Dolichyl sulphate and H-phosphonate: Enzymatic reactions with activated sugars

• Autorzy: Sizova O. V. , Maltsev S.D. , Shibaev V.N. , Jankowski W.J. , Chojnacki T.
• Języki publikacji: EN

Abstrakty

EN

Two phosphate-modified analogues of dolichyl phosphate were evaluated as substrates or inhibitors of the reactions catalyzed by mammalian microsomal enzymes. Dolichyl H-phosphonate could serve as an efficient acceptor for mannosyl and glucosyl transfer. The reaction products were chromatographically different from those formed from dolichyl phosphate. Lower activity of the H-phosphonate was observed for the reaction of N-acetylglucosaminyl phosphate transfer from UDP-GlcNAc. Dolichyl sulphate was shown not to serve as a substrate for the transfer of mannosyl (from GDP-Man), glucosyl (from UDP-Glc) or N-acetylglucosaminyl phosphate (from UDP-GlcNAc) residues in the presence of rat liver microsomes. Weak inhibitory properties of this analogue were demonstrated.

Słowa kluczowe

EN

enzymatic reaction; brain; glycosyltransferase; activated sugar; sugar; H-phosphonate; liver; dolichyl phosphate; dolichyl sulphate; microsome; rat

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1998
• Tom: 45
• Numer: 4
• Opis fizyczny: p.1021-1030,fig.

Twórcy

autor

Sizova O. V.

• Russial Academy of Sciences, Leninsky Prospect 47, Moscow V-334, Russia

autor

Maltsev S.D.

autor

Shibaev V.N.

autor

Jankowski W.J.

autor

Chojnacki T.

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