Isolation of outer membrane proteins [OMP] from Salmonella cells using zwitterionic detergent and their separation by two-dimensional electrophoresis [2-DE]

• Autorzy: Futoma-Koloch B , Bugla-Ploskonska G. , Doroszkiewicz W.
• Języki publikacji: EN

Abstrakty

EN

We report that using the zwitterionic detergent Zwittergent Z 3-14® to isolate outer membrane proteins (OMPs) from Salmonella O48 is suitable for their separation by two-dimensional electrophoresis (2-DE) in a capillary tube system. Sample preparation is a very crucial step for any bacterial proteomic study. Some modifications were introduced to the 2-DE protocol suggested by O'Farrell and BioRad, which significantly impaired the resolution of proteins. 2-DE analysis of OMPs may be helpful in the interpretation of the variable susceptibility of Salmonella O48 rods to the bactericidal activity of serum.

Słowa kluczowe

EN

cell; isolation; separation; Zwittergent Z 3-14 detergent; two-dimensional electrophoresis; outer membrane protein; Salmonella

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2009
• Tom: 58
• Numer: 4
• Opis fizyczny: p.363-366,fig.,ref.

Twórcy

autor

Futoma-Koloch B

• University of Wroclaw, Przybyszewskiego 63/77, 51-148 Wroclaw, Poland

autor

Bugla-Ploskonska G.

autor

Doroszkiewicz W.

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