Up-regulation of human PNPase mRNA by beta-interferon has no effect on protein level in melanoma cell lines

• Autorzy: Gewartowski K. , Tomecki R. , Muchowski L. , Dmochowska A. , Dzwonek A. , Malecki M. , Skurzak H. , Ostrowski J. , Stepien P.P.
• Języki publikacji: EN

Abstrakty

EN

Human mitochondrial polynucleotide phosphorylase (hPNPase) is an exoribonuclease localized in mitochondria. The exact physiological function of this enzyme is unknown. Recent studies have revealed the existence of a relationship between induction of hPNPase mRNA and both cellular senescence and growth arrest of melanoma cells following β-interferon treatment. The aim of this study was to verify whether the augmented hPNPase mRNA level results in increase of the protein level. In several cell lines established from five metastatic melanoma patients we did not find any such correlation. However, an elevated level of hPNPase protein was observed in interferon-induced HeLa and Jurkat cells. This increase was correlated with a slight shortening of poly(A) tails of mitochondrial ND3 transcript.

Słowa kluczowe

EN

cell line; protein level; polynucleotide phosphorylase; man; mitochondrion; treatment; interferon beta; melanoma

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2006
• Tom: 53
• Numer: 1
• Opis fizyczny: p.179-187,fig.,ref.

Twórcy

autor

Gewartowski K.

• Warsaw University, Warsaw, Poland

autor

Tomecki R.

autor

Muchowski L.

autor

Dmochowska A.

autor

Dzwonek A.

autor

Malecki M.

autor

Skurzak H.

autor

Ostrowski J.

autor

Stepien P.P.

