Antioxidant capacity and its components of cruciferous sprouts

• Autorzy: Zielinski H. , Piskula M.K. , Michalska A. , Kozlowska H.

Warianty tytułu

PL

Pojemnosc przeciwutleniajaca kielkow nasion roslin krzyzowych oraz jej skladniki

• Języki publikacji: EN

Abstrakty

EN

This paper describes the antioxidant capacity of cruciferous sprouts and its components in the course of germination under light conditions. The content of soluble proteins (SP), reduced glutathione (GSH), L-ascorbic acid (AH2), tocopherols (a-T, b-T, g-T, d-T) and total phenolic compounds (TPC), and finally Trolox equivalent antioxidant capacity (TEAC) of the seeds and sprouts were determined in this respect. The results obtained were used for calculating the contribution of these compounds to total antioxidant capacity (TAC) of seeds and sprouts. The TAC of the samples was calculated as the sum of TEAC obtained by ABTS test (formed by TPC and AH2 presence) plus sum of the antioxidant capacities provided by tocopherols, soluble proteins and GSH. The percentage contribution of TPC was corrected by the content of AH2 due to the overestimated TPC values determined by Folin-Ciocalteu (FC) reagent, and a simple correction method is shown. Both corrected TPC and AH2 contribution was above 99%, 83% and 59% in radish, small radish and rapeseeds and about 97%, 73% and 71% in 5-day sprouts, respectively. The contribution of SP, GSH and T (a-T, b-T, g-T, d-T) in forming the antioxidant screen of the seeds and sprouts was of a minor importance since it did not exceed 6% in the seeds and approximately 2% in the sprouts collected after the fourth day of germination. This study indicates TPC and AH2 to be the most important antioxidants in cruciferous sprouts.

PL

W pracy badano pojemność przeciwutleniającą (TEAC) kiełkowanych do 7 dni w świetle nasion rzodkwi, rzodkiewki i rzepaku, oraz przeanalizowano w uzyskanych kiełkach zawartość białka rozpuszczalnego (SP), zredukowanego glutationu (GSH), kwasu L-askorbinowego (AH2), tokoferoli (T) oraz związków fenolowych ogółem (TPC). Uzyskane dane wykorzystano do obliczenia udziału tych związków w kształtowaniu całkowitej pojemności przeciwutleniającej (TAC) kiełków. Przedstawiono prostą metodę korekcji udziału związków fenolowych w TAC uwzględniającą zawartość AH2 w kiełkach. Badania pokazały, że skorygowany udział związków fenolowych oraz kwasu askorbinowego w kształtowaniu pojemności przeciwutleniającej gotowych do spożycia kiełków rzodkwi, rzodkiewki i rzepaku stanowił ponad 97%, 73% i 71% (tab. 3). Udział białek rozpuszczalnych, zredukowanego glutationu oraz tokoferoli w kształtowaniu TEAC nasion nie przekraczał 6%, natomiast w 4 dniowych kiełkach wynosił około 2% (tab. 3). Przeprowadzone badania wskazują, że zarówno związki fenolowe jak i kwas L-askorbinowy stanowią najważniejsze przeciwutleniacze obecne w kiełkach rzodkwi, rzodkiewki i rzepaku.

Słowa kluczowe

EN

functional food; new functional food; cruciferous plant; germination; sprout; antioxidant capacity; component; seed; light condition; cruciferous sprout

• Wydawca: -
• Czasopismo: Polish Journal of Food and Nutrition Sciences
• Rocznik: 2007
• Tom: 57
• Numer: 3
• Opis fizyczny: p.315-321,fig.,ref.

Twórcy

autor

Zielinski H.

• Polish Academy of Sciences, Tuwima 10, 10-718 Olsztyn, Poland

autor

Piskula M.K.

autor

Michalska A.

autor

Kozlowska H.

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