska E., Jing Hai-Chun Job C., Job D., Bergervoet J.H.W., Bino R.J., Groot S.P.C., 1999. Effect of harvest time and soaking treatment on cell cycle activity in sugar-beet seeds. Seed Sci. Res. 9 (in press).
• Sliwinska E. and Steen P., 1995. Flow cytometric estimation of ploidy in anisoploid sugar-beet populations. J. Appl. Genet. 36(2), 111-118.