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2004 | 53 | 2 |

Tytuł artykułu

Detection of CMV infected cells by flow cytometry - evaluation of MAbs CCH2 and AAC10 directed against early and late CMV antigens

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
In this work evaluation of usefulness of monoclonal antibodies (MAbs) CCH2 and AAC10 directed against early - pUL44(DB52) and late - ppUL83(pp65) CMV antigens, utilized in Department of Virology, NIH for routine diagnosis of CMV infection by shell vial and pp65 antigenemia assay, for determination of CMV antigens by flow cytometry in human leucocytes, isolated, infected and cultivated in vitro was presented.

Wydawca

-

Rocznik

Tom

53

Numer

2

Opis fizyczny

p.127-129,fig.,ref.

Twórcy

autor
  • National Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland

Bibliografia

  • Boeckh M. and G. Boivin. 1998. Quantitation of cytomegalovirus: methodologic aspects and clinical applications. Clin. Microbiol. Rev. 11: 533-554.
  • Elmendorf S., J. McSharry, J. Laffin, D. Fogleman and J.M. Lehman. 2000. Detection of an early cytomegalovirus antigen with two-color quantitative flow cytometry. Cytometry 9: 254-260.
  • Essa S., A.S. Pacsa, R. Al-Attiyah, A. El-Shazly, R. Raghupathy and T. Said. 2000. The use of flow cytometry for the detection of CMV-specific antigen (pp65) in leucocytes of kidney recipients. Clin. Transplant. 14: 147-151.
  • Gerna G., M.G. Revello, E. Percivalle, M. Zavattoni, M. Parea and M. Battaglia. 1990. Quantification of human cytomegalovirus viremia by using monoclonal antibodies to different viral proteins. J. Clin. Microbiol. 28: 2681-2688.
  • Imbert-Marcille B.M., N. Robillard, A.S. Poirier, M. Coste-Burel, D. Cantarovich, N. Milpied and S. Billaudel. 1997. Development of a method for direct quantification of cytomegalovirus antigenemia by flow cytometry. J. Clin. Microbiol. 35: 2665-2669.
  • Poirier-Toulemonde A.S., N. Milpied, D. Cantarovich, J.F. Morcet, S. Billaudel and B.M. Imbert-Marcille. 2000. Clinical relevance of direct quantification of pp65 antigenemia using flow cytometry in solid organ and stem cell transplant recipients. J. Clin. Microbiol. 38: 3143-3149.
  • Schols D., R. Snoeck, J. Neyts and E. de Clercq. 1989. Detection of immediate early, early and late antigens of human cytomegalovirus by flow cytometry. J. Virol. Methods. 26: 247-254.
  • van der Bij W., R. Torensma, W.J. van Son, J. Anema, J. Schirm, A.M. Tegzess and T.H. The. 1988. Rapid immunodiagnosis of active cytomegalovirus infection by monoclonal antibody staining of blood leucocytes. J. Med. Virol. 25: 179-188.

Typ dokumentu

Bibliografia

Identyfikatory

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bwmeta1.element.agro-article-a6956f21-c0ae-4939-9781-50f9977b9813
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