Przydatnosc ELISA do diagnozowania wlosnicy u swin i dzikow

• Autorzy: Bien J

Warianty tytułu

EN

The usefulness of ELISA for diagnosis of trichinellosis in pigs and wild boars

• Języki publikacji: PL

Abstrakty

EN

ELISA can be used to measure produced antibodies or Trichinella spp. antigens in the samples. They are detected with antibodies linked to an enzyme that reacts with a substrate and generate a colour reaction. The optical density (OD) of the reaction is measured spectrophotometrically. ELISA assays can be done in several different procedures called „direct”, "indirect", "sandwich", and "competition" ELISA. Since the 1970s, the studies have been done on improving or replacing direct methods of Trichinella diagnosis with serological methods based on the ELISA. When somatic antigens of L1 T. spiralis were used, the specificity of the ELISA was poor due to a high probability of cross-reactions with other pathogens. During the 1980s the specificity of the ELISA was improved by excretory-secretory (E/S) antigens obtained during Trichinella muscle larvae incubation in vitro. Recently a synthetic glycan antigen has been developed and the increasing of ELISA specificity and sensitivity was noticed. The sensitivity of the ELISA using an E/S antigen ranging from 93.1 to 99.2% but the specificity from 90.6 to 99.4%. The ELISA method is relatively simple to apply, reliable, readily standardized and provides an acceptable balance of sensitivity and specificity. But all modified procedures should be validated. In Poland, the studies on the usefulness of ELISA for antibodies detection against T. spiralis in pigs and wild animals are limited. Own ELISA procedure was prepared in Pathophysiology Lab. in W. Stefański Institute of Parasitology of PAS. ELISA was used to examine IgG level against L1 T. spiralis in pigs and wild boars serum samples. Of 1474 pig samples, only 12 were positive. Of 1880 wild boar samples only 14 were positive. The results of this study are comparable with performance obtained using commercial sets. The results showed the usefulness of ELISA for T. spiralis diagnosis in pigs and wild boars and confirmed the possibility of use the ELISA test for application in the slaughterhouse.

Słowa kluczowe

PL

wlosnica; choroby pasozytnicze; diagnostyka weterynaryjna; Polska; walidacja; pasozyty; Warszawa konferencja; trzoda chlewna; dziki; wykorzystanie; parazytologia; konferencje; Trichinella; choroby zwierzat; test ELISA; wykrywanie

• Wydawca: -
• Czasopismo: Wiadomości Parazytologiczne
• Rocznik: 2006
• Tom: 52
• Numer: 3
• Opis fizyczny: s.205-212,rys.,bibliogr.

