In vitro clonal propagation of Primula veris L. and preliminary phytochemical analysis

• Autorzy: Morozowska M , Wesolowska M.
• Języki publikacji: EN

Abstrakty

A method of in vitro clonal propagation of cowslip Primula veris L. (Primulaceae) is reported. This is a species of medicinal importance, protected by law in Poland. MS medium Murashige and Skoog (1962) with 6 benzyladenine BA (4.44 µM) and 2,4-dichlorophenoxyacetic acid 2,4-D (1.13 µM) was found to be optimal for in vitro cowslip propagation from shoot tips under the conditions of culture. Rooting was best in the presence of indole-3-butyric acid IBA (2.45 µM). In vitro seedlings were transferred to pots and then acclimated. After transplanting to a garden they showed further growth and development, including flowering and fruiting. Phytochemical analysis (2D-TLC) revealed that the flavonoid compounds in leafy shoots from in vitro culture were similar to those in leaves from field cultivation.

Słowa kluczowe

EN

in vitro; clonal propagation; Primula veris; phytochemical analysis; flavonoids; Primulaceae; medicinal plant

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2004
• Tom: 46
• Opis fizyczny: p.169-175,fig.,ref.

Twórcy

autor

Morozowska M

• August Cieszkowski Agricultural University, Wojska Polskiego 71C, 60-625 Poznan, Poland

autor

Wesolowska M.

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