Charakterystyka wybranych markerow patogennosci shigatoksycznych szczepow Escherichia coli wyosobnionych z surowej wolowiny przy uzyciu PCR

• Autorzy: Weiner M

Warianty tytułu

EN

Characterization of selected virulence markers of Shiga toxin-producing Escherichia coli originated from raw beef by the use of PCR

• Języki publikacji: PL

Abstrakty

EN

The aim of the study was the characterization of selected virulence markers of Shiga toxin-producing Escherichia coli originated from raw beef by the use of PCR. The identification of Stx variants (stx₂c, stx₂d, stx₂e and stx₂f) was done with the stx2 -positive E. coli strains, resulting in the detection of the stx2d gene in 13 STEC isolates out of which 3 strains also had the stx₂c gene. None of the isolates possessed the stx2f or stx2e genes. The intimin marker (γ variant) was observed in all E. coli O157:H7, whereas β variant in E. coli O26 isolates. The genotypic factors such as katP, toxB and efa1 were detected in all O157:H7 as well as in one O26 isolate. Fifteen STEC were iha-positive and five saa-positive. None of the saa-positive isolates belonged to the O157 or O26 groups. The simultaneous presence of the lpfAO₁₅₇/OI₋₁₄₁ and lpfAO₁₅₇/OI₋₁₅₄ genes was noted in 8 O157:H7 and in one O26 STEC. The lpfAO₁₅₇/OI₋₁₄₁ gene alone was present in 4 other STEC tested, including one E. coli O26. None of E. coli O157:H7 had the lpfAO113 marker that was observed in the all remaining STEC. In one isolate belonging to the E. coli O26 group, for the first time a simultaneous presence of the lpfAO₁₅₇/OI₋₁₄₁, lpfAO₁₅₇/OI₋₁₅₄ and lpfAO₁₁₃ was noted.

Słowa kluczowe

PL

zywnosc; mieso wolowe; zanieczyszczenia bakteryjne; Escherichia coli; szczepy shigatoksyczne; markery patogennosci; lancuchowa reakcja polimerazy

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2008
• Tom: 64
• Numer: 07
• Opis fizyczny: s.906-911,tab.,bibliogr.

Twórcy

autor

Weiner M

• Panstwowy Instytut Weterynaryjny - Panstwowy Instytut Badawczy, Al.Partyzantow 57, 24-100 Pulawy

