Characterization of a nucleopolyhedrovirus isolated from the laboratory rearing of the beet armyworm Spodoptera exigua [Hbn.] in Poland

• Autorzy: Jakubowska A , Vlak J.M. , Ziemnicka J.

Warianty tytułu

PL

Charakterystyka wirusa wyizolowanego z laboratoryjnej hodowli swiatlolowki naziemnicy Spodoptera exigua [Hbn.] w Polsce

• Języki publikacji: EN

Abstrakty

EN

A nucleopolyhedrovirus isolated from the beet armyworm Spodoptera exigua (Polish laboratory culture), SeMNPV (P), morphologically similar to the viral bioinsecticide virus Spod-XR , was characterized molecularly and biologically. Phylogenetic analysis based on three conserved baculovirus genes, polh, lef-8 and pif-2, showed the highest homology of SeMNPV (P) to Mamestra brassicae (Mb) MNPV and M. configurata (Maco) MNPV, and much less to SeMNPV (Spod-XR). These findings were confirmed by genomic DNA restriction profile analyses. Bioassays revealed that SeMNPV isolated from the commercial bioinsecticide Spod-XR was themost infectious for S. exigua, while the infectivity of SeMNPV (P) and MbMNPV was significantly lower. These data suggest that SeMNPV (P) is a variant of MbMNPV.

PL

Scharakteryzowano pod względem molekularnym i biologicznym wirusa poliedrozy jądrowej (NPV) SeMNPV(P) pochodzącego z gąsienic światłołówki naziemnicy Spodoptera exigua (polska hodowla laboratoryjna) podobnego morfologicznie do aktywnego wirusowego składnika biopreparatu Spod-XR, SeMNPV(Spod-XR ). Analiza filogenetyczna polskiego izolatu wirusa oparta na trzech genach bakulowirusa polh, lef-8 i pif-2 wykazała niewielką homologię z wirusem SeMNPV(Spod-XR ) natomiast wysoką z wirusami Mamestra brassicae MbMNPV oraz Mamestra configurata MacoMNPV. Homologia ta została potwierdzona przez restrykcyjną analizę genomowego DNA. Wyniki testów biologicznych wykazały, że najbardziej aktywnym dla gąsienic S. exigua był wirus SeMNPV pochodzący z biopreparatu Spod-XR, natomiast aktywność wirusów SeMNPV-P i MbMNPV była porównywalnie niższa.

Słowa kluczowe

EN

plant protection; nucleopolyhedrovirus; isolation; beet armyworm; Spodoptera exigua; Poland; genomics; biological activity; Mamestra brassicae; small mottled willow moth zob.beet armyworm; laboratory culture; pest insect; bioinsecticide

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2005
• Tom: 45
• Numer: 4
• Opis fizyczny: p.279-286,fig.,ref.

Twórcy

autor

Jakubowska A

• Institute of Plant Protection, Miczurina 20, 60-318 Poznan, Poland

autor

Vlak J.M.

autor

Ziemnicka J.

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