Twórcy

autor

Bien J

• Instytut Parazytologii PAN, ul.Twarda 51/55, 00-818 Warszawa

Bibliografia

• [1] Ruitenberg E.J., Steerenberg P.A., Brosi B.J.M., Buys J. 1974. Serodiagnosis of Trichinella spiralis infections in pigs by enzyme-linked immunosorbent assays. Bulletin of the World Health Organization 51: 108-109.
• [2] Ruitenber E.J., Steerenberg P.A., Brosi B.J.M., Buys J. 1975. ELISA (Enzyme-linked immunosorbent assay) as preventive and repressive control method for the detection of Trichinella spiralis infections in slaughter pigs. Wiadomości Parazytologiczne 31: 747-751.
• [3] Gamble H.R., Anderson W.R., Graham C.E., Murrell K.D. 1983. Diagnosis of swine trichinellosis by enzyme-linked immunosorbent assay (ELISA) using an excretory-secretory antigen. Veterinary Parasitology 13: 349-361.
• [4] Gamble H.R., Wiśniewski N., Wassom D. 1997. Diagnosis of trichinellosis in swine by enzyme immunoassay using a synthetic glycan antigen. American Journal of Veterinary Research 58: 417-421.
• [5] Nasinyama G.W., Gordon J.C., Bech-Nielsen S., Barriga O.O. 1991. IgG response in guinea pigs to Trichinella spiralis infection. Veterinary Parasitology 39: 301-311.
• [6] van der Leek M.L., Dame J.B., Adams C.L., Gillis K., Littell R.C. 1992. Evaluation of an enzyme-linked immunosorbent assay for diagnosis of trichinellosis in swine. American Journal of Veterinary Research 53: 877-882.
• [7] Nöckler K., Pozio E., Voigt W.P., Heidrich J. 2000. Detection of Trichinella infection in food animals. Veterinary Parasitology 93: 335-350.
• [8] Burgess G.W. 1988. ELISA technology in diagnosis and research. James Cook University of North Queensland, Townsville, Australia.
• [9] Dzbeński T.H., Bitkowska E., Płonka W. 1994. Detection of circulation parasitic antigen in acute infection with Trichinella spiralis: diagnostic significance of findings. Zentralblatt für Bakteriologie 281: 519-525.
• [10] Manual of standards for diagnostic tests and vaccines 2000. Office International des Epizooties, Paris, France: 322-327.
• [11] Gamble H.R., Pozio E., Bruschi F., Nöckler K., Kapel C.M.O., Gajadhar A.A. 2004. International Commission on Trichinellosis: recommendations on the use of serological tests for the detection of Trichinella infection in animals and man. Parasite 11: 3-13.
• [12] Kapel C.M.O., Gamble H.R. 2000. Infectivity, persistence and antibody response to domestic and sylvatic Trichinella spp. experimentally infected pigs. International Journal for Parasitology 30: 215-221.
• [13] Grundy M. 1982. Preliminary observations using a multilayer ELISA method for detection of Entameba histolitica trophozoite antigens in stool samples. Transaction of the Royal Society of Tropical Medicine and Hygiene 76: 396-400.
• [14] Ghandi B., Irshad M., Acharya S., Samantray J., Tandon B. 1989. Use of affinity purified heterologus antibodies in an ELISA for detection of Entameba histolitica in faecal specimens. National Medical Journal of India 2: 223-227.
• [15] Kabil S., Hassan M., Atta M., El-Ghysha O. 1990. ELISA in detection of Entameba histolitica antigens in stool. Journal of the Egyptian Society of Parasitology 20: 673-676.
• [16] Wonsit R., Thammapalero N., Tharavanus S., Radomyos P., Bunnag D. 1992. Enzyme-linked immunosorbent assay based on monoclonal and polyclonal antibodies for detection of Entameba histolotica antigens in faecal specimens. Transaction of the Royal Society of Tropical Medicine and Hygiene 86: 166-169.
• [17] Ungar B., Yolken R., Nash T., Quinn T. 1984. Enzyme-linked immunosorbent assay for the detection of Giardia lamblia in fecal specimens. The Journal of Infectious Diseases 49: 90-97.
• [18] Green E., Miles M., Warhurst D. 1985. Immunodiagnostic detection of Giardia antigen in faeces by a rapid visual enzyme-linked immunosorbent assay. Lancet 2: 691-693.
• [19] Espino A.M., Marcet R., Finalay C.M. 1997. Fasciola hepatica: detection of antigenemia and coproantigens in experimentally infected rats. Experimental Parasitology 85: 117-120.
• [20] Dumenigo B.E., Mezo M. 1999. Monoclonal antibody sandwich immunoassay detection of coproantigen to evaluate the efficacy of treatment in natural ovine fasciolosis. Research in Veterinary Science 66: 165-167.
• [21] Allan J., Craig P. 1989. Coproantigen in gut tapeworm infections: Hymenolepis diminuta in rats. Parasitology Research 76: 68-73.
• [22] Allan J., Avila J., Garcia Noval J., Flisser A., Craig P. 1990. Immunodiagnosis of taeniasis by coproantgen detection. Parasitology 101: 473-477.
• [23] Deplazes P., Gottstein B., Eckert J., Jenkins D., Ewald D., Jimenez-Palacios S. 1992. Detection of Echinococcus coproantigens by enzyme-linked immunosorbent assay in dogs, dingoes and foxes. Parasitology Research 78: 303-308.
• [24] Boulos L.M., Ibrahim I.R., Negm A.Y., Aly S.M. 2001. Detection of coproantigen in early trichinellosis. Parasite 8: 136-139.
