Effect of electromagnetic waves on susceptibility of fungi of the genus Candida to miconazole

• Autorzy: Kurnatowski P , Klimiuk C. , Glowacka A.
• Języki publikacji: EN

Abstrakty

EN

Increasing use of elctromagnetic fields (EF) in the treatment of various diseases may have potential impacts on fungi - possible aetiological factors of concomitant mycoses. The aim of this study was to investigate the effects of EF on miconazole susceptibility among fungi of the genus Candida showing confirmed pathogenicity in humans. Fifteen Candida strains obtained from patients were used and their susceptibility to miconazole was determined by diffusion in agar gel. Antifungal activity of miconazole was assessed as minimal inhibitory concentration (MIC) which was calculated for Candida strains not exposed to EF (control) and EF-exposed (3 experiments). In the majority of cases the susceptibility of Candida species to miconazole decreased (higher MICs) after the first week of EF exposure, regardless of the EF's parameters. This was followed by an increase in susceptibility (lower MICs) after the second week of exposure to EF of 2 mT intensity and frequency 3 Hz (experiment I) and an EF of 9 mT intensity and 12.5 Hz frequency (experiment II) relative to control. An increase in susceptibility (lower MICs) was observed in the second week of exposure, whatever the parameters of the EF. The application of low intensity, low frequency EF for a period of at least two weeks may be beneficial in the treatment of mycoses caused by pathogenic fungi of Candida genus.

Słowa kluczowe

EN

miconazole; electromagnetic wave; susceptibility; pathogenic fungi; Candida; fungi

• Wydawca: -
• Czasopismo: Wiadomości Parazytologiczne
• Rocznik: 2003
• Tom: 49
• Numer: 1
• Opis fizyczny: p.61-71,fig.,ref.

Twórcy

autor

Kurnatowski P

• Medical University, Pl.Hallera 1, 90-647 Lodz, Poland

autor

Klimiuk C.

autor

Glowacka A.

Bibliografia

• Adey W.R. 1981. Tissue interaction with nonionizing electromagnetic fields. Physiology Review 61: 435-514.
• Adey W.R. 1993. Biological effects of electromagnetic field. Journal of Cellular Biochemistry 51: 410-416.
• Antonow O.E., Kozyreva E.V., Svishcheva T.I., Goncharova N.V. 1997. The destruction of microscopic organisms by their irradiation with a special form of UHF electromagnetic signals. Izvistija Akademii Nauk Serija Biologiceskaja 11-12: 728-734.
• Budak A., Zyss T., Bogusz B., Głowacka A. 1998. Wpływ zmiennych pól magnetycznych na wzrost niektórych grzybów patogennych. Alpe Adria Microbiology Journal 7: 191-196.
• Budak A., Zyss T., Bogusz B., Ratka P., Odziewa-Krzyszakowska D. 1997. Wpływ zmiennych pó1 magnetycznych o indukcji 0.01 i 0.1 T na wzrost niektórych grzybów patogennych skóry: badania in vitro. Przegląd Dermatologiczny 84: 317-323.
• Budak A., Zyss T., Bogusz B. 1996. Bezpieczenstwo magnetoterapii w zakazeniach grzybami patogennymi skóry: badania in vitro - wyniki wstępne. Mikologia lekarska 3: 245-248.
• Howlett J.A., Sguier C.A. 1980. Candida albicans ultrastructure: colonization and invasion of oral epithelium. Infection and Immununity 29: 252-260.
• Kadłubowski R. 1971. Approximate methods in analysis of dose/response curve. Abstracts of VI International Congres of Chemioteraphy, Praha, A-12/28.
• Kirschvink J., Kobayashi 1992. Magnetite in human tissues: a mechanism for the biological effects of weak ELF magnetic fields. Bioelectromagnetics 1: 101-113.
• Kozakov V., Krasilnikov A.P. 1986. Influence of combined use of laser irradiation and variable magnetic field on multiplication of Mycobacterium tuberculosis. Problemy Tuberkuleza 10: 62-64.
• Kurnatowska A.1995. Wybrane zagadnienia mikologii medycznej. Promedi, Łódź.
• Mamos K. 1999. Wpływ rezonansowego pola magnetycznego (magnetostymulacji) na właściwości biologiczne grzybów z rodzaju Candida zasiedlających błony śluzowe i skórę. WAM, Łódź.
• Moore R.L. 1979. Biological effects of magnetic fields: studies with microorganism. Canadian Jurnal of Microbiology 10: 1145-1151.
• Pawlik B. 1980. Oznaczanie wrażliwości grzybów drożdżopodobnych na chemioterapeutyki książkową metodą dyfuzyjną. Diagnostyka Laboratoryjna 16: 273-278.
• Ramon C., Martin J.T., Powell M.R. 1987. Low-level magnetic-field - induced growth modification of Bacillus subtilis. Bioelectromagnetics 8: 275-282.
• Riabtseva Y.L., Kuzminski B.N. 1981. Effect of a pulsative field on Shigella flexneri. Zhurnal Mikrobiologii, Epidemiologii i Immunobiologii 4: 106-109.
• Sieron A., Cieślar G., Adamek M. 1994. Magnetoterapia i laseroterapia. Wydawnictwo ŚIAM, Katowice.
• Schwartz E., Svejnochova M., Siposowa E. 1989. Different effects of a weak static and a weak rotating magnetic field on the growth of mycobacteria. Bratislavske Lekarske Listy 11: 787-792.
• Valberg P., Kavet R., Rafferty C. 1997. Can low level 50/60 Hz electric and magnetic fields cause biological effects? Radiation Research 6: 2-21.
• White P.J., Barker A.T., Foulds J.S. 1982. Sensitivity of several bacteria and fungi to the magnetic field generated by a bone stimulator. European Journal of Clinical Microbiology 4: 225-256.
• Zaremba M.L., Borowski J. 1994. Podstawy mikrobiologii lekarskiej. PZWL, Warszawa.