Bibliografia

• 1.Brunder W., Schmidt H., Karch H.: KatP, a novel catalase-peroxidase encoded by the large plasmid of enterohaemorrhagic Escherichia coli O157:H7. Microbiology 1996, 142, 3305-3315.
• 2.Doughty S., Sloan J., Bennett-Wood V., Robertson M., Robins-Browne R. M., Hartland E. L.: Identification of a novel fimbrial gene cluster related to long polar fimbriae in locus of enterocyte effacement-negative strains of enterohemorrhagic Escherichia coli. Infect. Immun. 2002, 70, 6761-6769.
• 3.Eklund M., Leino K., Siitonen A.: Clinical Escherichia coli strains carrying stx genes: stx variants and stx-positive virulence profiles. J. Clin. Microbiol. 2002, 40, 4585-4593.
• 4.Imberechts H., de Greve H., Lintermans P.: The pathogenesis of edema disease in pigs. A review. Vet. Microbiol. 1992, 31, 221-233.
• 5.Janka A., Bielaszewska M., Dobrindt U., Karch H.: Identification and distribution of the enterohemorrhagic Escherichia coli factor for adherence (efa1) gene in sorbitol-fermenting Escherichia coli O157:H-. Int. J. Med. Microbiol. 2002, 292, 207-214.
• 6.Khan A., Yamasaki S., Sato T., Ramamurthy T., Pal A., Datta S., Chowdhury N. R., Sas S. C., Sikdar A., Tsukamoto T., Bhattacharya S. K., Takeda Y., Nair G. B.: Prevalence and genetic profiling of virulence determinants of non-O157 Shiga toxin producing Escherichia coli isolated from cattle, beef, and humans, Calcutta, India. Emerg. Infect. Dis. 2002, 8, 54-61.
• 7.Levine M. M.: Escherichia coli that cause diarrhea: enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent. J. Infect. Dis. 1987, 155, 377-389.
• 8.Lucchesi P. M. A., Kruger A., Parma A. E.: Distribution of saa variants in verocytotoxigenic Escherichia coli isolated from cattle and food. Res. Microbiol. 2006, 157, 263-266.
• 9.Morabito S., Tozzoli R., Oswald E., Caprioli A.: A mosaic pathogenicity island made up of the locus of enterocyte effacement and a pathogenicity island of Escherichia coli O157:H7 is frequently present in attaching and effacing E. coli. Infect. Immun. 2003, 71, 3343-3348.
• 10.Nawrotek P.: Wykorzystanie metody PCR-RFLP do wykrywania i różnicowania shigatoksycznych szczepów Escherichia coli na podstawie analizy genów slt. Medycyna Wet. 2003, 59, 35-39.
• 11.Nicholls L., Grant T. H., Robins-Browne R. M.: Identification of a novel genetic locus that is required for in vitro adhesion of a clinical isolate of enterohaemorrhagic Escherichia coli to epithelial cells. Mol. Microbiol. 2000, 35, 275-288.
• 12.Osek J.: Development of a multiplex PCR approach for the identification of Shiga toxin-producing Escherichia coli strains and their major virulence factor genes. J. Appl. Microbiol. 2003, 95, 1217-1225.
• 13.Osek J.: Genetic identification of virulence markers in Shiga toxin-producing Escherichia coli strains. Bull. Vet. Inst. Pulawy 2001, 45, 47-42.
• 14.Osek J., Weiner M.: Opracowanie testów multiplex PCR do identyfikacji odmian toksyny Shiga wytwarzanych przez Escherichia coli. Medycyna Wet. 2004, 60, 207-210.
• 15.Oswald E., Schmidt H., Morabito S., Karch H., Marches O., Caprioli A.: Typing of intimin genes in human and animal enterohemorrhagic and enteropathogenic Escherichia coli: characterization of a new intimin variant. Infect. Immun. 2000, 68, 64-71.
• 16.Paton A. W., Paton J. C.: Direct detection and characterization of Shiga toxigenic Escherichia coli by multiplex PCR for stx1, stx2, eae, ehxA, and saa. J. Clin. Microbiol. 2002, 40, 271-274.
• 17.Paton A. W., Srimanote P., Woodrow M. C., Paton J. C.: Characterization of Saa, a novel autoaglutinating adhesin produced by locus of enterocyte effacement-negative Shiga-toxigenic Escherichia coli strains that are virulent for humans. Infect. Immun. 2001, 69, 6999-7009.
• 18.Schmidt H., Zhang W. L., Hemmrich U., Jelacic S., Brunder W., Tarr P. I., Dobrindt U., Hacker J., Karch H.: Identification and characterization of a novel genomic island integrated at selC in locus of enterocyte effacement-negative, Shiga toxin-producing Escherichia coli. Infect. Immun. 2001, 69, 6863-6873.
• 19.Szalo I. M., Goffaux F., Pirson V., Pierard D., Ball H., Mainil J.: Presence in bovine enteropathogenic (EPEC) and enterohaemorrhagic (EHEC) Escherichia coli of genes encoding for putative adhesions of human EHEC strains. Res. Microbiol. 2002, 53, 653-658.
• 20.Tarr P. I., Bilge S. S., Vary J. C., Jelacic S., Habeeb R. L., Ward T. R., Baylor M. R., Besser T. E.: Iha: a novel Escherichia coli O157:H7 adherence-conferring molecule encoded on a recently acquired chromosomal island of conserved structure. Infect. Immun. 2000, 68, 1400-1407.
• 21.Tatarczak M., Wieczorek K., Posse B., Osek J.: Identification of putative adhesin genes in shigatoxigenic Escherichia coli isolated from different sources. Vet. Microbiol. 2005, 110, 77-85.
• 22.Toma C., Espinosa E. M., Song T., Miliwebsky E., Chinen I., Iyoda S., Iwanaga M., Rivas M.: Distribution of putative adhesions in different seropathotypes of Shiga toxin-producing Escherichia coli. J. Clin. Microbiol. 2004, 42, 4937-4946.
• 23.Torres A. G., Giron J. A., Perna N. T., Burland V., Blattner F. R., Avelino-Flores F., Kaper J. B.: Identification and characterization of lpfABCCDE, a fimbrial operon of enterohemorrhagic Escherichia coli O157:H7. Infect. Immun. 2002, 70, 5416-5427.
• 24.Wang G., Clark C., Rodgers G.: Detection in Escherichia coli of the genes encoding the major virulence factors, the genes defining the O157:H7 serotype, and components of the type 2 Shiga toxin family by multiplex PCR. J. Clin. Microbiol. 2002, 40, 3613-3619.
• 25.Weiner M.: Identyfikacja i charakterystyka shigatoksycznych szczepów Escherichia coli występujących w surowej wołowinie przy użyciu sondy molekularnej oraz multiplex PCR. Medycyna Wet. (wysłane do redakcji).
• 26.Zhang W. L., Kohler B., Oswald E., Beutin L., Karch H., Morabito S., Caprioli A., Suerbaum S., Schmidt H.: Genetic diversity of intimin genes of attaching and effacing Escherichia coli strains. J. Clin. Microbiol. 2002, 40, 4486-4492.
• 27.Zweifel C., Schumacher S., Blanco M., Blanco J. E., Tasara T., Blanco J., Stephan R.: Phenotypic and genotypic characteristics of non-O157 Shiga toxin-producing Escherichia coli (STEC) from Swiss cattle. Vet. Microbiol. 2005, 105, 37-45.