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2007 | 56 | 4 |

Tytuł artykułu

RAPD techniques is a useful tool to distinguish Penicillium species

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Random amplified polymorphic DNA (RAPD) analysis was used to evaluate genetic diversity among 13 soil Pénicillium strains originating from widely dispersed areas. Twenty one of the 34 synthetic random primers were found to identify polymorphism in amplification products. The results show a high level of diversity of RAPD markers among the strains. All the strains could be identified by their characteristic amplification profile, using selected random primers. This suggests that RAPD analysis is a useful and reliable assay for characterizing the species of Pénicillium genus.

Wydawca

-

Rocznik

Tom

56

Numer

4

Opis fizyczny

p.273-276,fig.,ref.

Twórcy

autor
autor
autor

Bibliografia

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  • Jimenez M., R. Mateo, A. Querol, J. Mateo, and E. Hernandez. 1990. Differentiation of Penicillium griseofulvum Dierckx isolates by enzyme assays and by patulin and griseofulvin analysis. Appl. Environ. Microb. 56: 3718-3722.
  • Leach J., D.B. Finklstein and J.A. Ramosck. 1986. Rapid miniprep of DNA from filamentous fungi. Fungal Genetics Newsletter 33: 32-33.
  • Lee S.B. and J.W. Taylor.1990. Isolation of DNA from fungal mycelia and single spore. In: PCR Protocols. A. Guide to Methods and Applications. Eds., Innis, M.A., D.V. Gelfand, J.J. Sninsky and T.J. White. Academic Press Inc., New York, pp. 282-287.
  • Miller G.L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugars. Ann. Chem. 31: 426-428.
  • Nei M. and Li W.H. 1979. Mathematical model for studying genetic variation in terms of restricting endonucleases. Proc. Natl. Acad. Sci. USA 76: 5269-5273.
  • Oakey H.J., Gibson L.F. and A.M. George. 1999. DNA probes specific for Aeromonas hydrophila. J. Appl. Microb. 86: 187-193.
  • Pitt J.I. 1988. A Laboratory Guide to Common Penicillium Species. 2nd ed. CSIRO Division of Food Processing, North Ryde, New South Wales, Australia.
  • van de Zande L. and R. Bijlsma. 1995. Limitations of the RAPD technique in phylogeny reconstruction on Drosophila. J. Evol. Biol. 8: 645-656.

Typ dokumentu

Bibliografia

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bwmeta1.element.agro-article-a8288048-f7ba-4dd3-938f-f04248c72a6b
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