Presence of potentially pathogenic Babesia sp. for human in Ixodes ricinus in Switzerland

• Autorzy: Casati S. , Sager H. , Gern L. , Piffaretti J.C.
• Języki publikacji: EN

Abstrakty

EN

We have designed and performed a new PCR method based on the 18S rRNA in order to individuate the presence and the identity of Babesia parasites. Out of 1159 Ixodes ricinus (Acari: Ixodidae) ticks collected in four areas of Switzerland, nine were found to contain Babesia DNA. Sequencing of the short amplicon obtained (411-452 bp) allowed the identification of three human pathogenic species: Babesia microti, B. divergens, for the first time in Switzerland, Babesia sp. EU1. We also report coinfections with B. sp. EU1-Borrelia burgdorferi sensu stricto and Babesia sp. EU1-B. afzelii.

Słowa kluczowe

EN

human disease; Babesia microti; pathogen; Switzerland; animal disease; Ixodes ricinus; Babesia divergens; tick

• Wydawca: -
• Czasopismo: Annals of Agricultural and Environmental Medicine
• Rocznik: 2006
• Tom: 13
• Numer: 1
• Opis fizyczny: p.65-70,fig.,ref.

Twórcy

autor

Casati S.

• Istituto Cantonale di Microbiologia, Via Mirasole 22A, 6500 Bellinzona, Switzerland

autor

Sager H.

autor

Gern L.

autor

Piffaretti J.C.

