PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2008 | 52 | 1 |

Tytuł artykułu

Antibiotic resistance patterns and resistance genes in enterococci isolated from sheep gastrointestinal tract in Slovakia

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Three hundred Enterococcus isolates from the rumen and faeces of three sheep were analysed for antibiotic resistance. Resistance to tetracycline, kanamycin, and streptomycin was predominant, followed by erythromycin resistance. About 7% of the isolates were resistant to ampicillin and vancomycin only. All tested isolates were susceptible to chloramphenicol. The presence of selected antibiotic resistance determinants was tested by PCR. No tested β-lactamase resistance determinant was detected in ampicillin resistant lactamase positive isolates. Both tet(M) and tet(L) tetracycline resistance determinants were detected. While tet(M) was predominant in both rumen and faecal isolates, clearly a higher frequency of tet(L) determinant was observed in rumen isolates. Among erythromycin resistant isolates, the erm(B) gene was detected only. From this data, it may be concluded that genotypically different enterococcal populations dwell in the upper and lower parts of sheep gastrointestinal tract.

Wydawca

-

Rocznik

Tom

52

Numer

1

Opis fizyczny

p.53-57,fig.,ref.

Twórcy

autor
  • Slovak Academy of Sciences, 040 01 Kosice, Slovak Republic
autor
autor

Bibliografia

  • 1. Bonafede M.E., Caria L.L., Ric L.B.: Enterococcal transposon Tn5384: evolution of a composite transposon through cointegration of enterococcal and staphylococcal plasmids. Antimicrob Agents Chemother 1997, 41, 1854- 1858.
  • 2. Chopra I., Roberts M.C.: Tetracycline antibiotics: mode of action, applications, molecular biology and epidemiology of bacterial resistance. Microbiol Mol Biol Rev 2001, 65, 232-260.
  • 3. Franz C.M.A.P., Muscholl-Silberhorn A.B., Yousif N.M.K., Vancanneyt M., Swings J., Holzapfel W.H.: Incidence of virulence factors and antibiotic resistance among enterococci isolated from food. App Environ Microbiol 2001, 67, 4385-1389.
  • 4. Jett B.D., Huycke M.M., Gilmore M.S.: Virulence of enterococci. Clin Microb Rev 1994, 7, 462-478.
  • 5. Jurkovic D., Krizkova L., Dusinsky R., Belicova A., Sojka M., Krajcovic J., Ebringer L.: Identification and characterization of enterococci from bryndza cheese. Lett Appl Microbiol 2006, 42, 553-559.
  • 6. Klare I., Konstabel C., Badstubner D., Werner G., Witte W.: Occurrence and spread of antibiotic resistances in Enterococcus faecium. Int J Food Microbiol 2003, 88, 269-290.
  • 7. Latini L., Ronchetti M.P., Merolla R., Guglielmi F., Bajaksouzian S., Villa M.P., Jacobs M.R., Ronchetti, R.: Prevalence of mef(E), erm and tet(M) genes in Streptococcus pneumoniae strains from central Italy. Antimicrob Agents Chemother 1999, 13, 29-33.
  • 8. Levinson E., Sudhakar M.: Increasing antimicrobial resistance: Therapeutic implications for enterococcal infections. Curr Infec Dis Rep 2000, 2, 417-423.
  • 9. Maniatis T., Fritsch E.F., Sambrook, J.: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1982, p. 445.
  • 10. McNamara E.B., King E.M., Smyth, E.G.: A survey of antimicrobial susceptibility of clinical isolates of Enterococcus spp. from Irish hospitals. J Antimicrob Chem 1995, 35, 185-189.
  • 11. Malik R., Ivan J., Javorsky P., Pristas, P.: Seasonal dynamics of antibiotic-resistant Enterobacteriaceae in the gastrointestinal tract of domestic sheep. Fol Microbiol 2005, 50, 349-352.
  • 12. Pospiech A., Neumann B.: A versatile quick prep of genomic DNA from Gram-positive bacteria. Trends Genet 1995, 11, 217-218.
  • 13. Portillo A., Ruiz-Larrea F., Zaragaza M., Alonso A., Martinez J.L., Torres C.: Macrolide resistance genes in Enterococcus spp. Antimicrob Agents Chemother 2000, 44, 967-971.
  • 14. Rice E.W., Messer J.W., Johnson C.H., Reasoner, D.J.: Occurrence of high-level aminoglycoside resistance in environmental isolates of enterococci. Appl Environ Microbiol 1995, 6, 374-376.
  • 15. Roberts M.C.: Epidemiology of tetracycline resistance determinants. Trends Microbiol 1994, 2, 353-357.
  • 16. Sidhu M.S., Heir E., Leegaard T., Wiger K., Hoick A.: Frequency of disinfectant resistance genes and genetic linkage with β-lactainasc transposon Tn552 among clinical staphylococci. Antimicrob Agents Chemother 2002, 46, 2797-2803.
  • 17. Sutcliffe G.: Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322. Proc Natl Acad Sci USA 1978, 75, 3737-3741.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-ad306b96-eb5a-476b-849f-8e1fb59df812
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.