Characterization of the nitrate transporter in root plasma membranes of Cucumis sativus L.

• Autorzy: Klobus G , Jerzykiewicz J. , Buczek J.
• Języki publikacji: EN

Abstrakty

EN

Nitrate uptake in right-side out plasma membrane vesicles isolated from cucumber roots was characterized. Nitrate uptake into vesicles was driven by an artificially imposed pH gradient. The uptake was strongly inhibited by phenylglyoxal, an arginyl residue modificator. Only a slight repression of NO⁻₃ transport in vesicles was observed in the presence of NEM, a thiol group reagent. pCMBS, an other thiol reagent and DEPC, an effector of histidine residue, had no effect on the nitrate transport in plasma membranes. ATP-driven proton transport in vesicles was not significantly affected in the presence of both, phenylglyoxal and DEPC, whereas pCMBS and NEM abolished it almost completely. The possible role of the particular amino acids residues in the active nitrate transport is discussed. NO⁻₃ uptake into vesicles isolated from both, nitrate-induced and nitrate-depleted plant material was higher than that observed in the vesicles obtained from uninduced plants. Thus, isolated vesicles reflect the well-known in vivo response of intact plants on the exogenous nitrogen regime.

Słowa kluczowe

EN

plasma membrane; nitrate transport; cucumber; root; Cucumis sativus

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 1998
• Tom: 20
• Numer: 3
• Opis fizyczny: p.323-328

Twórcy

autor

Klobus G

• Wroclaw University, Wroclaw, Poland

autor

Jerzykiewicz J.

autor

Buczek J.

Bibliografia

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