Bibliografia

• Bedford FK, Julius D, Ingraham HA (1998) Neuronal expression of the 5HT3 serotonin receptor gene requires nuclear factor 1 complexes. J Neurosci 18: 6186–6194.
• Benoist C, O’Hare K, Breathnach R, Chambon P (1980) The ovalbumin gene-sequence of putative control regions. Nucleic Acids Res 8: 127–142.
• Borden EC, Hogan TF, Voelkel JG (1982) Comparative antiproliferative activity in vitro of natural interferons alpha and beta for diploid and transformed human cells. Cancer Res 42: 4948–4953.
• Chawla-Sarkar M, Leaman DW, Borden EC (2001) Preferential induction of apoptosis by interferon (IFN)-beta compared with IFN-alpha2: correlation with TRAIL/Apo2L induction in melanoma cell lines. Clin Cancer Res 6: 1821–1831.
• Cowell IG, Skinner A, Hurst HC (1992) Transcriptional repression by a novel member of the bZIP family of transcription factors. Mol Cell Biol 12: 3070–3077.
• Dorn A, Bollekens J, Staub A, Benoist C, Mathis D (1987) A multiplicity of CCAAT box-binding proteins. Cell 50: 863–872.
• Harada H, Fujita T, Miyamoto M, Kimura Y, Maruyama M, Furia A, Miyata T, Taniguchi T (1989) Structurally similar but functionally distinct factors, IRF-1 and IRF-2, bind to the same regulatory elements of IFN and IFN-inducible genes. Cell 58: 729–739.
• Horvath CM, Wen Z, Darnell JE Jr (1995) A STAT protein domain that determines DNA sequence recognition suggests a novel DNA-binding domain. Genes Dev 9: 984–994.
• Inagaki H, Matsushima Y, Ohshima M, Kitagawa Y (1997) Interferons suppress mitochondrial gene transcription by depleting mitochondrial transcription factor A (mt-TFA). J Interferon Cytokine Res 17: 263–269.
• Johns TG, Mackay IR, Callister KA, Hertzog PJ, Devenish RJ, Linnane AW (1992) Antiproliferative potencies of interferons on melanoma cell lines and xenografts: higher efficacy of interferon beta. J Natl Cancer Inst 84: 1185–1190.
• Kadonaga JT, Jones KA, Tjian R (1986) Promoter-specific activation of RNA polymerase II transcription by Sp1. Trends Biochem Sci 11: 20–23.
• Kortsaris A, Taylor-Papadimitriou J, Georgatsos JG (1976) Interferon inhibition of protein synthesis by isolated mitochondria. Biochem Biophys Res Commun 68: 1317–1322.
• Kulik J, Nowecki ZI, Rutkowski P, Ruka W, Rochowska M, Skurzak H, Siedlecki JA (2001) Detection of circulating melanoma cells in peripheral blood by a two-marker RT-PCR assay. Melanoma Res 11: 65–73.
• Le Roch KG, Johnson JR, Florens L, Zhou Y, Santrosyan A, Grainger M, Yan SF, Williamson KC, Holder AA, Carucci DJ, Yates JR 3rd, Winzeler EA (2004) Global analysis of transcript and protein levels across the Plasmodium falciparum life cycle. Genome Res 14: 2308–2318.
• Le Roy F, Bisbal C, Silhol M, Martinand C, Lebleu B, Salehzada T (2001) The 2-5A/RNase L/RNase L inhibitor (RLI) [correction of (RNI)] pathway regulates mitochondrial mRNAs stability in interferon alpha-treated H9 cells. J Biol Chem 276: 48473–48482.
• Leaman DW, Chawla-Sarkar M, Vyas K, Reheman M, Tamai K, Toji S, Borden EC (2002) Identification of X-linked inhibitor of apoptosis-associated factor-1 as an interferon-stimulated gene that augments TRAIL Apo2L-induced apoptosis. J Biol Chem 32: 28504–28511.
• Leszczyniecka M, Kang DC, Sarkar D, Su ZZ, Holmes M, Valerie K, Fisher PB (2002) Identification and cloning of human polynucleotide phosphorylase, hPNPase old-35, in the context of terminal differentiation and cellular senescence. Proc Natl Acad Sci USA 26: 16636–16641.
• Leszczyniecka M, Su ZZ, Kang DC, Sarkar D, Fisher PB (2003) Expression regulation and genomic organization of human polynucleotide phosphorylase, hPNPase(old-35), a type I interferon inducible early response gene. Gene 316: 143–156.
• Levy DE, Kessler DS, Pine R, Reich N, Darnell JE Jr (1988) Interferon-induced nuclear factors that bind a shared promoter element correlate with positive and negative transcriptional control. Genes Dev 2: 383–393.
• Matarrese P, Di Biase L, Santodonato L, Straface E, Mecchia M, Ascione B, Parmiani G, Belardelli F, Ferrantini M, Malorni W (2002) Type I interferon gene transfer sensitizes melanoma cells to apoptosis via a target activity on mitochondrial function. Am J Pathol 160: 1507–1520.
• Minczuk M, Lilpop J, Boros J, Stepien PP (2005) The 5’ region of the human hSUV3 gene encoding mitochondrial DNA and RNA helicase: promoter characterization and alternative pre-mRNA splicing. Biochim Biophys Acta 1729: 81–87.
