In vitro micropropagation of 'Khalas' date palm [Phoenix dactylifera L.], an important fruit plant

• Autorzy: Aslam J , Khan S.A.

Warianty tytułu

PL

Rozmnazanie in vitro palmy daktylowej [Phoenix dactylifera L.] 'Khalas', waznej rosliny ze wzgledu na owoce

• Języki publikacji: EN

Abstrakty

EN

A protocol has been developed for an efficient tissue culture cycle (callus induction, callus growth, plant regeneration and root induction) for date palm 'Khalas' using various plant growth regulators (PGR) in both liquid and solidified MS medium. The shoot explants showed high callus induction potential on MS medium supplemented with 45.24 ^M 2,4-dichlorophenoxyacetic acid (2,4-D) followed by 54.21 ^M 2,4,5-trichlorophenoxyacetic acid (2,4,5-T). The highest frequency of shoot regeneration (85%) and number of shoots per an explant (5.6) were obtained on solid MS medium supplemented with 7.84 ^M N6-benzylaminopurine (BAP). Rooting (87.34%) was high on solid MS medium supplemented with 24.6 ^M indole-3-butyric acid (IBA). However, the root length was higher in MS liquid medium. This protocol will be useful for rapid, large-scale propagation of 'Khalas' date palm.

PL

Opracowano skuteczną metodę rozmnażania w kulturach tkankowych palmy daktylowej (indukcja kalusa, wzrost kalusa, regeneracja pędu i korzeni) stosując regulatory wzrostu (PGR) zarówno w po żywce MS płynnej, jak i zestalonej. Eksplantaty pędów wykazywały wysoki potencjał indukcji kalusa na pożywce MS uzupełnionej kwasem 2,4-dwuchlorofenoksyoctowym (2,4-D) w stężeniu 45,24 ^M i kwasem 2,4,5-trójchlorofenoksyoctowym (2,4,5-T) w stężeniu 54,24 ^M. Najwyższą regenerację pędów (85%) i liczbę pędów na eksplantacie (5,6) otrzymano na zestalonej po żywce MS uzupełnionej N6-benzyloaminopuryną (BAP) w stężeniu 7,84 ^M. Ukorzenianie (87,34%) było wysokie na zestalonej pożywce MS zawierającej kwas indolilomasłowy (IBA) w stężeniu 24,6 ^M. Korzenie byty najdłuższe na płynnej pożywce MS. Metoda ta jest użyteczna dla szybkiego rozmnażania palmy daktylowej 'Khalas' w dużych ilościach.

Słowa kluczowe

EN

date palm; Phoenix dactylifera; rooting; shoot multiplication; in vitro; Khalas cultivar; fruit plant; plant regeneration; tissue culture; micropropagation; plant growth regulator

• Wydawca: -
• Czasopismo: Journal of Fruit and Ornamental Plant Research
• Rocznik: 2009
• Tom: 17
• Numer: 1
• Opis fizyczny: p.15-27,fig.,ref.

Twórcy

autor

Aslam J

• Dubai Pharmacy College, Al Muhaisanah 1, Al Mizhar, P.O.Box 19099, United Arab Emirates

autor

Khan S.A.

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