Organophosphate insecticide chlorfenvinphos affects enzymatic and nonenzymatic antioxidants in erythrocytes and serum of rats

• Autorzy: Lukaszewicz-Hussain A , Moniuszko-Jakoniuk J.
• Języki publikacji: EN

Abstrakty

EN

Chlorfenvinphos, 2-chloro-1-(2,4-dichlorofenyl)vinyl diethyl phosphate, is an organophos-phate insecticide widely used in Poland singly or in mixture. The present study was undertaken to determine chlorfenvinphos-induced lipid peroxidation and to show whether acute intoxication with chlorfenvinphos alters the antioxidant system in the erythrocytes and serum. The study was conducted on male Wistar rats divided into two groups. The animals of control group were given olive oil intragastrically by a stomach tube, the animals of experimental group received oil solution of chlorfenvinphos (CVP) at a dose of 0.02, 0.1 or 0.5LD50. The blood was collected via heart puncture at the 1st, 24th and 48th hour after treatment. We determined the erythrocytes concentration of glutathione, the activities of glutathione peroxidase, glutathione reductase and glucose-6-phosphate dehydrogenase as well as the serum concentration of ascorbic acid, á-to-copherol and malondialdehyde. We observed the stimulation of enzymatic and nonenzymatic antioxi-dant system and lipid peroxidation in the erythrocytes and serum of chlorfenvinphos intoxicated rats.

Słowa kluczowe

EN

vitamin E; vitamin C; chlorfenvinphos; non-enzymatic antioxidant; serum; enzymatic antioxidant; erythrocyte; insecticide; organophosphate insecticide; lipid peroxidation; rat; mammalian cell

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2003
• Tom: 12
• Numer: 4
• Opis fizyczny: p.417-423

Twórcy

autor

Lukaszewicz-Hussain A

• Medical Academy, 2c Mickiewicza St., 15-222 Bialystok, Poland

autor

Moniuszko-Jakoniuk J.