• Nagaike T, Suzuki T, Katoh T, Ueda T (2005) Human mitochondrial mRNAs are stabilized with polyadenylation regulated by mitochondria-specific poly(A) polymerase and polynucleotide phosphorylase. J Biol Chem 20: 19721–19727.
• Paca-Uccaralertkun S, Zhao LJ, Adya N, Cross JV, Cullen BR, Boros IM, Giam CZ (1994) In vitro selection of DNA elements highly responsive to the human Tcell lymphotropic virus type I transcriptional activator, Tax. Mol Cell Biol 14: 456–462.
• Pearse RV 2nd, Collier LS, Scott MP, Tabin CJ (1999) Vertebrate homologs of Drosophila suppressor of fused interact with the gli family of transcriptional regulators. Dev Biol 212: 323–336.
• Piwowarski J, Grzechnik P, Dziembowski A, Dmochowska A, Minczuk M, Stepien PP (2003) Human polynucleotide phosphorylase, hPNPase, is localized in mitochondria. J Mol Biol 5: 853–857.
• Reece-Hoyes JS, Keenan ID, Pownall ME, Isaacs HV (2005) A consensus Oct1 binding site is required for the activity of the Xenopus Cdx4 promoter. Dev Biol 282: 509–523.
• Rooney RJ, Raychaudhuri P, Nevins JR (1990) E4F and ATF, two transcription factors that recognize the same site, can be distinguished both physically and functionally: a role for E4F in E1A trans activation. Mol Cell Biol 10: 5138–5149.
• Rosenblum MG, Yung WK, Kelleher PJ, Ruzicka F, Steck PA, Borden EC (1990) Growth inhibitory effects of interferon-beta but not interferon-alpha on human glioma cells: correlation of receptor binding, 2’,5’-oligoadenylate synthetase and protein kinase activity. J Interferon Res 10: 141–151.
• Sarkar D, Leszczyniecka M, Kang DC, Lebedeva IV, Valerie K, Dhar S, Pandita TK, Fisher PB (2003) Down-regulation of Myc as a potential target for growth arrest induced by human polynucleotide phosphorylase (hPNPaseold-35) in human melanoma cells. J Biol Chem 27: 24542–24551.
• Sarkar D, Lebedeva IV, Emdad L, Kang DC, Baldwin AS Jr, Fisher PB (2004) Human polynucleotide phosphorylase (hPNPaseold-35): a potential link between aging and inflammation. Cancer Res 20: 7473–7478.
• Scacheri PC, Rozenblatt-Rosen O, Caplen NJ, Wolfsberg TG, Umayam L, Lee JC, Hughes CM, Shanmugam KS, Bhattacharjee A, Meyerson M, Collins FS (2004) Short interfering RNAs can induce unexpected and divergent changes in the levels of untargeted proteins in mammalian cells. Proc Natl Acad Sci USA 101: 1892–1897.
• Schiller JH, Willson JK, Bittner G, Wolberg WH, Hawkins MJ, Borden EC (1986) Antiproliferative effects of interferons on human melanoma cells in the human tumor colony-forming assay. J Interferon Res 6: 615–265.
• Schrauwen P, Russell AP, Moonen-Kornips E, Boon N, Hesselink MK (2005) Effect of 2 weeks of endurance training on uncoupling protein 3 content in untrained human subjects. Acta Physiol Scand 183: 273–280.
• Shan B, Vazquez E, Lewis JA (1990) Interferon selectively inhibits the expression of mitochondrial genes: a novel pathway for interferon-mediated responses. EMBO J 9: 4307–4314.
• Soengas MS, Lowe SW (2003) Apoptosis and melanoma chemoresistance. Oncogene 22: 3138–3151.
• Tomecki R, Dmochowska A, Gewartowski K, Dziembowski A, Stepien PP (2004) Identification of a novel human nuclear-encoded mitochondrial poly(A) polymerase. Nucleic Acids Res 20: 6001–6014.
• Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3: research 00341–00411.
• Verrijzer CP, Alkema MJ, van Weperen WW, Van Leeuwen HC, Strating MJ, van der Vliet PC (1992) The DNA binding specificity of the bipartite POU domain and its subdomains. EMBO J 11: 4993–5003.
• Worm M, Schadendorf D, Czarnetzki BM (1993) Responsiveness to interferon treatment of human melanoma cells correlates to immunophenotype. Melanoma Res 3: 29–33.
• Wu L, Timmers C, Maiti B, Saavedra HI, Sang L, Chong GT (2001) The E2F1-3 transcription factors are essential for cellular proliferation. Nature 414: 457–462.
• Wysocki PJ, Karczewska A, Mackiewicz A (2002) Gene modified tumor vaccines in therapy of malignant melanoma. Otolaryngol Pol 56: 147–153.
• Yie J, Liang S, Merika M, Thanos D (1997) Intra- and intermolecular cooperative binding of high-mobility-group protein I(Y) to the β-interferon promoter. Mol Cell Biol 17: 3649–3662.
• Zhang L, Pagano JS (1997) IRF-7, a new interferon regulatory factor associated with Epstein-Barr virus latency. Mol Cell Biol 17: 5748–5757.
• Zhou S, Zawel L, Lengauer C, Kinzler KW, Vogelstein B (1998) Characterization of human FAST-1, a TGF beta and activin signal transducer. Mol Cell 2: 121–127.