• [25] Engvall E., Ljungström J. 1975. Detection of human antibodies to Trichinella spiralis by enzyme-linked immunosorbent assay ELISA. Acta Pathologica et Microbiologica Scandinavica 83: 231-237.
• [26] van Knapen F., Franchimot J.H., Verdonk A.R., Stumpf J., Undeutsch K. 1982. Detection of specific immunoglobulins (IgG, IgM, IgA, IgE) and total IgE levels in human trichinosis by means of the enzyme-linked immunosorbent assay (ELISA). American Journal of Tropical Medicine Hygiene 31: 973-976.
• [27] Tomasik J., Grzybowski J., Golińska Z., Prokopowicz D. 1992. Activity of specific IgG, IgM and IgE antibodies in human trichinellosis. Wiadomości Parazytologiczne 38: 127-133.
• [28] Machnicka B., Prokopowicz D., Madaliński K., Dziemian E., Prokopowicz K., 1994. Antibodies, circulating antigens and circulating immune complexes in human trichinellosis. In: Trichinellosis. (Red. Campbell, W.C., Pozio, E., Bruschi, F.). Istituto Superiore di Sanita Press, Rome, Italy, 341-346.
• [29] Golińska Z. 1984. Przeciwciała powstające pod wpływem stymulacji antygenami pasożytów. Wiadomości Parazytologiczne 30: 73-83.
• [30] Gamble H.R. 1996. Detection of trichinellosis in pigs by artificial digestion and enzyme immunoassay. Journal of Food Protection 59: 295-8.
• [31] Geniteau M., Verroust P.J., Smith M.D., Morel-Maroger L., Saimot G., Coulaud J.P. 1977. Circulating immune complex in six patients with trichinosis. Clinical and Experimental Immunology 30: 141-144.
• [32] Dziemian E., Machnicka B. 2000. Influence of Trichinella spiralis infective dose on the level of antibodies, circulating antigens and circulating immune complexes in rats. Helminthologia 37: 59-66.
• [33] Kocięcka W. 1996. Włosień kręty i włośnica. Volumed, Wrocław.
• [34] Ruitenberg E.J., Steerenberg P.A., Brosi B.J.M., Buys J. 1976. Reliability of the enzyme-linked immunosorbent assay (ELISA) for diagnosis of Trichinella spiralis infections in conventionally raised pigs. Tijdschrift voor Diergeneeskunde 101: 57-68.
• [35] Cuperlovic K., Djordjevic M., Pavlovic S. 2004. Reemergence of trichinellosis in southeast Europe due to political and economic changes. XI International Conference on Trichinellosis. 8-12 August, 2004, San Diego, 23.
• [36] Forbes L.B. 2004. Food safety risk associated with trichinellosis in marine mammals. XI International Conference on Trichinellosis. 8-12 August, 2004, San Diego, 24.
• [37] Lind P., Eriksen L., Henrisen S.A., Homan W.L., van Knapen F., Nansen P., Stahl P. 1991. Diagnostic tests for Trichinella spiralis infection in pigs. A comparative study of ELISA for specific antibody and histamine release from blood cells in experimental infections. Veterinary Parasitology 39: 241-252.
• [38] Chapa-Ruiz M.R., Salinas-Tobon M.R., Aguilar-Alvarez D.J., Martinez-Moranon R. 1992. Recognition of Trichinella spiralis muscle larvae antigens by sera from human infected with this parasite and its potential use in diagnosis. Revista Latinoamericana de Microbiologia 34: 95-99.
• [39] Ruitenberg E.J., van Knapen F. 1977. Enzyme-linked immunosorbent assay (ELISA) as a diagnostic method for Trichinella spiralis infections in pigs. Veterinary Parasitology 3: 317-326.
• [40] Zarlenga D.S., Gamble H.R. 1990. Molecular cloning and expression of an immunodominant 53-kDa excretory-secretory antigen from Trichinella spiralis muscle larvae. Molecular and Biochemical Parasitology 42: 165-174.
• [41] Bruschi F., Moretti A., Wassom D., Piergili-Fioretti D. 2001. The use of a synthetic antigen for the serological diagnosis of human trichinellosis. Parasite 8: 141-143.
• [42] Moller L.N., Petersen E., Kapel C.M.O. 2005. Comparison of two antigens for demonstration of Trichinella spp. antibodies in blood and muscle fluid of foxes, pigs and wild boars. Veterinary Parasitology 132: 81-84.
• [43] Greiner M., Kumar S., Kyeswa C. 1997. Evaluation and comparison of antibody ELISA for serodiagnosis of bovine trypanomosis. Veterinary Parasitology 73: 197-205.
• [44] Greiner M., Gardner I.A. 2000. Epidemiologic issues in the validation of veterinary diagnostic tests. Preventive Veterinary Medicine 45: 3-22.
• [45] Whiting P., Rutjes A.W., Reitsma J.B., Glas A.S., Bossuyt P.M., Kleijnen J. 2004. Sources of variation and bias in studies of diagnostic accuracy: a systematic review. Annals of Internal Medicine 140: 189-202.
• [46] van Knapen F., Franchimont J.H., Ruitenberg E.J. 1980. The reliability of the enzyme linked immunosorbent assay (ELISA) for the detection of swine trichinellosis. Trichinellosis (Proceeding of the International Conference on Trichinellosis) 5: 399-404.
• [47] Ramisz A., Szymborski J., Balicka-Ramisz A. 1996. Ocena stosowanych w Polsce metod rozpoznawania włośnicy. Życie Weterynaryjne 10: 333-337.
• [48] Główny Inspektorat Weterynarii 2003 rok.
• [49] Główny Inspektorat Weterynarii 2004 rok.