Bibliografia

• 1. POPE C.N., Organophosphorus pesticides: do they all have the same mechanism of toxicity? J. Toxicol. Environm. Health 2, 161, 1999
• 2. HARRIS E.D., Regulation of antioxidant enzymes. FASEB J. 6, 2675, 1992
• 3. CHANCE B., SIES H., BOVERIS A., Hydroperoxide metabolism in mammalian organs. Physiol. Rev. 59, 527, 1979
• 4. RUTKOWSKI J., PLEWKA A., KAMIŃSKI M., Study on is chemic/reperfusion damage to the liver: hitherto existing successes and defeats. Acta Pol. Toxicol. 8, 1, 2000
• 5. ARAI M., IMAI H., KOUMURA T., YOSHIDA M., EMOTO K., UMEDA M., CHIBA N., NAKAGAWA Y., Mitochondrial phospholipid hydroperoxide glutathione per- oxidase plays a major role in preventing oxidative injury to cells. J. Biol. Chem. 274, 4924, 1999
• 6. ZHANG Z., APSE K., PANG J., STANTON Rt.C., High glucose inhibits glucose-6-phosphate dehydrogenase via cAMP in aortic endothelial cells. J. Biol. Chem. 275, 40042, 2000
• 7. ŁUKASZEWICZ-HUSSAIN A., MONIUSZKO-JAKO- NIUK J., Activity of lysosomal enzymes in acute intoxica­tion with organophosphorus insecticides. Pol. J. Environm. St. 6, 51, 1997
• 8. ŁUKASZEWICZ-HUSSAIN A., MONIUSZKO-JAKO- NIUK J., Glycolytical process in rat liver in chlorfenvinphos intoxication. Medycyna Pracy 5, 579, 1997
• 9. ŁUKASZEWICZ-HUSSAIN A., CHYCZEWSKI L., MONIUSZKO-JAKONIUK J., Concentration of lactate and glucose in blood serum and glycogen in liver in acute poisoning with chlorfenvinphos. Bromat. Chem. Toksykol. 31, 251, 1998
• 10. CHOW C.K., TAPPEL A.L., An enzymatic protective mechanism against lipid peroxidation damage to lung of ozone-exposed rats. Lipids 7, 518, 1972
• 11. ŁUKASZEWICZ-HUSSAIN A., Organophosphate insecti­cide chlorfenvinphos affects superoxide dismutase, catalase and malondialdehyde in rat liver. Pol. J. Environm. St. 10, 279, 2001
• 12. ROSS M.A., Determination of ascorbic acid and uric acid in plasma by high-performance liquid chromatography. J. Chromathog. B. 657, 197, 1994
• 13. KONNEH M.K., RUTHERFORD C., LI S., ANGGARD E.E., FERNS G.A.A., Vitamin E inhibits the intimal re­sponse to ballon catheter injury in the carotid artery of the cholesterol-fed rat. Atherosclerosis 113, 29, 1995
• 14. BUEGE J.A., AUST S., Microsomal lipid peroxidation. Methods Enzymol. 51, 302, 1978
• 15 TAVAZZI B., DI PIERRO D., AMORINI A.M., FAZZINA G., TUTTOBENE M., GIARDINA B., LAZZARINO G., Energy metabolism and lipid peroxidation of human erythrocytes as a function of increased oxidative stress. Eur. J. Biochem. 267, 684, 2000
• 16. MALMEZAT T., BREUILLE D., CAPITAN P., MIRAND P.P., OBLED Ch., Glutathione turnover is increased during the acute phase of sepsis in rats. J. Nutr. 130, 1239, 2000
• 17. PARKE D.V., PIOTROWSKI J.K., Glutathione: its role in detoxication of reactive oxygen and environmental chemi­cals. Acta Pol. Toxicol. 4, 1, 1996
• 18. PARKE D.V., Nutritional requirements for detoxication of environmental chemicals. Food Add. Contam. 8, 381, 1991
• 19. BEUTLER E., Nutritional and metabolic aspects of glutathi­one. Ann. Rev. Nutr. 9, 287, 1989
• 20. SOPLARICS Z., WU J-X., Role of glutathione and catalase in H2O2 detoxification in LPS-activated hepatic endothelial and Kupffer cells. Am. J. Physiol. 273, G1304, 1997
• 21. GAETANI G.F., GALIANO S., CANEPA S., FERRARIS A.M., KIRKMAN H.N., Catalase and glutathione peroxidase are equally active in detoxification of hydrogen perox­ide in human erythrocytes. Blood 73, 334, 1994
• 22. KAPLOVITZ N., TSUKAMOTO H., Oxidative stress and liver desease. Prog. Liver Dis. 14, 131, 1996
• 23. BABIOR B.M., Superoxide: a two-edged sword. Braz. J. Med. Biol. Res. 30, 141, 1997
• 24. COHEN H.J., AVISSAR N., Molecular and biochemical aspects of selenium metabolism and deficiency. Progr. Biol. Research 380, 192, 1993
• 25. FLOHE L., Glutathione peroxidase. Basic Life Sc. 49, 663, 1988
• 26. SALVEMINI F., FRANZE A., IERVOLINO A., FILOSA S., SALZANO S., URSINI M.V, Enhanced glutathione levels and oxidoresistance mediated by increased glucose-6-phosphate dehydrogenase expression J. Biol. Chem. 274, 2750, 1999
• 27. TIAN W.N., BRAUSTEIN L.D., APSE K., PANG J., ROSE M., Tian X., Stanton R.C., Importance of glucoses-phos­phate dehydrogenase activity in cell death. Am. J. Physiol. 276, C1121, 1999
• 28. BUS J.S., VINEGAR A., BROOKS S.M., Biochemical and physiological changes in lungs of rats exposed to a cadmium chloride aerosol. Am Rev. Respir. Desease 118, 573, 1978
• 29. FREI B., ENGLAND L., AMES B.N., Ascorbate is an out­standing antioxidant in human blood plasma. Proc. Nat. Ac. Sc. USA 86, 6337, 1989
• 30. MEISTER A., Glutathione-ascorbic acid antioxidant system in animals. J. Biol. Chem. 269, 9397, 1994
• 31. SIES H., MURPHY M.E., Role of tocopherols in the pro­tection of biological system against oxidative damage. J. Photochem. Photobiol. B, Biology 8, 211, 1991
• 32. VANDUIJN M.M., TIJSSEN K., VANSTEVENINCK J., VAN DEN BROEK P.J.A., VAN DER ZEE J., Erythrocytes reduce extracellular ascorbate free radicals using extracel­lular ascorbate as an electron donor. J. Biol. Chem. 275, 27720, 2000
• 33. CATHCART R.F.: A unique function for ascorbate. Med. Hypotheses 35, 32, 1991