Bibliografia

• 1. Aeschlimann A, Brossard M, Quenet G: Contribution to the knowledge of Swiss piroplasmas. Acta Trop 1975, 32, 281-289.
• 2. Beati L, Keirans JE: Analysis of the systematic relationships among ticks of the genera Rhipicephalus and Boophilus (Acari: Ixodidae) based on mitochondrial 12S ribosomal DNA gene sequences and morphological characters. J Parasitol 2001, 87, 32-48.
• 3. Birkenheuer AJ, Levy MG, Breitschwerdt EB: Development and evaluation of a seminested PCR for detection and differentiation of Babesia gibsoni (Asian genotype) and B. canis DNA in canine blood samples. J Clin Microbiol 2003, 41, 4172-4177.
• 4. Casati S, Bernasconi MV, Gern L, Piffaretti JC: Diversity within Borrelia burgdorferi sensu lato genospecies in Switzerland by recA gene sequence. FEMS Microbiol Lett 2004, 238, 115-123.
• 5. Centeno-Lima S, do Rosario V, Parreira R, Maia AJ, Freudenthal AM, Nijhof AM, Jongejan F: A fatal case of human babesiosis in Portugal: molecular and phylogenetic analysis. Trop Med Int Health 2003, 8, 760-764.
• 6. Duh D, Petrovec M, Avsic-Zupanc T: Diversity of Babesia infecting European sheep ticks (Ixodes ricinus). J Clin Microbiol 2001, 39, 3395-3397.
• 7. Felsenstein J: Phylogenies from molecular sequences: inference and reliability. Annu Rev Genet 1988, 22, 521-565.
• 8. Foppa IM, Krause PJ, Spielman A, Goethert H, Gern L, Brand B, Telford SR, 3rd: Entomologie and serologic evidence of zoonotic transmission of Babesia microti, in eastern Switzerland. Emerg Infect Dis 2002, 8, 722-726.
• 9. Gern L, Aeschlimann A: Seroepidemiologic study of 2 foci of babesiosis of small mammals in Switzerland. Schweiz Arch Tierheilkd 1986,128, 587-600.
• 10. Gern L, Brossard M, Aeschlimann A, Broquet CA, Quenet G, Stucki JP, Ackermann J: Bovine piroplasmosis in the Clos-du-Doubs (Jura, Switzerland): preliminary observations. Schweiz Arch Tierheilkd 1982,124, 549-556.
• 11. Goethert HK, Telford SR, 3rd: What is Babesia microti? Parasitology 2003,127, 301-309.
• 12. Gorenflot A, Moubri K, Precigout E, Carey B, Schetters TP: Human babesiosis. Ann Trop Med Parasitol 1998, 92, 489-501.
• 13. Hartelt K, Oehme R, Frank H, Brockmann SO, Hassler D, Kimmig P: Pathogens and symbionts in ticks: prevalence of Anaplasma phagocytophilum (Ehrlichia sp.), Wolbachia sp., Rickettsia sp., and Babesia sp. in Southern Germany. Int J Med Microbiol 2004, 293 (Suppl 37), 86-92.
• 14. Herwaldt BL, Caccio S, Gherlinzoni F, Aspock H, Slemenda SB, Piccaluga P, Martinelli G, Edelhofer R, Hollenstein U, Poletti G, Pampiglione S, Loschenberger K, Tura S, Pieniazek NJ: Molecular characterization of a non-Babesia divergens organism causing zoonotic babesiosis in Europe. Emerg Infect Dis 2003, 9, 942-948.
• 15. Homer MJ, Aguilar-Delfin I, Telford SR, 3rd, Krause PJ, Persing DH: Babesiosis. Clin Microbiol Rev 2000, 13, 451-469.
• 16. Inokuma H, Yoshizaki Y, Shimada Y, Sakata Y, Okuda M, Onishi T: Epidemiological survey of Babesia species in Japan performed with specimens from ticks collected from dogs and detection of new Babesia DNA closely related to Babesia odocoilei and Babesia divergens DNA. J Clin Microbiol 2003, 41, 3494-3498.
• 17. Kjemtrup AM, Conrad PA: Human babesiosis: an emerging tick- borne disease. Int J Parasitol 2000, 30, 1323-1337.
• 18. Krause PJ, McKay K, Thompson CA, Sikand VK, Lentz R, Lepore T, Closter L, Christianson D, Telford SR, Persing D, Radolf JD, Spielman A: Disease-specific diagnosis of coinfecting tickborne zoonoses: babesiosis, human granulocytic ehrlichiosis, and Lyme disease. Clin Infect Dis 2002, 34, 1184-1191.
• 19. Kumur S, Tamura K, Jakobsen IB, Nei M: MEGA2: Molecular Evolutionary Genetics Analysis software. Arizona State University, Tempe, Arizona, USA, 2001
• 20. Meer-Scherrer L, Adelson M, Mordechai E, Lottaz B, Tilton R: Babesia microti infection in Europe. Curr Microbiol 2004, 48, 435-437.
• 21.Olmeda AS, Armstrong PM, Rosenthal BM, Valladares B, del Castillo A, de Armas F, Miguelez M, Gonzalez A, Rodriguez Rodriguez JA, Spielman A, Telford SR, 3rd: A subtropical case of human babesiosis. Acta Trop 1997, 67, 229-234.
• 22. Skotarczak B, Cichocka A: Isolation and amplification by polymerase chain reaction DNA of Babesia microti and Babesia divergens in ticks in Poland. Ann Agric Environ Med 2001, 8, 187-189.
• 23. Skotarczak B, Wodecka B, Cichocka A: Coexistence DNA of Borrelia burgdorferi sensu lato and Babesia microti in Ixodes ricinus ticks from north-western Poland. Ann Agric Environ Med 2002, 9, 25- 28.
• 24. Skrabalo Z, Deanovic Z: Piroplasmosis in man; report of a case. Doc Med Geogr Trop 1957, 9, 11-16.
• 25. Tsuji M, Wei Q, Zamoto A, Morita C, Arai S, Shiota T, Fujimagari M, Itagaki A, Fujita H, Ishihara C: Human babesiosis in Japan: epizootiologic survey of rodent reservoir and isolation of new type of Babesia microti-like parasite. J Clin Microbiol 2001, 39, 4316- 4322.
• 26. Zintl A, Mulcahy G, Skerrett HE, Taylor SM, Gray JS: Babesia divergens, a bovine blood parasite of veterinary and zoonotic importance. Clin Microbiol Rev 2003,16, 622-636.